Supernatant cytotoxicity and proteolytic activity of selected oral bacteria against human gingival fibroblasts in vitro

Archives of Oral Biology

Volumn 43, Issue 1, 1998, Pages 15-23

  Letzelter, Corinne ^a   Croute, Françoise ^a   Pianezzi, Bernadette ^a   Roques, Christine ^a   Soleilhavoup, Jean Pierre ^a  

^a UNIVERSITÉ DE TOULOUSE   (France)

Author keywords

Collagen; Endodontic bacteria; Exotoxins; Fibronectin; Human gingival fibroblasts; Protease release

Indexed keywords

BACTERIAL TOXIN; COLLAGEN; CYTOTOXIN; FIBRONECTIN; PEPTIDE HYDROLASE;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; ARTICLE; BACTERIAL PHENOMENA AND FUNCTIONS; BACTERIUM; BACTERIUM CULTURE; CAPNOCYTOPHAGA; CELL DIVISION; CHEMISTRY; CONTROLLED STUDY; CULTURE MEDIUM; CYTOTOXICITY; ENZYMOLOGY; EXTRACELLULAR MATRIX; FIBROBLAST; FLUORESCENT ANTIBODY TECHNIQUE; GINGIVA; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; GROWTH INHIBITION; HUMAN; HUMAN CELL; IMMUNOELECTROPHORESIS; IMMUNOFLUORESCENCE; IN VITRO STUDY; METABOLISM; MICROBIOLOGY; MOUTH FLORA; PATHOLOGY; PEPTOSTREPTOCOCCUS; PREVOTELLA; PROTEIN DEGRADATION; SUPERNATANT; TOOTH PULP;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; BACTERIA; BACTERIAL PHYSIOLOGY; BACTERIAL TOXINS; CAPNOCYTOPHAGA; CELL DIVISION; COLLAGEN; CULTURE MEDIA, CONDITIONED; CYTOTOXINS; DENTAL PULP; EXTRACELLULAR MATRIX; FIBROBLASTS; FIBRONECTINS; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GINGIVA; HUMANS; IMMUNOELECTROPHORESIS; PEPTIDE HYDROLASES; PEPTOSTREPTOCOCCUS; PREVOTELLA;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; BACTERIA (MICROORGANISMS); CAPNOCYTOPHAGA; CAPNOCYTOPHAGA OCHRACEA; NEGIBACTERIA; PEPTOSTREPTOCOCCUS; POSIBACTERIA; PREVOTELLA; PREVOTELLA NIGRESCENS;

EID: 0031939207     PISSN: 00039969     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-9969(97)00095-2     Document Type: Article

References (36)

1. Baumgartner J. C., Falkler W. A. Bacteria in the apical 5 mm of infected root canals. J. Endodontics. 17:1991;380-383.
2. Bedi G. S., Taffy W. Purification and characterization of a collagen-degrading protease from Porphyromonas gingivalis. J. Biol. Chem. 269:1994;599-606.
3. Fabricius L., Dahlen G., Ohman E., Moller A. J. Predominant endogenous oral bacteria isolated from infected root canals after varied times of closure. Scand. J. Dent. Res. 90:1982;134-144.
4. Fotos P. G., Lewis D. M., Gerencser V. F., Gerencser M. A. Cytotoxic and immunostimulatory effects of Bacteroides cell products. J. Oral Pathol. Med. 19:1990;360-366.
5. Gemmell E., Seymour G. J. Interleukin 1, interleukin 6 and transforming growth-factor β production by human gingival mononuclear cells following stimulation with Porphyromonas gigivalis and Fusobacterium nucleatum. J. Periodont. Res. 28:1993;122-129.
6. Gibbons R. J., MacDonald J. B. Degradation of collagenous substrates by Bacteroides melaninogenicus. J. Bacteriol. 81:1961;614-621.
7. Helgeland K., Nordby O. Cell cycle-specific growth inhibitory effect on human gingival fibroblasts by a toxin isolated from the culture medium of Actinobacillus actinomycetemcomitans. J. Periodont. Res. 28:1993;161-165.
8. Higerd T. B., Vesoole D. H., Goust J. M. Inhibitory effects of extracellular products from oral bacteria on human fibroblasts and stimulated lymphocytes. Infect. Immun. 21:1978;567-574.
9. Horiba N., Maekawa Y., Abe Y., Ito M., Matsumoto T., Nakamura H., Ozeki M. Cytotoxicity against various cell lines of lipopoly saccharides purified from Bacteroides fusobacterium and veillonella isolated from infected root canals. J. Endodontics. 15:1989;530-534.
10. Lino Y., Hopps R. M. The bone-resorbing activities in tissue culture of lipopolysaccharides from the bacteria Actinomyces actinomycetemcomitans, Bacteroides gingivalis and Capnocytophaga ochracea isolated from human mouths. Archs. Oral Biol. 29:1984;59-63.
11. Jansen H. J., Van der Hoeven J. S., Göertz J. H. C., Bakkeren. Breakdown of various serum proteins by periodontal bacteria. Microbial ecology in health and disease. 7:1994;299-305.
12. Jansen J. H., Van der Hoeven J. S., Van den Kieboom C. W. A., Göertz J. H. C., Camp P. J. M., Bakkeren. Degradation of immunoglobulin G by periodontal bacteria. Oral Microbiol. Immunol. 9:1994;345-351.
13. Jansen H. J., Grenier D., Van der Hoeven J. S. Characterisation of immunoglobulin G-degrading proteases of Prevotella intermedia and Prevotella nigrescens. Oral Microbiol. Immunol. 10:1995;138-145.
14. Jin K. C., Barua P. K., Zambon J. J., Neiders M. E. Proteolytic activity in black-pigmented Bacteroides species. J. Endodontics. 15:1989;463-467.
15. Laemli U. K. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature. 227:1970;680-685.
16. Larjava H., Vitto V., Eerola E., Haapasalo M. Inhibition of gingival fibroblast growth by Bacteroides gingivalis. Infect. Immun. 55:1987;201-205.
17. Layman D. L., Dietrich D. L. Growth inhibitory effects of endotoxin from bacteroides gingivalis and intermedius on human gingival cell cultures. J. Periodontol. 58:1987;387-392.
18. Loomer P. M., Sigysch B., Sukhu B., Ellen R. P., Tenenbaum H. C. Direct effects of metabolic products and sonicated extracts of Porphyromonas gingivalis 2561 on osteogenesis in vitro. Infection and Immunity. 62:1994;1289-1297.
19. Mayrand D., Grenier D. Detection of collagenase activity in oral bacteria. Can. J. Microbiol. 31:1985;134-138.
20. Robertson P. B., Lantz M., Marucha P. T., Kornman K. S., Trummel C. L., Holt S. C. Collagenolytic activity associated with Bacteroides species and Actino bacillus actinomycetemcomitans. J. Periodont. Res. 17:1982;275-283.
21. Sato M., Otsuka R., Mahara J., Endo J., Nakamura R. Degradation of human immunoglobulin A by protease isolated from the anaerobic periodontopathic bacterium Bacteroides gingivalis. Arch. Oral Biol. 32:1987;235-238.
22. Slots J., Genco R. J. Black-pigmented Bacteroides species, Capnocytophaga species, and Actinobacillus actinomycetemcomitans in human periodontal disease: virulence factors in colonization, survival and tissue destruction. J. Dent. Res. 63:1984;412-421.
23. Sugiyama E., Baehni P., Cimasoni G. An in vitro study of polymorphonuclear leucocyte-mediated injury to human gingival keratinocytes by periodontopathic bacterial extracts. Archs. Oral Biol. 37:1992;1007-1012.
24. Sundqvist G., Eckerbom M. I., Larson A. P., Sjögren U. T. Capacity of anaerobic bacteria from necrotic dental pulps to induce purulent infections. Infect. Immun. 25:1979;685-693.
25. Sundqvist G., Carlsson J., Herrmann B., Tarnvik A. Degradation of human immunoglobulin G and M and complement factors C3 and C5 by black-pigmented Bacteroides. J. Med. Microbiol. 19:1985;85-90.
26. Sundqvist G., Carisson J., Hanstrom L. Collagenolytic activity of black-pigmented Bacteroides species. J. Periodont. Res. 22:1987;300-306.
27. Sundqvist G. (1990) Endodontic microbiology. In Experimental Endodontics. (Eds. Spanberg L. S. W.), pp. 131-153. C.C.R. Press, Boca Raton, Florida.
28. Touw J. J., Van Steenbergen T. J. M., de Graaff J. Butyrate: a cytotoxin for vero cells produced by Bacteroides gingivalis and Bacteroides asaccharolyticus. Antonie Van Leeuwenhoek. 48:1982;315-325.
29. Touw J. J., Van Kampen G. P. J., Van Steenbergen T. M., Veldhuijzen J. P., de Graff J. The effects of culture filtrates of oral strains of black-pigmented Bacteroides on matrix production of chick embryo cartilage cells in vitro. J. Periodontol. Res. 17:1982;351-357.
30. Towbin H., Staehlin H., Gordon. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and applications. Proc. Natl. Acad. Sci. U.S.A. 76:1979;4350-4354.
31. Trowbridge H. Immunological aspects of chronic inflammation and repair. J. Endodontics. 16:1990;54-61.
32. Van Steenbergen T. J. M., de Graaff J. Proteolytic activity of black-pigmented Bacteroides strains. FEMS Microbiology letters. 33:1986;219-222.
33. Van Steenbergen T. J. M., Van Ouden M. D., Toow J. J. A., de Graaff J. Cytotoxic activity of Bacteroides gingivalis and Bacteroides asaccharolyticus. J. Med. Microbiol. 15:1982;253-258.
34. Van Strijp A. J. P., van Steenbergen T. J. M., de Graaff J., ten Cate J. M. Bacterial colonization and degradation of demineralized dentin matrix in situ. Caries Res. 28:1994;21-27.
35. Williams B. L., McCann G. F., Shoenknecht F. D. Bacteriology of dental abscesses of endodontic origin. J. Clin. Microbiol. 18:1983;770-774.
36. Yamasaki M., Nakane A., Kumazawa M., Hashioka K., Horiba N., Nakamura H. Endotoxin and gram negative bacteria in periapical lesions. J. Endodontics. 18:1992;501-504.