In situ polymerase chain reaction amplification of HIV-1 DNA in brain tissue

Journal of Virological Methods

Volumn 70, Issue 2, 1998, Pages 119-127

  Strappe, Pádraig M ^a   Wang, Ting Huei ^a   McKenzie, Chris Anne ^a   Lowrie, Suzanne ^a   Simmonds, Peter ^a   Bell, Jeanne E ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Brain tissue; Human immunodeficiency virus type 1; In situ PCR; Tyramide signal amplification

Indexed keywords

VIRUS DNA;

ARTICLE; AUTOPSY; BRAIN TISSUE; GENE AMPLIFICATION; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MICROWAVE IRRADIATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TISSUE SECTION;

BRAIN; DNA, VIRAL; FORMALDEHYDE; HIV-1; HUMANS; MICROWAVES; PARAFFIN EMBEDDING; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TISSUE FIXATION;

EID: 0031938923     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(97)00156-0     Document Type: Article

References (26)

1. Bagasra O., Hauptman S.P., Lischner H.W., Sachs M., Pomerantz R.J. Detection of human immunodeficiency virus type 1 provirus in mononuclear cells by in situ polymerase chain reaction. N. Eng. J. Med. 326:1992;1385-1391.
2. Bagasra O., Seshamma T., Pomerantz R.J. Polymerase chain reaction in situ: intracellular amplification and detection of HIV-1 proviral DNA and other specific genes. J. Immunol. Methods. 158:1993;131-145.
3. Bagasra O., Lavi E., Bobroski L., Khalalili K., Pestaner J.P., Tawadros R., Pomerantz R.J. Cellular reservoirs of HIV-1 in the central nervous system of infected individuals: identification by the combination of in situ polymerase chain reaction and immunohistochemistry. AIDS. 10:1996;573-585.
4. Berghorn K.A., Bonnett J.H., Hoffman G.E. cFos immunoreactivity is enhanced with biotin amplification. J. Histochem. Cytochem. 42:1994;1635-1642.
5. Budka H. Neuropathology of human immunodeficiency virus infection. Brain Pathol. 1:1991;163-175.
6. Donaldson Y.K., Bell J.E., Holmes E.C., Hughes E.S., Brown H.K., Simmonds P. In vivo distribution and cytopathology of variants of human immunodeficiency virus type 1 showing restricted sequence variability in the V3 loop. J. Virol. 68:1994;5991-6005.
7. Embretson J., Zupanic M., Beneke J., Till M., Wolinsky S., Ribas J.L., Burke A., Haase A.T. Analysis of human immunodeficiency virus-infected tissues by amplification and in situ hybridization reveals latent and permissive infections at single-cell resolution. Proc. Natl. Acad. Sci. USA. 90:1993;357-361.
8. Haase A.T., Retzel E.F., Staskus K.A. Amplification and detection of lentiviral DNA inside cells. Proc. Natl. Acad. Sci. USA. 87:1990;4971-4975.
9. Harrison, M.J.G., 1993. Neurological complications of HIV infection: clinical aspects. In: Scaravilli, F. (Ed.), The Neuropathology of HIV Infection. Springer Verlag, London, pp. 21-33.
10. Jackson D.P., Lewis F.A., Taylor G.R., Boylston A.W., Quirke P. Tissue extraction of DNA and RNA and analysis by the polymerase chain reaction. J. Clin. Pathol. 43:1990;499-504.
11. Kerstens H.M.J., Poddighe P.J., Hanselaar A.G.J.M. A novel in situ hybridization signal amplification method based on the deposition of biotinylated tyramine. J. Histochem. Cytochem. 43:1995;347-352.
12. Komminoth P., Long A.A., Ray R., Wolfe H.J. In situ PCR detection of viral DNA, single copy genes and gene arrangements in cell suspensions and cytospins. Diag. Mol. Pathol. 1:1992;85-97.
13. Kuo-Ping C., Cohen S.T., Morris D.W., Jordan G.W. Intracellular amplification of proviral DNA in tissue sections using the polymerase chain reaction. J. Histochem. Cytochem. 40:1992;333-341.
14. Lewis F.A. In situ PCR - myth or magic? J. Cell. Pathol. 1:1996;13-23.
15. Long A.A., Komminoth P., Lee E., Wolf H.K. Comparision of indirect and direct in situ polymerase chain reaction in cell preparations and tissue sections. Histochemistry. 99:1993;151-162.
16. McQuaid S., McConnell R., McMahon J., Herron B. Microwave antigen retrieval for immunocytochemistry on formalin-fixed, paraffin-embedded post-mortem CNS tissue. J. Pathol. 176:1995;207-216.
17. Nuovo G.J., Forde A., MacConell P., Fahrenwald R. In situ detection of HIV-1 nucleic acids and tumour necrosis factor cDNA in cervical tissues. Am. J. Pathol. 143:1993;40-48.
18. Nuovo G.J., MacConnell P., Gallery F. Analysis of nonspecific DNA synthesis during In Situ PCR and solution-phase PCR. PCR Methods Appl. 4:1994;89-96.
19. Nuovo G.J., Gallery F., MacConell P., Braun A. In Situ detection of polymerase chain reaction-amplified HIV-1 nucleic acids and tumour necrosis factor-alpha in the central nervous system. Am. J. Pathol. 144:1994;659-666.
20. Patterson B.K., Till M., Otto P., Goolsby C., Furtado M.R., McBride L.J., Wolinsky S.M. Detection of HIV-1 DNA and messenger RNA in individual cells by PCR-driven in situ hybridization and flow cytometry. Science. 260:1993;976-979.
21. Ranki A., Nyberg M., Ovod V., Haltia M., Elovaar I., Raininko R., Haapasalo H., Krohn K. Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS. 9:1995;1001-1008.
22. Saito Y., Sharer L.R., Epstein L.G., Michaels J., Mintz M., Louder M., Golding K., Cvetkovich T.A., Blumberg B.M. Overexpression of NEF as a marker for restricted HIV-1 infection of astrocytes in post mortem paediatric central nervous tissues. Neurology. 44:1994;474-481.
23. Takahashi K., Wesselingh S.L., Griffin D.E., McArthur J.C., Johnson R.T., Glass J.D. Localization of HIV-1 in human brain using polymerase chain reaction/in situ hybridisation and immunocytochemistry. Ann Neurol. 39:1996;705-711.
24. Teo I.A., Shaunak S. PCR in situ: aspects which reduce amplification and generate false-positive results. Histochem. J. 27:1995;660-669.
25. Tokusashi Y., Nishikawa Y., Katsuhiro O. Differentiation of the normal and mutant rat albumin genes on hepatic tissue sections by in situ PCR. Nucleic Acids Res. 23:1995;3790-3791.
26. Wiley C.A., Schrier R.D., Nelson J.A., Lampet P.W., Oldstone M.B.A. Cellular localisation of human immunodeficiency virus infection within brains of acquired immunodeficiency syndrome patients. Proc. Natl. Acad. USA. 83:1986;7089-7093.