Requirement for an aromatic amino acid or histidine at the N terminus of sindbis virus RNA polymerase

Journal of Virology

Volumn 72, Issue 3, 1998, Pages 2310-2315

  Shirako, Yukio ^a,b   Strauss, James H ^a  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AROMATIC AMINO ACID; HISTIDINE; PHENYLALANINE; RNA POLYMERASE; TRYPTOPHAN; TYROSINE;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CHICKEN; EMBRYO; ENZYME STRUCTURE; MUTANT; NONHUMAN; PRIORITY JOURNAL; RNA CLEAVAGE; RNA REPLICATION; SINDBIS VIRUS;

EID: 0031937629     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.72.3.2310-2315.1998     Document Type: Article

References (23)

1. Barton, D. J., S. G. Sawicki, and D. L. Sawicki. 1988. Demonstration in vivo of temperature-sensitive elongation of RNA in Sindbis virus mutant ts6. J. Virol. 62:3597-3602.
2. Barton, D. J., S. G. Sawicki, and D. L. Sawicki. 1991. Solubilization and immunoprecipitation of alphavirus replication complexes. J. Virol. 65:1496-1506.
3. Chamberlain, J. P. 1979. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal. Biochem. 98:132-135.
4. de Groot, R. J., W. R. Hardy, Y. Shirako, and J. H. Strauss. 1990. Cleavage-site preferences of Sindbis virus polyproteins containing the nonstructural proteinase: evidence for temporal regulation of polyprotein processing in vivo. EMBO J. 9:2631-2638.
5. de Groot, R. J., T. Rümenapf, R. J. Kuhn, E. G. Strauss, and J. H. Strauss. 1991. Sindbis virus RNA polymerase is degraded by the N-end rule pathway. Proc. Natl. Acad. Sci. USA 88:8967-8971.
6. Gmyl, A. P., E. V. Pilipenko, S. V. Maslova, G. A. Belov, and V. I. Agol. 1993. Functional and genetic plasticities of the poliovirus genome: quasi-infectious RNAs modified in the 5′-untranslated region yield a variety of pseudorevertants. J. Virol. 67:6309-6316.
7. Gubler, U., and B. J. Hoffman. 1983. A simple and very efficient method for generating cDNA libraries. Gene 25:263-269.
8. - temperature-sensitive mutants of Sindbis virus: complementation group F mutants have lesions in nsP4. J. Virol. 63:1194-1202.
9. Hardy, W. R., and J. H. Strauss. 1988. Processing of the nonstructural polyproteins of Sindbis virus: study of the kinetics in vivo using monospecific antibodies. J. Virol. 62:998-1007.
10. Hardy, W. R., and J. H. Strauss. 1989. Processing the nonstructural polyproteins of Sindbis virus: nonstructural proteinase is in the C-terminal half of nsP2 and functions both in cis and in trans. J. Virol. 63:4653-4664.
11. Holmes, D. S., and M. Quigley. 1981. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 114:193-197.
12. Kamer, G., and P. Argos. 1984. Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses. Nucleic Acids Res. 12:7269-7282.
13. Koonin, E. V. 1991. The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses. J. Gen. Virol. 72:2197-2206.
14. Lemm, J. A., and C. M. Rice. 1993. Assembly of functional Sindbis virus RNA replication complexes: requirement for coexpression of P123 and P34. J. Virol. 67:1905-1915.
15. Lemm, J. A., and C. M. Rice. 1993. Roles of nonstructural polyproteins and cleavage products in regulating Sindbis virus RNA replication and transcription. J. Virol. 67:1916-1926.
16. Lemm, J. A., T. Rümenapf, E. G. Strauss, J. H. Strauss, and C. M. Rice. 1994. Polypeptide requirements for assembly of functional Sindbis virus replication complexes: a model for the temporal regulation of minus-strand and plus-strand RNA-synthesis. EMBO J. 13:2925-2934.
17. Li, G., and C. M. Rice. 1989. Mutagenesis of the in-frame opal termination codon preceding nsP4 of Sindbis virus: studies of translational readthrough and its effect on virus replication. J. Virol. 63:1326-1337.
18. Rice, C. M., R. Levis, J. H. Strauss, and H. V. Huang. 1987. Production of infectious RNA transcripts from Sindbis virus cDNA clones: mapping of lethal mutations, rescue of a temperature-sensitive marker, and in vitro mutagenesis to generate defined mutants. J. Virol. 61:3809-3819.
19. Shirako, Y., and J. H. Strauss. 1990. Cleavage between nsP1 and nsP2 initiates the processing pathway of Sindbis virus nonstructural polyprotein P123. Virology 177:54-64.
20. Shirako, Y., and J. H. Strauss. 1994. Regulation of Sindbis virus RNA replication: uncleaved P123 and nsP4 function in minus-strand RNA synthesis whereas cleaved products from P123 are required for efficient plus-strand RNA synthesis. J. Virol. 68:1874-1885.
21. Strauss, E. G., C. M. Rice, and J. H. Strauss. 1983. Sequence coding for the alphavirus nonstructural proteins is interrupted by an opal termination codon. Proc. Natl. Acad. Sci. USA 80:5271-5275.
22. Strauss, J. H., and E. G. Strauss. 1994. The alphaviruses: gene expression, replication, and evolution. Microbiol. Rev. 58:491-562.
23. Varshavsky, A. 1992. The N-end rule. Cell 69:725-735.