Small-angle X-ray scattering and computer-aided molecular modeling studies of 20 kDa fragment of porcine amelogenin: Does amelogenin adopt an elongated bundle structure?

Journal of Biochemistry

Volumn 123, Issue 1, 1998, Pages 150-156

  Matsushima, Norio ^a   Izumi, Yoshinobu ^b   Aoba, Takaaki ^c  

^a SAPPORO MEDICAL UNIVERSITY   (Japan)

^b YAMAGATA UNIVERSITY   (Japan)

^c NIPPON DENTAL UNIVERSITY   (Japan)

Author keywords

Elongated structure; Modeling; Porcine amelogenin; Small angle X ray scattering

Indexed keywords

AMELOGENIN; GLUTAMINE; HISTIDINE; LEUCINE; PROLINE;

ARTICLE; COMPUTER ANALYSIS; ENAMEL; MOLECULAR MODEL; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN STRUCTURE; SWINE; X RAY CRYSTALLOGRAPHY;

SUIDAE; SUS SCROFA;

EID: 0031938931     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021902     Document Type: Article

References (44)

1. Termine, J.D., Belcourt, A.B., Christner, P.J., Conn, K.M., and Nylen, M.V. (1980) Properties of dissociatively extracted fetal tooth matrix proteins. I. Principal molecular species in developing bovine enamel. J. Biol. Chem. 255, 9760-9768
2. Yamakoshi, Y., Tanabe, T., Fukae, M., and Shimizu, M. (1994) Porcine amelogenins. Calcif. Tissue Int. 54, 69-75
3. Fincham, A.G. and Moradian-Oldak, J. (1993) Amelogenin post-translational modifications: carboxy-terminal processing and the phosphorylation of bovine and porcine "TRAP" and "LRAP" amelogenins. Biochem. Biophys. Res. Commun. 197, 248-255
4. Snead, M.L., Lau, E.C., Zeichner-David, M., Fincham, A.G., Woo, S.L., and Slavkin, H.C. (1985) DNA sequence for cloned cDNA for murine amelogenin reveal the amino acid sequence for enamel-specific protein. Biochem. Biophys. Res. Commun. 129, 812-818
5. Lau, E.C., Simmer, J.P., Bringas, Jr, P., Hsu, D.D.J., Hu, C.-C., Zeichner-David, M., Thiemann, F., Snead, M.L., Slavkin, H.C., and Fincham, A.G. (1992) Alternative splicing of the mouse amelogenin primary RNA transcript contributes to amelogenin heterogeneity. Biochem. Biophys. Res. Commun. 188, 1253-1260
6. Shimokawa, H., Ogata, Y., Sasaki, S., Sobel, M.E., Mcquillan, C.I., Termine, J.D. and Young, M.F. (1987) Molecular cloning of bovine amelogenin cDNA. Adv. Dent. Res. 1, 293-297
7. Gibson, C., Golub, E., Herold, R., Risser, M., Ding, W., Shimokawa, H., Young, M., Termine, J.D., and Rosenbloom, J. (1991) Structure and expression of the bovine amelogenin gene. Biochemistry 30, 1075-1079
8. Takagi, T., Suzuki, M., Baba, T., Minegishi, K., and Sasaki, S. (1984) Complete amino acid sequence of amelogenin in developing bovine enamel. Biochem. Biophys. Res. Commun. 121, 592-597
9. Nakahori, Y., Takenaka, O., and Nakagome, Y. (1991) A human X-Y homologous region encodes amelogenin. Genomics 9, 264-269
10. Salido, E.C., Yen, P.H., Koprivnikar, K., Yu, L.C., and Shapiro, L.J. (1992) The human enamel protein gene amelogenin is expressed from both the X and the Y chromosomes [see comments]. Am. J. Human Genet. 50, 303-316
11. Fincham, A.G., Hu, Y., Pavlova, Z., Slavkin, H.C., and Snead, M.L. (1989) Human amelogenins: sequences of "TRAP" molecules. Calcif. Tissue Int. 45, 243-250
12. Bonass, W.A., Robinson, P.A., Kirkham, J., Shore, R.C., and Robinson, C. (1994) Molecular cloning and DNA sequence of rat amelogenin and a comparative analysis of mammalian amelogenin protein sequence divergence. Biochem. Biophys. Res. Commun. 198, 755-763
13. Hu, C.C., Zhang, C., Qian, Q., Ryu, H., Moradian-Oldak, J., Fincham, A.G., and Simmer, J.P. (1996) Cloning, DNA sequence, and alternative splicing of opossum amelogenin mRNAs. J. Dent. Res. 75, 1735-1741
14. Fincham, A.G., Lau, E.C., Simmer, J.P., and Zeichner-David, M. (1992) in Chemistry and Biology of Mineralized Tissues (Slavkin, H.C. and Price, P., eds.) pp. 187-201, Elsevier Science Publishers, Amsterdam
15. Goto, Y., Kogure, E., Takagi, T., Aimoto, S., and Aoba, T. (1993) Molecular conformation of porcine amelogenin in solution: three folding units at the N-terminal, central, and C-terminal regions. J. Biochem. 113, 55-60
16. Renugopalakrishnan, V., Strawich, E., Morowitz, P.M., and Glimcher, M.J. (1986) Studies of the secondary structures of amelogenin from bovine tooth enamel. Biochemistry 25, 4879-4887
17. Renugopalakrishnan, V., Pattabraman, N., Prabhakaran, M., Strawich, E., and Glimcher, M.J. (1989) Tooth enamel protein, amelogenin, has a probable beta-spiral internal channel, Gln112-Leu138, within a single polypeptide chain: preliminary molecular mechanics and dynamics studies. Biopolymers 28, 297-303
18. Zheng, S., Tu, A.T., Renugopalakrishnan, V., Strawich, E., and Glimcher, M.J. (1987) A mixed beta-turn and beta-sheet structure for bovine tooth enamel amelogenin: Raman spectroscopic evidence. Biopolymers 26, 1809-1813
19. Fukae, M., Ijiri, H., Tanabe, T., and Shimizu, M. (1979) Partial amino acid sequences of two proteins in developing porcine enamel. J. Dent. Res. 58, 1000-1001
20. Shimizu, M. and Fukae, M. (1983) in Mechanisms of Tooth Enamel Formation (Suga, S., ed.) pp. 125-141, Quintessence Publ., Tokyo
21. Aoba, T., Tanabe, T., and Moreno, E.C. (1987) Proteins in the enamel fluid of immature porcine teeth. J. Dent. Res. 66, 1721-1726
22. Aoba, T., Shimoda, S., Shimokawa, H., and Inage, T. (1992) Common epitopes of mammalian amelogenins at the C-terminus and possible functional roles of the corresponding domain in enamel mineralization. Calcif. Tissue Res. 51, 85-91
23. Aoba, T., Shimoda, S., Akita, H., Holmberg, C., and Taubman, M.A. (1992) Anti-peptide antibodies reactive with epitopic domains of porcine amelogenins at the C-terminus. Arch. Oral Biol. 37, 249-255
24. Tanabe, T., Fukae, M., and Shimizu, M. (1994) Degradation of enamelins by proteinases found in porcine secretory enamel in vitro. Arch. Oral Biol. 39, 277-281
25. Fincham, A.G., Moradian-Oldak, J., and Sarte, P.E. (1994) Mass-spectrographic analysis of a porcine amelogenin identifies a single phosphorylated locus. Calcif. Tissue Int. 55, 398-400
26. Ueki, T., Hiragi, Y., Izumi, Y., Tagawa, H., Kataoka, M., Muroga, Y., Matsushita, T., and Amemiya, Y. (1983) KEK Progress Reports 83-1, Photon Activity Report, 1982/1983, VI-70-VI-71
27. Glatter, O. (1982) Data treatment in Small Angle X-Ray Scattering (Glatter, O. and Kratky, O., eds.) pp. 119-196, Academic Press, London
28. Pilz, I. (1982) Proteins in Small Angle X-Ray Scattering (Glatter, O. and Kratky, O., eds.) pp. 239-293, Academic Press, London
29. Dempsey, S. (1987) Molecular Modeling System (MMS), Department of Chemistry Computer Facility, Univ. of California at San Diego
30. Tripos (1996) SYBYL6.3, Tripos Associates, St. Louis, MO
31. Matsushima, N., Danno, G., Sasaki, N., and Izumi, Y. (1992) Small-angle X-ray scattering study by synchrotron orbital radiation reveals that high molecular weight subunit of glutenin is a very anisotropic molecule. Biochem. Biophys. Res. Commun. 186, 1057-1064
32. Wellman, S.E., Hamodrakas, S.J., Kamitsos, E.I., and Case, S.T. (1992) Secondary structure of synthetic peptides derived from the repeating unit of a giant secretory protein from Chironomus tentans. Biochim. Biophys. Acta 1121, 279-285
33. Suzuki, S., Ishiwata, Y., Shimanouchi, T., and Tsuboi, M. (1966) Infrared spectra of isotopic polyglycines. Biopolymers 4, 337-350
34. Schultz, G.E. and Schirmer, R.H. (1979) in Principles of Protein Structure, pp. 66-107, Springer-Verlag, Berlin
35. Wilmot, C.M. and Thornton, J.M. (1988) Analysis and prediction of the different types of beta-turn in proteins. J. Mol. Biol. 203, 221-232
36. Efimov, A.V. (1993) Standard structures in proteins. Prog. Biophys. Mol. Biol. 60, 201-239
37. Strawich, E., Poon, P.H., Renugopalakrishnan, V., and Glimcher, M.J. (1985) Relative molecular mass determination of a major, highest relative molecular mass extracellular amelogenin of developing bovine enamel. FEBS Lett. 184, 188-192
38. Matsushima, N., Danno, G., Takezawa, H., and Izumi, Y. (1997) Three-dimensional structure of maize α-zein proteins studied by small-angle X-ray scattering. Biochim. Biophys. Acta 1339, 14-22
39. Kobe, B. and Deisenhofer, J. (1993) Crystal structure of porcine ribonuclease inhibitor, a protein with leucine-rich repeats. Nature 366, 751-756
40. Nikiforuk, G. and Simmons, N.S. (1965) Purification and properties of protein from embryonic bovine enamel. J. Dent. Res. 44, Suppl: 1119-1122
41. Moradian-Oldak, J., Simmer, J.P., Lau, E.C., Sarte, P.E., Slavkin, H.C., and Fincham, A.G. (1994) Detection of monodisperse aggregates of a recombinant amelogenin by 1 dynamic light scattering. Biopolymers 34, 1339-1347
42. Fincham, A.G., Moradian-Oldak, J., Diekwisch, T.G.H., Lyaruu, D.M., Wright, J.T., Bringas, Jr. P., and Slavkin, H.C. (1995) Evidence for amelogenin "nanospheres" as functional components of secretory-stage enamel matrix. J. Struct. Biol. 115, 50-59
43. Kraulis, P.J. (1991) MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures. J. Appl. Cryst. 24, 946-950
44. Guinier, A. and Fournet, G. (1955) in Small-Angle Scattering of X-Rays, p. 19, pp. 24-28, and pp. 135-136, Johns Wiley and Sons, New York