Use of a two-color genetic screen to identify a domain of the global regulator Lrp that is specifically required for pap phase variation

Journal of Bacteriology

Volumn 180, Issue 5, 1998, Pages 1224-1231

  Kaltenbach, Linda ^a   Braaten, Bruce ^a   Tucker, Julie ^a   Krabbe, Margareta ^a   Low, David ^a  

^a UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BACTERIUM PROTEIN PAP; REGULATOR PROTEIN; REGULATOR PROTEIN LRP; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIUM MUTANT; BACTERIUM PILUS; ESCHERICHIA COLI; GENETIC SCREENING; GENETIC VARIABILITY; NONHUMAN; PRIORITY JOURNAL; REGULON; TRANSACTIVATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0031934210     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.5.1224-1231.1998     Document Type: Article

References (19)

1. Blyn, L. B., B. A. Braaten, C. A. White-Ziegler, D. H. Rolfson, and D. A. Low. 1989. Phase-variation of pyelonephritis-associated pili in Escherichia coli: evidence for transcriptional regulation. EMBO J. 8:613-620.
2. Braaten, B. A., L. B. Blyn, B. S. Skinner, and D. A. Low. 1991. Evidence for a methylation-blocking factor (mbf) locus involved in pap pilus expression and phase variation in Escherichia coli. J. Bacteriol. 173:1789-1800.
3. Braaten, B. A., X. Nou, L. S. Kaltenbach, and D. A. Low. 1994. Methylation patterns in pap regulatory DNA control pyelonephritis-associated pili phase variation in E. coli. Cell 76:577-588.
4. Braaten, B. A., J. V. Platko, M. W. van der Woude, B. H. Simons, F. K. der Graaf, J. M. Calvo, and D. A. Low. 1992. Leucine-responsive regulatory protein controls the expression of both the pap and fan pili operons in Escherichia coli. Proc. Natl. Acad. Sci. USA 89:4250-4254.
5. Calvo, J. M., and R. G. Matthews. 1994. The leucine-responsive regulatory protein (Lrp), a global regulator of metabolism in Escherichia coli. Microbiol. Rev. 58:466-490.
6. Chang, A. C., and S. Cohen. 1978. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134:1141-1158.
7. Goransson, M., P. Forsman, P. Nilsson, and B. E. Uhlin. 1989. Upstream activating sequences that are shared by two divergently transcribed operons mediate cAMP-CRP regulation of pilus-adhesin in Escherichia coli. Mol. Microbiol. 3:1557-1565.
8. Kaltenbach, L. S., B. A. Braaten, and D. A. Low. 1995. Specific binding of PapI to Lrp-pap DNA complexes. J. Bacteriol. 177:6449-6455.
9. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
10. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
11. Nou, X., B. A. Braaten, L. S. Kaltenbach, and D. A. Low. 1995. Differential binding of Lrp to two sets of pap DNA binding sites mediated by PapI regulates Pap phase variation in Escherichia coli. EMBO J. 14:5785-5795.
12. Nou, X., B. Skinner, B. Braaten, L. Blyn, D. Hirsh, and D. Low. 1993. Regulation of pyelonephritis-associated pili phase variation in Escherichia coli: binding of the PapI and Lrp regulatory proteins is controlled by DNA methylation. Mol. Microbiol. 7:545-553.
13. Platko, J. V., and J. M. Calvo. 1993. Mutations affecting the ability of Escherichia coli Lrp to bind DNA. activate transcription, or respond to leucine. J. Bacteriol. 175:1110-1117.
14. Platko, J. V., D. A. Willins, and J. M. Calvo. 1990. The ilvIH operon of Escherichia coli is positively regulated. J. Bacteriol. 172:4563-4570.
15. Simons, R. W., F. Houman, and N. Kleckner. 1987. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53: 85-96.
16. van der Woude, M., B. Braaten, and D. Low. 1996. Epigenetic phase variation of the pap operon in Escherichia coli. Trends Microbiol. 4:5-9.
17. van der Woude, M., L. S. Kaltenbach, and D. A. Low. 1995. Leucine-responsive regulatory protein plays dual roles as both an activator and a repressor of the Escherichia coli pap operon. Mol. Microbiol. 17:303-312.
18. Wang, Q., and J. M. Calvo. 1993. Lrp, a global regulatory protein of Escherichia coli, binds co-operatively to multiple sites and activates transcription of ilvIH. J. Mol. Biol. 229:306-318.
19. Zhou, Y., X. Zhang, and R. H. Ebright. 1993. Identification of the activating region of catabolite gene activator protein (CAP): isolation and characterization of mutants of CAP specifically defective in transcription activation. Proc. Natl. Acad. Sci. USA 90:6081-6085.