Cloning, sequence analysis and expression in E. coli of the DNA polymerase I gene from Chloroflexus aurantiacus, a green nonsulfur eubacterium

Genetic Analysis - Biomolecular Engineering

Volumn 14, Issue 3, 1998, Pages 75-83

  Tvermyr, Marianne ^a,b   Kristiansen, Bjørn E ^a   Kristensen, Tom ^a  

^a UNIVERSITY OF OSLO   (Norway)

^b OSLO UNIVERSITY HOSPITAL   (Norway)

Author keywords

3' 5' exonuclease; 3' 5' exonuclease assay; 5' 3' exonuclease; DNA polymerase; Evolution; Phylogeny; polA

Indexed keywords

BACTERIAL ENZYME; DNA DIRECTED DNA POLYMERASE BETA;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; ESCHERICHIA COLI; EUBACTERIUM; GENE EXPRESSION; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PHYLOGENY; PRIORITY JOURNAL; PROTEIN DOMAIN; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; CHLOROBI; CLONING, MOLECULAR; DNA POLYMERASE I; ESCHERICHIA COLI; EXODEOXYRIBONUCLEASES; GENE EXPRESSION; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; PHYLOGENY; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0031934522     PISSN: 10503862     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1050-3862(97)10002-X     Document Type: Article

References (28)

1. [1] Braithwaite DK, Ito J. Nucleic Acids Res 1993;21:787-802.
2. [2] Joyce CM, Steitz TA. Annu Rev Biochem 1994;63:777-822.
3. [3] Klenow H, Henningsen I. Proc Natl Acad Sci USA 1970;65:168-75.
4. [4] Tabor S, Richardson CC. Proc Natl Acad Sci USA 1995;92:6339-43.
5. [5] Pierson BK, Castenholz RW. Arch Microbiol 1974;100:5-24.
6. [6] Chen W, Kuo T. Nucleic Acids Res 1993;21:2260.
7. [7] Sambrook J, Fritsch EF, Maniatis T, editors. Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory, 1989:1.29-1.30/2.118-2.120.
8. [8] Liu Z, Mishra NC. BioTechniques 1995;18:214-7.
9. [9] Inoue H, Nojima H, Okayama H. Gene 1990;96:23-8.
10. [10] Pluthero FG. Nucleic Acids Res 1993;21:4850-1.
11. [11] Thompson JD, Higgins DG, Gibson TJ. Nucleic Acids Res 1994;22:4673-80.
12. [12] Beese LS, Derbyshire V, Steitz TA. Science 1993;260:352-5.
13. [13] Korolev S, Nayal M, Barnes WM, Di Cera E, Waksman G. Proc Natl Acad Sci USA 1995;92:9264-8.
14. [14] Holm L, Sander C. J Mol Biol 1993;233:123-38.
15. [15] Nicolas KB, Nicolas HB Jr. GeneDoc: a tool for editing and annotating multiple sequence alignments. Distributed by the author, 1996.
16. [16] Felsenstein J. PHYLIP (Phylogeny Inference Package) version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle, 1993.
17. [17] Page RDM. Comput Appl Biosci 1996;12:357-8.
18. [18] Uemori T, Ishino Y, Fujita K, Asada K, Kato I. J Biochem (Tokyo) 1993;113:401-10.
19. [19] Gutman PD, Minton KW. Nucl Acids Res 1993;21:4406-7.
20. [20] Derbyshire V, Grindley NDF, Joyce CM. Eur Mol Biol Organ J 1991;10:17-24.
21. [21] Bernad A, Blanco L, Lazaro JM, Martin G, Salas M. Cell 1989;59:219-28.
22. [22] Delarue M, Poch O, Tordo N, Moras D, Argos P. Protein Eng 1990;3:461-7.
23. [23] Maidak BL, Olsen GJ, Larsen N, Overbeek R, McCaughey MJ, Woese CR. Nucleic Acids Res 1996;24:82-5.
24. [24] Huang MM, Arnheim N, Goodman MF. Nucleic Acids Res 1992;20:4567-73.
25. [25] Eckert K, Kunkel TA. Nucleic Acids Res 1990;18:3739-44.
26. [26] Aliotta JM, Pelletier JJ, Ware JL, Moran LS, Benner JS, Kong H. Genet Anal Biomol Eng 1996;12:185-95.
27. [27] Diaz A, Pons ME, Lacks SA, Lopez P. J Bacteriol 1992;174:2014-24.
28. [28] Brook TD, Madigan MT. Biology of Microorganisms, 6th ed. Prentice-Hall, Englewood Cliffs, NJ, 1991.