Impact of 3'-exonuclease stereoselectivity on the kinetics of phosphorothioate oligonucleotide metabolism

Antisense and Nucleic Acid Drug Development

Volumn 8, Issue 1, 1998, Pages 35-42

  Gilar, Martin ^a   Belenky, Alexei ^a   Budman, Yeva ^a   Smisek, David L ^a   Cohen, Aharon S ^a  

^a HYBRIDON INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; EXONUCLEASE; OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATE; TETRAMER;

ARTICLE; CHIRALITY; DIASTEREOISOMER; HUMAN; KINETICS; MOLECULAR INTERACTION; NUCLEOTIDE METABOLISM; PRIORITY JOURNAL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; STEREOCHEMISTRY;

EID: 0031936347     PISSN: 10872906     EISSN: None     Source Type: Journal    
DOI: 10.1089/oli.1.1998.8.35     Document Type: Article

References (7)

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2. ALMER, H., STAWINSKI, J., and STROMBERG, R. (1996). Solid support synthesis of all-Rp-oligo(ribonucleoside phosphorothioate)s. Nucleic Acids Res. 24, 3811-3820.
3. BRUIN, G.J.M., BORNSEN, K.O., HUSKEN, D., GASSMANN E., WIDMER, M.H., and PAULUS, A. (1995). Stability measurements of antisense oligonucleotides by capillary gel electrophoresis. J. Chromatogr. 709, 181-195.
4. BRYANT, F.R., and BENKOVIC, S.J. (1979). Stereochemical course of the reaction catalyzed by 5′-nucleotide phosphodiesterase from snake venom. Biochemistry 18, 2825-2828.
5. BURGERS, P.M.J., and ECKSTEIN, F. (1978). Absolute configuration of the diastereomers of adenosine 5′-O-(1-thiotriphosphate): Consequences for the stereochemistry of polymerization by DNA-dependent RNA polymerase from Escherichia coli. Proc. Natl. Acad. Sci. USA 75, 4798-4800.
6. BURGERS, P.M.J., and ECKSTEIN, F. (1979). Stereochemistry of internucleotide bond formation by polynucleotide phosphorylase from Micrococcus luteus. Biochemistry 18, 450-454.
7. BURGERS, P.M.J., SATHYANARAYANA, B.K., SAENGER, W., and ECKSTEIN, F. (1979). Crystal and molecular structure of adenosine 5′-O-phosphorothioate O-p-nitrophenyl ester (sp diastereomer). Substrate stereospecificity of snake venom phosphodiesterase. Eur. J. Biochem. 100, 585-591.