Synthesis of Escherichia coli O9a polysaccharide requires the participation of two domains of WbdA, a mannosyltransferase encoded within the wb* gene cluster

Molecular Microbiology

Volumn 27, Issue 6, 1998, Pages 1213-1221

  Kido, Nobuo ^a   Sugiyama, Tsuyoshi ^b   Yokochi, Takashi ^b   Kobayashi, Hidemitsu ^c   Okawa, Yoshio ^c  

^a NAGOYA UNIVERSITY   (Japan)

^b AICHI MEDICAL UNIVERSITY   (Japan)

^c TOHOKU PHARMACEUTICAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL POLYSACCHARIDE; GENE PRODUCT; MANNOSE; MANNOSYLTRANSFERASE;

ARTICLE; BACTERIAL GENE; CARBOXY TERMINAL SEQUENCE; ESCHERICHIA COLI; GENE CLUSTER; GENE DELETION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; TRANSPOSON;

CONSERVED SEQUENCE; DNA, RECOMBINANT; ESCHERICHIA COLI; GENES, BACTERIAL; GENETIC COMPLEMENTATION TEST; LIPOPOLYSACCHARIDES; MANNOSE; MANNOSYLTRANSFERASES; MUTAGENESIS, INSERTIONAL; O ANTIGENS; PLASMIDS; POLYSACCHARIDES, BACTERIAL; PROTEIN STRUCTURE, SECONDARY; RESTRICTION MAPPING; SEQUENCE HOMOLOGY, NUCLEIC ACID;

EID: 0031932581     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1998.00765.x     Document Type: Article

References (28)

1. 13carbon-NMR spectra of complex carbohydrates. 1. α-D-mannopyranosyl residues in oligosaccharides and their implications for studies of glycoproteins and glycopeptides. J Am Chem Soc 106: 2400-2412.
2. Broom, J.E., Hill, D.F., Hughes, G., Jones, W.A., McNaughton, J.C., Stockwell, P.A., and Petersen, G.B. (1995) Sequence of a transposon identified as Tn 1000 (gamma delta). DNA Seq 5: 185-189.
3. Cheah, K-C, and Manning, P.A. (1993) Inactivation of the Escherichia coli B41 (O101:K99/F41) rfb gene encoding an 80-kDa polypeptide results in the synthesis of an antigenically altered lipopolysaccharide in E. coli K-12. Gene 123: 9-15.
4. Chou, P.Y., and Fasman, G.D. (1974) Prediction of protein conformation. Biochemistry 13: 222-244.
5. 13carbon-nuclear magnetic resonance. J Am Chem Soc 96: 8081-8087.
6. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., and Smith, F. (1956) A colorimetric method for determination of sugars and related substances. Anal Chem 28: 350-356.
7. Geremia, R.A., Petroni, E.A., Ielpi, L., and Henrissat, B. (1996) Towards a classification of glycosyltransferases based on amino acid sequence similarities: prokaryotic-mannosyltransferases. Biochem J 318: 133-138.
8. Guyer, M.S. (1983) Uses of the transposon γδ in the analysis of cloned genes. Methods Enzymol 101: 362-369.
9. Haraguchi, G.E., Zähringer, U., Jann, B., Jann, K., Hull, R.A., and Hull, S.I. (1991) Genetic characterization of the O4 polysaccharide gene cluster from Escherichia coli. Microbial Pathogenesis 10: 351-361.
10. Hitchcock, P.J., and Brown, T.M. (1983) Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver stained polyacrylamide gel electrophoresis. J Bacteriol 154: 269-277.
11. Kido, N., Ohta, M., Iida, K., Hasegawa, T., Ito, H., Arakawa, Y. et al. (1989) Partial deletion of the cloned rfb gene of Escherichia coli O9 results in synthesis of a new O-antigenic lipopolysaccharide. J Bacteriol 171: 3629-3633.
12. Kido, N., Torgov, V.I., Sugiyama, T., Uchiya, K., Sugihara, H., Komatsu, T. et al. (1995) Expression of the O9 polysaccharide of Escherichia coli: sequencing of the E. coli O9 rfb gene cluster, characterization of mannosyl transferases, and evidence for an ATP-binding cassette transport system. J Bacteriol 177: 2178-2187.
13. Kido, N., Morooka, N., Paeng, N., Ohtani, T., Kobayashi, H., Shibata, N. et al. (1997) Production of monoclonal antibody discriminating serological difference in Escherichia coli O9 and O9a polysaccharides. Microbiol Immunol 41: 519-525.
14. 13C NMR analyses of acid-labile oligomannosyl residues. Arch Biochem Biophys 278: 195-204.
15. Kobayashi, H., Kojimahara, T., Takahashi, K., Takikawa, M., Takahashi, S., Shibata, N. et al. (1991a) Structural determination of D-mannans of pathogenic yeast Candida sellatoidea type I strains TIMM and ATCC 11006 compared to IFO 1397. Carbohydr Res 214: 131-145.
16. Kobayashi, H., Giummelly, P., Takahashi, S., Ishida, M., Sato, J., Takaku, M. et al. (1991b) Candida albicans serotype A strains grown in yeast extract-added Sabouraud liquid medium at pH2.0, elaborating mannans without α-1, 2 linkage and phosphate group. Biochem Biophys Res Commun 175: 1003-1009.
17. Kobayashi, H., Matsuda, K., Ikeda, T., Suzuki, M., Takahashi, S., Suzuki, A. et al. (1994) Structures of cell wall mannans of pathogenic Candida tropicalis IFO 0199 and IFO 1647 yeast strains. Infect Immun 62: 615-622.
18. Kogan, G., Haraguchi, G., Hull, S.I., Hull, R.A., Shashkov, A.S., Jann, B., and Jann, K. (1993) Structural analysis of O4-related polysaccharides from recombinant Escherichia coli: changes in the O-specific polysaccharide induced by cloning of the rfb genes. Eur J Biochem 214: 259-265.
19. Morona, R., Macpherson, D.F., Bosch, L.V.D., Carlin, N.I.A., and Manning, P.A. (1995) Lipopolysaccharide with an altered O-antigen produced in Escherichia coli K-12 harbouring mutated, cloned Shigella flexneri rfb genes. Mol Microbiol 18: 209-223.
20. Reeves, P.R., Hobbs, M., Valvano, M.A., Skurnik, M., Whitfield, C., Coplin, D., et al. (1996) Bacterial polysaccharide synthesis and gene nomenclature. Trends Microbiol 4: 495-503.
21. Sancar, A., Wharton, R.P., Seltzer, S., Kacinski, B.M., Clarke, N.D., and Rupp, W.D. (1981) Identification of the uvrA gene product. J Mol Biol 148: 45-62.
22. Smith, G.R. (1991) Conjugational recombination in E. coli: myths and mechanisms. Cell 64: 19-27.
23. Stevenson, G., Neal, B., Liu, D., Hobbs, M., Packer, N.H., Batley, M. et al. (1994) Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster. J Bacteriol 176: 4144-4156.
24. Sugiyama, T., Kido, N., Komatsu, T., Ohta, M., Jann, K., and Kato, N. (1991) Expression of the cloned Escherichia coli O9 rfb gene in various mutant strains of Salmonella typhimurium. J Bacteriol 173: 55-58.
25. Sugiyama, T., Kido, N., Komatsu, T., Ohta, M., Jann, K., Jann, B. et al. (1994) Genetic analysis of Escherichia coli O9 rfb: identification and DNA sequence of phosphomannomutase and GDP-mannose pyrophosphorylase genes. Microbiology 140: 59-71.
26. Tsai, C.M., and Frasch, C.E. (1982) A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 119: 115-119.
27. Weisgerber, C., Jann, B., and Jann, K. (1984) Biosynthesis of the O9 antigen of Escherichia coli: core structure of rfe mutant as indication of assembly mechanism. Eur J Biochem 140: 553-556.
28. Xiang, S-H, Hobbs, M., and Reeves, P.R. (1994) Molecular analysis of the rfb gene cluster of a group D2 Salmonella enterica strain: evidence for its origin from an insertion sequence-mediated recombination event between group E and D1 strains. J Bacteriol 176: 4357-4365.