Study of amide proton exchange in 15N-enriched cryptogein using pH- dependent off-resonance ROESY-HSQC experiments

European Biophysics Journal

Volumn 27, Issue 2, 1998, Pages 167-171

  Fefeu, Sandrine ^a   Birlirakis, Nicolas ^a   Guittet, Eric ^a  

^a INSTITUT DE CHIMIE DES SUBSTANCES NATURELLES   (France)

Author keywords

Cryptogein; Elicitin; Off resonance ROESY; Water protein exchange

Indexed keywords

AMIDE; HYDROGEN; NITROGEN 15; PROTEIN; PROTON; SOLVENT; WATER;

ARTICLE; BINDING SITE; NUCLEAR OVERHAUSER EFFECT; PH;

ALGAL PROTEINS; AMIDES; FUNGAL PROTEINS; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTONS; SOLVENTS; WATER;

EID: 0031938793     PISSN: 01757571     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002490050122     Document Type: Article

References (24)

1. Birlirakis N, Cerdan R, Guittet E (1996) A study of protein-water exchange through the off-resonance ROESY experiment: application to the DNA-binding domain of Alcr. J Biomol NMR 8:487-491
2. Desvaux H, Berthault P, Birlirakis N, Goldman M (1994) Off-resonance ROESY for the study of dynamic processes. J Magn Reson A108:219-229
3. Desvaux H, Berthault P, Birlirakis N, Goldman M, Piotto M (1995a) Improved versions of off-resonance ROESY. J Magn Reson A113:47-52
4. 1H NMR relaxation measurements. Chem Phys Lett 233:545-549
5. Englander SW, Kallenbach NR(1984) Hydrogen exchange and structural dynamics of proteins and nucleic acids. Q Rev Biophys 16:521-655
6. Englander JJ, Englander SW, Louie G, Roder H, Tran T, Wand AJ (1988) Protein hydrogen exchange, dynamics, and energetics. In: Structure and expression 1, Adanine Press, pp 107-117
7. Englander SW, Sosnick TR, Englander JJ, Mayne L (1996) Mechanisms and uses of hydrogen exchange. Curr Opin Struct Biol 6:18-23
8. Fefeu S, Bouaziz S, Huet J-C, Pernollet J-C, Guittet E (1997) Three-dimensional solution structure of β cryptogein, a β elicitin secreted by phytophthora cryptogea. Protein Science, 6: 2279-2284
9. 15N-enriched calcineurin B. J Biomol NMR 3:627-638
10. Hwang T-L, Mori S, Shaka AJ, van Zijl PCM (1997) Application of phase-modulated CLEAN chemical exchange spectroscopy (CLEANEX-PM) to detect water-protein proton exchange and intermolecular NOEs. J Am Chem Soc 119:6203-6204
11. Kim K-S, Fuchs JA, Woodward CK (1993) Hydrogen exchange identifies native-state motional domains important in protein folding. Biochemistry 32:9600-9608
12. Macura S, Wüthrich K, Ernst RR (1982) Separation and suppression of coherent transfer effects in two dimensional NOE and chemical exchange spectroscopy. J Magn Reson 46:269-282
13. Moonen CTW, van Gelderen P, Vuister GW, van Zijl PCM (1992) Gradient-enhanced exchange spectroscopy. J Magn Reson 97: 419-425
14. Mori S, Johnson MO, Berg JM, Van Zijl PCM (1994) Water exchange filter (WEX filter) for nuclear magnetic resonance studies of macromolecules. J Am Chem Soc 116:11982-11984
15. Mori S, Abeygunawardana C, van Zijl PCM, Berg JM (1996) Water exchange filter with improved sensitivity (WEX II) to study solvent-exchangeable protons. Application to the consensus zinc finger peptide CP-1. J Magn Reson B 110:96-101
16. O'Donohue MJ, Gousseau H, Huet J-C, Tepfer D, Pernollet J-C (1995) Chemical synthesis, expression and mutagenesis of a gene encoding β-cryptogein, an elicitin produced by Phytophthora cryptogea. Plant Mol Biol 27:577-586
17. Otting G, Wüthrich K (1989) Studies of protein hydration in aqueous solution by direct NMR observation of individual protein-bound water molecules. J Am Chem Soc 111:1871-1875
18. Pernollet J-C, Sallantin M, Sallé-Tourne M, Huet J-C (1993) Elicitin isoforms from seven Phyophthora species: comparison of their physico-chemical properties and toxicity to tobacco and other plant species. Physiol Mol Plant Pathol 42:53-67
19. Pons JL, Malliavin TE, Delsuc MA (1996) A complete package for NMR data and set processing. J Biomol NMR 8:445-452
20. Rothemund S, Beyermann M, Krause E, Krause G, Bienert M, Hodges RS, Sykes BD, Sönnichen FD (1995) Structure effects of double D-amino acid replacements: a nuclear magnetic resonance and circular dichroism study using amphipathic model helices. Biochemistry 34:12954-12962
21. Roumestand C, Mispelter J, Austry C, Canet D (1995) The use of band filtering in multidimensional NMR. Evaluation of two "user-friendly" techniques. J Magn Reson B109: 153-163
22. Tercé-Laforgue T, Huet J-C, Pernollet J-C (1992) Biosynthesis and secretion of cryptogein, a protein elicitor secreted by Phytophthora cryptogea. Plant Physiol 98:936-941
23. Wider G, Riek R, Wüthrich K (1996) Diffusion filters for separation of solvent-protein and protein-protein nuclear overhauser effects (HYDRA). J Am Chem Soc 118:11629-11634
24. Wüthrich K (1986) NMR of proteins and nuclear acids. Wiley, New York