Use of T4 DNA polymerase to create cohesive termini in PCR products for subcloning and site-directed mutagenesis

BioTechniques

Volumn 24, Issue 1, 1998, Pages 30-34

  Cooney, Austin J ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE; DEOXYADENOSINE; DNA; DNA FRAGMENT; DNA POLYMERASE; EXONUCLEASE; GUANOSINE; NUCLEOTIDE; OLIGONUCLEOTIDE; OVALBUMIN; RESTRICTION ENDONUCLEASE; TRANSCRIPTION FACTOR;

ARTICLE; BINDING AFFINITY; CLONING; DNA BINDING; LONG TERMINAL REPEAT; NONHUMAN; POINT MUTATION; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS;

EID: 0031938424     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98241bm04     Document Type: Article

References (7)

1. Cooney, A.J., S.V. Tsai, B.W. O'Malley and M.-J. Tsai. 1991. Chicken ovalhumin upstream promoter transcription factor binds to a negative regulatory region in the human immunodeficiency virus type 1 long terminal repeat. J. Virol. 65:2853-2860.
2. Hemsley, A., N. Arnheim, M.D. Toney, G. Cortopassi and D.J. Galas. 1989. A simple method for site-directed mutagenesis using the polymerase chain reaction. Nucleic Acids Res. 17:6545-6551.
3. Innis, M.A., D.H. Gelfand, J.J. Sninsky and T.J. White. 1990. PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego.
4. Mead, D.A., N. Kristy, C. Hemstadt, R.A. Maril and M.S. Lloyd. 1991. A universal method for the direct cloning of PCR amplified nucleic acid. Bio/Technology 9:657-663.
5. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
6. Sanger, F., S. Nicklen and A.R. Coulson. 1977. DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
7. Sodroski, J., R. Partarca, C. Rosen, F. Wong-Staal and W. Haseltine. 1986. Location of the trans-activating region on the genome of human T-cell lymphotropic virus type III. Science 227:538-540.