Gene control in somatic embryos of Digitalis lanata: Expression of the ß-glucuronidase gene fused to a plastocyanin promoter

Botanica Acta

Volumn 111, Issue 1, 1998, Pages 22-27

  Thomar, S ^a   Dumke Lehmann, Ulrike ^a   Diettrich, Beate ^a   Luckner, M ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Digitalis lanata; Plastocyanin promoter; Somatic embryogenesis; Transformation; glucuronidase gene

Indexed keywords

DIGITALIS LANATA;

EID: 0031933267     PISSN: 09328629     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1438-8677.1998.tb00672.x     Document Type: Article

References (40)

1. An, G. - Binary Ti vectors for plant transformation and promoter analysis. Meth. Enzymology 153 (1987), 292-305.
2. Bichler, J. and Herrmann, R. G. - Analysis of the promoters of the single-copy genes for plastocyanin and subunit d of the chloroplast ATP synthase from spinach. Eur. J. Biochem. 190 (1990), 415-426.
3. Boulter, D., Haslett, B. G., Peacock, D., Ramshae, J. A. M., and Scawen, M. D. - Chemistry, function and evolution of plastocyanin. In: D. H. Northcote, ed., International Review of Biochemistry, Vol. 13: Plant Biochemistry II, University Park Press, Baltimore, pp. 1-40, 1977.
4. Boyer, H. W. and Roulland-Dossoix, D. - A complementation analysis of the restriction and modification of DNA in Escherichia coli. J. Mol. Biol. 41 (1969), 459-472.
5. Bradford, M. M. - A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976), 248-254.
6. Breyne, P., Gheysen, G., Jacobs, A., Van Montagu, M., and Depicker, A. - Effect of T-DNA configuration on gene expression. Mol. Gen. Genet. 235 (1992), 389-396.
7. Chory, J. - Light signals in leaf and chloroplast development. Photoreceptors and down-stream responses in search of a transduction pathway. New Biol. 3 (19911), 538-548.
8. Christou, P. and Yang, N.-S. - Developmental aspects of soybean (Glycine max) somatic embryogenesis. Annals of Botany 64 (1989), 225-234.
9. Deblaerre, R., Bytebier, B., De Greve, H., Deboeck, F., Schell, J., Van Montagu, M., and Leemans, J. - Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants. Nucl. Acids. Res. 13 (1985), 4777-4788.
10. Ditta. G., Stanfield, S., Corbin, D., and Helinski, D. R. - Broad host range DNA cloning system for Gram negative bacteria: Construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci. USA 77 (1980), 7347-7351.
11. Gao, J., Lee, J. M., and An, G. - The stability of foreign production in genetically modified plant cells. Plant Cell Rep. 10 (1991), 533-536.
12. Hamill, J. D., Rounsley, S., Spencer, A., Todd, G., and Rhodes, M. J. C. - The use of the polymerase chain reaction in plant transformation studies. Plant Cell Rep. 10 (1991), 221-224.
13. Hobbs, S. L. A., Kpodar, P., and De Long, C. M. O. - The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants. Plant Mol. Biol. 15 (1990), 851-864.
14. Modal, L., Bochardt, A., Nielsen, J. E., Mattson, O., and Okkels, F. T. - Detection, expression and specific elimination of endogenous β-glucuronidase activity in transgenic and non-transgenic plants. Plant Science 87 (1992), 115-122.
15. Hooykaas, P. J. J., Klapwjik, P., Nuti, M. P., Schilperoort, R. A., and Rorsch, A. - Transfer of the Agrobacterium tumefaciens Ti plasmid to avirulent Agrobacteria and to Rhizobium ex planta. J. Gen. Microbiol. 98 (1977), 477-484.
16. Jefferson, R. A. - Assaying chimeric genes in plants. The GUS gene fusion system. Plant Mol. Biol. Rep. 5 (1987), 387-405.
17. Jefferson, R. A., Kavanagh, T. A., and Bevan, M. W. - GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6 (1987), 3901-3907.
18. Junghans, H. and Metzlaff, M. - A simple and rapid method for the preparation of total plant DNA. Biol. Techniques 8 (1990), 176-182.
19. Koncz, C. and Schell, J. - The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimeric genes by a novel type of Agrobacterium tumefaciens. Mol. Gen. Genet. 204 (1986), 383-396.
20. Kosugi, S., Ohashi, Y., Nakajima, K., and Arai, Y. - An improved assay for β-glucuronidase in transformed cells: Methanol almost completely suppresses a putative endogenous β-glucuronidase activity. Plant Science 70 (1990), 133-140.
21. Kuhlemeier, C., Green, P. J., Chua, N. H. - Regulation of gene expression in higher plants. Ann. Rev. Plant Physiol. 38 (1987), 221-257.
22. Last, D. I. and Gray, J. C. - Synthesis and accumulation of pea plastocyanin in transgenic tobacco plants. Plant Mol. Biol. 14 (1990), 229-238.
23. Lehmann, U., Moldenhauer, D., Thomar, S., Diettrich, B., and Luckner, M. - Regeneration of plants from Digitalis lanata transformed with Agrobacterium tumefaciens carrying bacterial genes encoding neomycin phosphotransferase H and β-glucuronidase. J. Plant Physiol. 147 (1995), 53-57.
24. Luckner, M. and Diettrich, B. - The integration of cardenolide biosynthesis in the differentiation program "somatic embryogenesis" of Digitalis lanata. In: W. G. W. Kurz, ed., Primary and secondary metabolism of plant cell cultures II, pp. 117-124. Springer. Berlin, 1989.
25. Maniatis, T., Fritsch, E. F., and Sambrook, J. - Molecular Cloning. A Laboratory Manual. Cold Spring Habor Laboratory. New York, 1982.
26. Moldenhauer, D., Fürst, B., Diettrich, B., and Luckner, M. - Cardenolides in Digitalis lanata cells transformed with Ti-plasmids. Planta med. 56 (1990), 435-438.
27. Otten, L., Piotroviek, G., Hooykaas, P. J. J., and Dubois, M. - Identification of an Agrobacterium tumefaciens pTi B6 S3 virregion fragment that enhances the virulence of pTi C58. Mol. Gen. Genet. 199 (1985), 189-193.
28. Peach, C. and Veiten, J. - Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters. Plant Mol. Biol. 17 (1991), 49-60.
29. Pwee, H.-K. and Gray. J. C. - The pea plastocyanin promoter directs cell-specific but not full light-regulated expression in transgenic tobacco plants. Plant J. 3 (1993), 437-449.
30. Reinbothe, C., Diettrich, B., and Luckner, M. - Regeneration of plants from somatic embryos of Digitalis lanata. J. Plant Physiol. 137 (1990), 224-228.
31. Scheibner, J., Björk, L., Schulz, U., Diettrich, B., and Luckner, M. - Influence of light on cardenolide accumulation in somatic embryos of Digitalis lanata. J. Plant Physiol. 130 (1987), 211-219.
32. Scheibner, H., Diettrich, B., Schulz, U., and Luckner, M. - Somatic embryogenesis in Digitalis lanata cell cultures. Synchronization of development and cardenolide biosynthesis. Biochem. Physiol. Pflanzen 184 (1989), 311-320.
33. Scott, R. J. and Draper, J. - Transformation of carrot tissue derived from proembryogenic suspension cells: A useful model system for gene expression studies in plants. Plant Mol. Biol. 8 (1987), 265-274.
34. Tewes, A., Wappler, A., Peschke, M., Garve, R., and Nover, L. - Morphogenesis and embryogenesis in long-term cultures of Digitalis. Z. Pflanzenphysiol. 106 (1982), 311-324.
35. Töpfer, R., Schell, J., and Steinbiss, H. H. - Versatile cloning vectors for transient gene expression and direct gene transfer in plant cells. Nucl. Acids Res. 16 (1988), 8725.
36. Vorst, O., Kock, P., Lever, A., Weterings, B., Weisbeck, P., and Smeekens, S. - The promoter of the Arabidopsis thaliana plastocyanin gene contains a far upstream enhancer-like element involved in chloroplast-dependent expression. Plant J. 4 (1993), 933-945.
37. Vorst, O., van Dam, F., Oosterhoff-Teertstra, R., Smeekens, S., and Weisbeek, P. - Tissue-specific expression directed by an Arabidopsis thaliana pre-ferredoxin promoter in transgenic tobacco plants. Plant Mol. Biol. 14 (1990), 491-499.
38. Wang, H., Qi, M., and Cutler, A. J. - A simple method of preparing plant samples for PCR. Nucl. Acids Res. 21 (1993), 4153-4154.
39. Wurtele, E. S. and Bulka, K. - A simple, efficient method for the Agrobacrerium-mediated transformation of carrot callus cells. Plant Science 61 (1989), 253-262.
40. Zimmermann, J. L. - Somatic embryogenesis: A model for early development in higher plants. Plant Cell 5 (1993), 1411-1423.