Detection of P-glycoprotein in the Golgi apparatus of drug-untreated human melanoma cells

International Journal of Cancer

Volumn 75, Issue 6, 1998, Pages 885-893

  Molinari, Agnese ^a   Calcabrini, Annarica ^a   Meschini, Stefania ^a   Stringaro, Annarita ^a   Del Bufalo, Donatella ^b   Cianfriglia, Maurizio ^a   Arancia, Giuseppe ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^b REGINA ELENA NATIONAL CANCER INSTITUTE   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ANTHRACYCLINE ANTIBIOTIC AGENT; CYCLOSPORIN A; CYTOTOXIC AGENT; DOXORUBICIN; GENE PRODUCT; GENISTEIN; GLYCOPROTEIN P; MESSENGER RNA; PROBENECID; VERAPAMIL;

ARTICLE; CELLULAR DISTRIBUTION; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; CYTOPLASM; DRUG ACCUMULATION; DRUG DISTRIBUTION; DRUG SENSITIVITY; DRUG TRANSPORT; FLOW CYTOMETRY; GOLGI COMPLEX; HUMAN; HUMAN CELL; IMMUNOELECTRON MICROSCOPY; IMMUNOFLUORESCENCE MICROSCOPY; INTRACELLULAR TRANSPORT; MELANOMA CELL; MULTIDRUG RESISTANCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

BIOLOGICAL TRANSPORT; CELL COMPARTMENTATION; CELLS, CULTURED; CYCLOSPORINE; DNA-BINDING PROTEINS; DOXORUBICIN; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GENE EXPRESSION; GOLGI APPARATUS; HUMANS; MELANOMA; MICROSCOPY, CONFOCAL; MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS; P-GLYCOPROTEIN; PROBENECID; RNA, MESSENGER; RNA, NEOPLASM; TUMOR CELLS, CULTURED;

EID: 0031941406     PISSN: 00207136     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0215(19980316)75:6<885::AID-IJC11>3.0.CO;2-2     Document Type: Article

References (25)

1. BALDINI, N., SCOTLANDI, K., SERRA, M., SHIKITA, T., ZINI, N., OGNIBENE, A., SANTI, S., FERRACINI, R. and MARALDI, N.M., Nuclear immunolocalization of P-glycoprotein in multidrug-resistant cell lines showing similar mechanisms of doxorubicin distribution. Europ. J. Cell Biol., 68, 226-239 (1995).
2. BERGER, W., ELBLING, L., MINAI-POUR, M., VETTERLEIN, M., PIRKER, R., KOKOSCHKA, E.-M. and MICKSCHE, M., Intrinsic MDR1-gene and P-glycoprotein expression in human melanoma cell lines. Int. J. Cancer, 59, 717-723 (1994).
3. BERGER, W., HAUPTMANN, E., ELBLING, L., VETTERLEIN, M., KOKOSCHKA, E.M. and MICKSCHE, M., Possible role of the multidrug-resistance-associated protein (MRP) in chemoresistance of human melanoma cells. Int. J. Cancer, 71, 108-115 (1997).
4. CALCABRINI, A., VILLA, A.M., MOLINARI, A., DOGLIA, S.M. and ARANCIA, G., Influence of N-methylformamide on the intracellular transport of doxorubicin. Europ. J. Cell Biol., 72, 61-69 (1997).
5. CHOMCZYNSKI, P. and SACCHI, N., Single-step method of RNA isolation by acid-guanidium-thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162, 156-159 (1987).
6. CIANFRIGLIA, M., WILLINGHAM, M.C., TOMBESI, M., SCAGLIOTTI, V., FRASCA, G. and CHERSI, A., P-glycoprotein mapping. I. Identification of a linear human-specific epitope in the fourth loop of the P-glycoprotein extracellular domain by MM4.17 murine monoclonal antibody to human multi-drug-resistant cells. Int. J. Cancer, 56, 153-160 (1994).
7. COAN, D.E., WECHEZACK, A.R., VIGGERS, R.F. and SAUVAGE, L.R., Effect of shear stress upon localization of the Golgi apparatus and microtubule organizing center in isolated cultured endothelial cells. J. Cell Sci., 104, 1145-1153 (1993).
8. COLE, S.P.C., BHARDWAJ, G., GERLACH, J.H., MACKIE, J.E., GRANT, C.E., ALMQUIST, K.C., STEWART, A.J., KURZ, E.U., DUNCAN, A.M.V. and DEELEY, R.G., Over-expression of a transporter gene in a multidrug-resistant human lung-cancer cell line. Science, 258, 1650-1654 (1992).
9. FELLER, N., BROXTERMAN, H.J., WAHRER, D.C.R. and PINEDO, H.M., ATP-dependent efflux of calcein by the multidrug-resistance protein (MRP): no inhibition by intracellular glutathione depletion. FEBS Lett., 368, 385-388 (1995).
10. FLENS, M.J., IZQUIERDO, M.A., SCHEFFER, G.L., FRITZ, J.M., MEIJER, C.J.L.M., SCHEPER, R.J. and ZAMAN, G.J.R., Immunochemical detection of the multidrug-resistance-associated protein MRP in human multidrug-resistant tumor cells by monoclonal antibodies. Cancer Res., 54, 4557-4563 (1994).
11. GARBE, C., Chemotherapy and chemo-immunotherapy in disseminated malignant melanoma. Melanoma Res., 3, 291-299 (1993).
12. GOTTESMAN, M.M. and PASTAN, I., Biochemistry of multidrug resistance mediated by the multidrug transporter. Ann. Rev. Biochem., 62, 385-427 (1993).
13. HAMADA, H. and TSURUO, T., Functional role of the 170-180 kD glycoprotein specific to drug-resistant tumor cells as revealed by monoclonal antibodies. Proc. nat. Acad. Sci. (Wash.), 83, 7785-7789 (1986).
14. HINDENBURG, A.A., GERVASONI, J.E. JR., KRISHNA, S., STEWART, V.J., ROSADO, M., LUTZKY, J., BHALA, K., BAKER, M.A. and TAUB, R.N., Intracellular distribution and pharmacokinetics of daunorubicin in anthracycline-sensitive and -resistant HL60 cells. Cancer Res., 49, 4607-4614 (1989).
15. MESCHINI, S., MOLINARI, A., CALCABRINI, A., CITRO, G. and ARANCIA, G., Intracellular localization of the amitumor drug adriamycin in living cultured cells: a confocal microscopy study. J. Microsc., 176, 204-210 (1994).
16. MOLINARI, A., CIANFRIGLIA, M., MESCHINI, S., CALCABRINI, A. and ARANCIA, G., P-glycoprotein expression in the Golgi apparatus of multidrug-resistant cells. Int. J. Cancer, 59, 789-795 (1994).
17. NOONAN, K.E., BECK, C., HOLZMAYER, T.A., CHIN, J.E., WUNDER, J.S., ANDRULIS, I.L., GADZAR, A.F., WILLMAN, C.L., GRIFFITH, B., VON HOFF, D.D. and RONINSON, I.B., Quantitative analysis of MDR1 (multidrug-resistance)-gene expression in human tumors by polymerase chain reaction. Proc. nat. Acad. Sci. (Wash.), 87, 7160-7164 (1990).
18. RUTHERFORD, A.V. and WILLINGHAM, M.C., Ultrastructural localization of daunomycin in multidrug-resistant cells with modulation of the multidrug transporter. J. Histochem. Cytochem., 41, 1573-1577 (1993).
19. SAEKI, T., UEDA, K., TANIGAWARA, Y., HORI, R. and KOMANO, T., Human P-glycoprotein transport cyclosporin A and FK506. J. biol. Chem., 268, 6077-6080 (1993).
20. SAFA, A.R., GLOVER, C.J., SEWELL, J.L., MEYERS, M.B., BIEDLER, J.L. and FELSTED, R.L., Identification of the multidrug-resistance-related membrane glycoprotein as an acceptor for calcium blocker. J. biol. Chem., 262, 7884-7888 (1987).
21. SCHADENDORF, D., MAKKI, A., STAHR, C., VAN DICK, A., WANNER, R., SCHEFFER, G.L., FLENS, M.J., SCHEPER, R. and HENZ, B.M., Membrane transport proteins associated with drug resistance expressed in human melanoma. Amer. J. Pathol., 147, 1545-1552 (1995).
22. SCHADENDORF, D., WORM, M., ALGERMISSEN, B., KOHLMUS, C.M. and CZARNETZKI, B.M., Chemosensitivity testing of human melanoma cells: retrospective analysis of clinical response and in vitro drug sensitivity. Cancer, 73, 103-108 (1994).
23. SCHEPER, R.J., BROXTERMAN, H.J., SCHEFFER, J.L., KAAIJK, P., DALTON, W.S., VAN HEININGEN, T.H.M., VAN KALKEN, C.K., SLOVACK, M.L., DE VRIES, E.G.E., VAN DE VALK, P., MEIJER, C.J.L.M. and PINEDO, H.M., Over-expression of a 110-kD vesicular protein in non-P-glycoprotein-mediated multidrug resistance. Cancer Res., 53, 1475-1479 (1993).
24. VERSANTVOORT, C.H.M., SCHUURHUIS, G.J., PINEDO, H.M., EEKMAN, C.A., KUIPER, C.M., LANKELMA, J. and BROXTERMAN, H.J., Genistein modulates the decreased drug accumulation in non-P-glycoprotein-mediated multidrug-resistant tumour cells. Brit. J. Cancer, 68, 939-946 (1993).
25. WILLINGHAM, M.C., RICHERT, N.D., CORNWELL, M.M., TSURUO, T., HAMADA, H., GOTTESMAN, M.M. and PASTAN, I.H., Immunocytochemical localization of P170 at the plasma membrane of multidrug-resistam human cells. J. Histochem. Cytochem., 35, 1451-1456 (1987).