Ultrasensitive in vivo bioassay detects bioactive human growth hormone in transduced primary human keratinocytes

Journal of Endocrinological Investigation

Volumn 21, Issue 1, 1998, Pages 1-6

  Bellini, M H ^a   Mathor, M B ^b   De Luca, M ^c   Cancedda, R ^b   Bartolini, P ^a  

^a INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES   (Brazil)

^b CENTRO DI BIOTECNOLOGIE AVANZATE   (Italy)

^c LABORATORIO DI PATOLOGIA VASCOLARE   (Italy)

Author keywords

'in vivo' bioassay; 'little' mice; Recombinant growth hormone; Transduced keratinocytes

Indexed keywords

HUMAN GROWTH HORMONE;

ACCURACY; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIUM; BIOASSAY; CONTROLLED STUDY; CULTURE MEDIUM; DRUG ACTIVITY; EPIDERMIS CELL; FEMALE; GENE THERAPY; GENETIC TRANSDUCTION; GROWTH CURVE; HUMAN; HUMAN CELL; HYPOPHYSECTOMY; KERATINOCYTE; MOUSE; NONHUMAN; RAT; TIBIA; WEIGHT GAIN;

EID: 0031939238     PISSN: 03914097     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF03347278     Document Type: Article

References (24)

1. Bellini M.H., Bartolini P. In vivo bioassay for the potency determination of human growth hormone in dwarf "little" mice. Endocrinology 132: 2051, 1993.
2. Bristow A.F., Gaines-Das R., Jeffcoate S.L., Schulster D. The first international standard for somatotropin: report of an international collaborative study. Growth Reg. 5: 133, 1995.
3. Greenspan F.S., Li C.H., Simpson M.E., Evans H.M. Bioassay of hypophyseal growth hormone: the tibia test. Endocrinology 45: 455, 1949.
4. Papkoff H., Li C.H. Hypophyseal growth hormone. In: Dorfman R.I. (Ed.), Methods in Hormone Research. Academic Press, New York, 1962, vol. 2, p. 671.
5. Morgan J.R., Barrandon Y., Green H., Mulligan R.C. Expression of an exogenous growth hormone gene by transplantable human epidermal cells. Science 237: 1476, 1987.
6. Teumer J., Lindahl A., Green H. Human growth hormone in the blood of athymic mice grafted with cultures of hormone-secreting human keratinocytes. FASEB J. 4: 3245, 1990.
7. Vogt P.M., Thomson S., Andree C., Liu P., Breuing K., Hatzis D., Brown H., Mulligan R.C., Eriksson E. Genetically modified keratinocytes transplanted to wounds reconstitute the epidermis. Proc. Natl. Acad. Sci. USA. 91: 9307, 1994.
8. Jensen V.B., Jensen T.G., Jensen P.K.A., Rygaard J., Hansen B.S., Fogh J., Kolvraa S., Bolund L. Gene transfer into cultured human epidermis and its transplantation onto immunodeficient mice: an experimental model for somatic gene therapy. J. Invest. Dermatol. 103: 391, 1994.
9. 125I-human growth hormone to specific receptors in human cultured lymphocytes. J. Biol. Chem. 249: 1661, 1974.
10. Chen B.F., Chang W.C., Chen ST., Chen D.S., Hwang L.H. Long-term expression of the biologically active growth hormone in genetically modified fibroblasts after implantation into a hypophysectomized rat. Hum. Gene Ther. 6: 917, 1995.
11. Mathor M.B. Gene expression studies with human growth hormone gene transfected cells for a possible utilization in gene therapy. Ph D thesis work, IPEN-CNEN/ University of São Paulo-Brazil, 1994.
12. Eicher E.M., Beamer W.G. Inherited ateliotic dwarfism in mice. J. Hered. 67: 87, 1976.
13. Jansson J.O., Downs T.R., Beamer W.G., Frohman L.A. Receptor associated resistance to growth hormone-releasing factor in dwarf "little" mice. Science 232: 511, 1986.
14. Wilson D.B., Wyatt D.P., Gadler R.M., Baker C.A. Quantitative aspects of growth hormone cell maturation in the normal and little mutant mouse. Acta. Anat. 131: 150, 1988.
15. Zarrow M.X., Yochim J.M., Mc Carthy J.L. Experimental Endocrinology. A sourcebook of Basic Techniques. Academic Press, New York, 1964, p. 308.
16. Bartolini P., Marques de Assis L., Schwarz I., Pieroni R.R. An accurate determination of human growth hormone content in different pituitary extracts, using a radioimmunoassay with polyacrylamide gel electrophoresis as a bound-free separation system. Clin. Chim. Acta 79: 223, 1977.
17. Lowry D.B., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265, 1951.
18. Ribela M.T.C.P., Bartolini P. The use of recombinant human growth hormone for radioiodination and standard preparation in radioimmunoassay. J. Immunol. Methods 159: 269, 1993.
19. Zambruno G., Marchisio P.C., Marconi A., Vaschieri C., Melchiori A., Giannetti A., De Luca M. Transforming growth factor-β1 modulates β1 and β5 integrin receptors and induces the de novo expression of the α v β heterodimer in normal human keratinocytes: implications for wound healing. J. Cell Biol. 129: 853, 1995.
20. Bartolini P., Muramoto E. Bioassay of hGH: standardization of more economical assay designs. Horm. Metab. Res. 16: 614, 1984.
21. Rodbard D. Statistical estimation of the minimal detectable concentration (sensitivity) for radioligand assays. Anal. Biochem. 90: 1, 1978.
22. Wilhelmi A.E. Growth hormone bioassay. In: Berson S.A., Yalow R.S. (Eds.), Methods in Investigative and Diagnostic Endocrinology. North Holland, Amsterdam, 1973, vol. 2A, p. 296.
23. Groesback M.D., Parlow A.F. Highly improved precision of the hypophysectomized female rat body weight gain bioassay for growth hormone by increased frequency of injections, avoidance of antibody formation, and other simple modifications. Endocrinology 120: 2582, 1987.
24. European Pharmacopoeia Convention. Human growth hormone for injection. In: Maisonneuve S.A. (Ed.), European Pharmacopoeia, ed 2, part II, 11th fascicule. Sainte Ruffine, France, 1987, p. 556.1.