Genetic analysis, using P22 challenge phage, of the nitrogen activator protein DNA-binding site in the Klebsiella aerogenes put operon

Journal of Bacteriology

Volumn 180, Issue 3, 1998, Pages 571-577

  Chen, Li Mei ^a,d   Goss, Thomas J ^b   Bender, Robert A ^b   Swift, Simon ^c   Maloy, Stanley ^a,c  

^a UNIVERSITY OF MICHIGAN   (United States)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^c UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^d STONY BROOK UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; DNA BINDING PROTEIN; MUTANT PROTEIN; REGULATOR PROTEIN;

ARTICLE; BACTERIAL GENE; BACTERIOPHAGE; ESCHERICHIA COLI; GENE MUTATION; GENETIC ANALYSIS; KLEBSIELLA AEROGENES; NONHUMAN; OPERON; PRIORITY JOURNAL; PROTEIN ANALYSIS; SALMONELLA TYPHIMURIUM;

EID: 0031935121     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.3.571-577.1998     Document Type: Article

References (28)

1. Bender, R. A. 1991. The role of the NAC protein in the nitrogen regulation of Klebsiella aerogenes. Mol. Microbiol. 5:2575-2580.
2. Benson, N., P. Sugiono, S. Bass, L. Mendelman, and P. Youderian. 1986. General selection for specific DNA-binding activities. Genetics 114:1-14.
3. Best, E., and R. Bender. 1990. Cloning of the Klebsiella aerogenes nac gene, which encodes a factor required for nitrogen regulation of the histidine utilization (hut) operons in Salmonella typhimurium. J. Bacteriol. 172:7043-7048.
4. Chen, L.-M., and S. Maloy. 1991. Regulation of proline utilization in enteric bacteria: cloning and characterization of the Klebsiella put control region. J. Bacteriol. 173:783-790.
5. Chow, W., and D. Berg. 1988. Tn5tac1, a derivative of transposon Tn5 that generates conditional mutants. Proc. Natl. Acad. Sci. USA 85:6468-6472.
6. Davis, R., D. Botstein, and J. Roth. 1980. Advanced bacterial genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
7. Garner, M., and A. Rezin. 1981. A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: applications to components of the E. coli lactose operon regulatory system. Nucleic Acids Res. 9:3047-3060.
8. Goss, T. J., and R. A. Bender. 1995. The nitrogen assimilation control protein, NAC, is a DNA binding transcription activator in Klebsiella aerogenes. J. Bacteriol. 177:3546-3555.
9. Graña, D., P. Youderian, and M. Susskind. 1985. The effects of mutations in the ant promoter of Salmonella phage P22. Genetics 110:1-6.
10. Hahn, D., R. Myers, C. Kent, and S. Maloy. 1988. Regulation of proline utilization in Salmonella typhimurium: molecular characterization of the put operon and DNA sequence of the put control region. Mol. Gen. Genet. 213:125-133.
11. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
12. Hughes, K. T., P. Youderian, and M. Simon. 1988. Phase variation in Salmonella: analysis of Hin recombinase and hix recombination site interaction in vivo. Genes Dev. 2:937-948.
13. Maloy, S. 1987. The proline utilization operon p. 1513-1519. In F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology, vol 2. American Society for Microbiology, Washington, D.C.
14. Maloy, S. 1989. Experimental techniques in bacterial genetics. Jones and Bartlett, Boston, Mass.
15. Maloy, S., V. Stewart, and R. Taylor. 1996. Genetic analysis of pathogenic bacteria: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
16. Maloy, S., and P. Youderian. 1993. Challenge phage: a genetic tool kit for dissecting DNA-protein interactions in vivo. Methods Mol. Genet. 3:205-233.
17. McCabe, P. 1990. Production of single-stranded DNA by asymmetric PCR, p. 76-83. In M. A. Innis, D. H. Gelfand, J. J. Snibsky, and T. J. White (ed.), PCR protocols: a guide to methods and Applications. Academic Press, New York, N.Y.
18. Mead, D., E. Szczesna-Sorupa, and B. Kemper. 1986. Single-stranded DNA 'Blue' T7 promoter plasmids: a versitile tandem promoter system for cloning and protein engineering. Protein Eng. 1:67-74.
19. Ostrovsky de Spicer, P., K. O'Brien, and S. Maloy. 1991. Regulation of proline utilization in Salmonella typhimurium: a membrane-associated dehydrogenase binds DNA in vitro. J. Bacteriol. 173:211-219.
20. Perry, K., and G. Walker. 1982. Identification of plasmid (pKM101)-coded proteins involved in mutagenesis and UV resistance. Nature 300:278-281.
21. 20a. Pomposiello, P. J., B. K. Janes, and R. A. Bender. Two roles for the DNA recognition site of Klebsiella aerogenes Nitrogen assimilation control protein. J. Bacteriol. 180:578-585.
22. Prival, M., and B. Magasanik. 1971. Resistance to catabolite repression of histidase and proline oxidase during nitrogen-limited growth of Klebsiella aerogenes. J. Biol. Chem. 246:6288-6296.
23. Sanderson, K., and J. Roth. 1988. Linkage map of Salmonella typhimurium, edition VII. Microbiol. Rev. 52:485-532.
24. Schwacha, A., and R. A. Bender. 1993. The product of the Klebsiella aerogenes nac (nitrogen assimilation control) gene is sufficient for activation of the hut operons and repression of the gdh operon. J. Bacteriol. 175:2116-2124.
25. Schwacha, A., and R. A. Bender. 1993. The nac (nitrogen assimilation control) gene from Klebsiella aerogenes. J. Bacteriol. 175:2107-2115.
26. Silhavy, T., L. Enquist, and M. Berman. 1984. Experiments with gene fusions. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
27. Wood, J. 1981. Genetics of L-proline utilization in Escherichia coli. J. Bacteriol. 146:895-901.
28. Youderian, P., A. Vershone, S. Bouvier, R. Sauer, and M. Susskind. 1983. Changing the DNA-binding specificity of a repressor. Cell 35:777-783.