Protein profiles and antifungal activities of kernel extracts from corn genotypes resistant and susceptible to Aspergillus flavus

Journal of Food Protection

Volumn 61, Issue 1, 1998, Pages 98-102

  Guo, B Z ^a   Brown, R L ^b   Lax, A R ^b   Cleveland, T E ^b   Russin, J S ^c   Widstrom, N W ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b SOUTHERN REGIONAL RESEARCH CENTER   (United States)

^c LOUISIANA STATE UNIVERSITY AGRICULTURAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPERGILLUS FLAVUS; EMBRYOPHYTA; FUNGI; ZEA MAYS;

EID: 0031930733     PISSN: 0362028X     EISSN: None     Source Type: Journal    
DOI: 10.4315/0362-028X-61.1.98     Document Type: Article

References (31)

1. Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
2. Brown, R. L., P. J. Cotty, T. E. Cleveland, and N. W. Widstrom. 1993. Living maize embryo influences accumulation of aflatoxin in maize kernels. J. Food Prot. 56:967-971.
3. Cleveland, T. E., and D. Bhatnagar. 1992. Molecular strategies for reducing aflatoxin levels in crops before harvest, p. 205-228. In D. Bhatnagar and T. E. Cleveland (ed.), Molecular approaches to improving food quality and safety. Van Nostrand Reinhold, New York.
4. Cordero, M. J., D. Raventós, and B. S. Segundo. 1992. Induction of PR protein in germinating maize seeds infected with the fungus Fusarium moniliforme. Physiol. Mol. Plant Pathol. 41:189-200.
5. Darnetty, J. F. Leslie, S. Muthukrishnan, M. Swegle, A. J. Vigers, and C. P. Selitrennikoff. 1993. Variability in antifungal proteins in the grains of maize, sorghum and wheat. Physiol. Plant. 88:339-349.
6. Duvick, J. P., T. Rood, A. Gururaj Rao, and D. R. Marshak. 1992. Purification and characterization of a novel antimicrobial peptide from maize (Zea mays L.) kernels. J. Biol. Chem. 267:18814-18820.
7. Garcia-Olmedo, F., G. Salcedo, R. Sanchez-Monge, L. Gomez, J. Royo, and P. Carbonero. 1987. Plant proteinaceous inhibitors of proteinases and alpha-amylases. Plant Mol. Cell Biol. 4:275-334.
8. Guo, B. Z. 1995. Mechanisms of resistance to aflatoxin accumulation by Aspergillus flavus in maize genotype GT-MAS:gk. Ph.D. Dissertation, Louisiana State University, Baton Rouge.
9. Guo, B. Z., J. S. Russin, T. E. Cleveland, R. L. Brown, and K. E. Damann. 1996. Evidence for cutinase production by Aspergillus flavus and its possible role in infection of corn kernels. Phytopathology 86:824-829.
10. Guo, B. Z., J. S. Russin, T. E. Cleveland, R. L. Brown, and N. W. Widstrom. 1995. Wax and cutin layers in maize kernels associated with resistance to aflatoxin production by Aspergillus flavus. J. Food Prot. 58:296-300.
11. Huang, Z., D. G. White, and G. A. Payn. 1997. Corn seed proteins inhibitory to Aspergillus flavus and aflatoxin biosynthesis. Phytopathology 87:622-627.
12. Huynh, Q. K., C. M. Hironaka, E. B. Levine, C. E. Smith, J. R. Borgmeyer, and D. M. Shah. 1992. Antifungal proteins from plants: purification, molecular cloning, and antifungal properties of chitinases from maize seed. J. Biol. Chem. 267:6635-6640.
13. Jach, G., B. Görnhardt, J. Mundy, J. Logemann, E. Pinsdorf, R. Leah, J. Schell, and C. Maas. 1995. Enhanced quantitative resistance against fungal disease by combinatorial expression of different barley antifungal proteins in transgenic tobacco. Plant J. 8:97-109.
14. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 277:680-685.
15. Leah, R., H. Tommerup, I. Svendsen, and J. Mundy. 1991. Biochemical and molecular characterization of three barley seed proteins with antifungal properties. J. Biol. Chem. 266:1564-1573.
16. Lillehoj, E. B., and J. H. Wall. 1987. Decontamination of aflatoxin-contaminated maize grain, p. 260-279. In M. S. Zuber, E. B. Lillehoj, and B. L. Renfro (ed.), Aflatoxin in maize: a proceedings of the workshop. CIMMYT, Mexico, D.F.
17. Logemann, J., G. Jach, H. Tommerup, J. Mundy, and J. Schell. 1992. Expression of a barley ribosome-inactivating protein leads to increased fungal protection in transgenic tobacco plants. Bio/ Technology 10:305-308.
18. McMillian, W. W., N. W. Widstrom, and D. M. Wilson. 1993. Registration of GT-MAS:gk maize germplasm. Crop Sci. 33:882.
19. Nagarajan, V., and R. V. Bhat. 1972. Factors responsible for varietal differences in aflatoxin production in maize. J. Agric. Food Chem. 20:911-914.
20. Neucere, J. N., and H. J. Zeringue, Jr. 1987. Inhibition of Aspergillus flavus growth by fractions of salt-extracted proteins from maize kernel. J. Agric. Food Chem. 35:806-808.
21. Newberg, D. S., and J. M. Concon. 1985. Lectins in rice and corn endosperm. J. Agric. Food Chem. 33:685-687.
22. O'Farrell, P. H. 1975. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250:4007-4021.
23. Raikhel, N. V., and T. A. Wilkins. 1987. Isolation and characterization of a complementary DNA clone encoding wheat germ agglutinin. Proc. Natl. Acad. Sci. USA 84:6745-6749.
24. Roberts, W. K., B. E. Laue, and C. P. Selitrennikoff. 1988. Antifungal proteins from plants. Ann. N.Y. Acad. Sci. 544:141-151.
25. Roberts, W. K., and C. P. Selitrennikoff. 1990. Zeamatin, an antifungal protein from maize with membrane-permeabilizing activity. J. Gen. Microbiol. 136:1771-1778.
26. Schägger, H., and G. von Jagow. 1987. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:368-379.
27. Scott, G. E., and N. Zummo. 1990. Preharvest kernel infection by Aspergillus flavus for resistant and susceptible maize hybrids. Crop Sci. 30:381-383.
28. Scott, G. E., and N. Zummo. 1992. Registration of Mp420 germplasm line of maize. Crop Sci. 32:1296.
29. Walsh, T. A., A. E. Morgan, and T. D. Hey. 1991. Characterization and molecular cloning of a proenzyme form of a ribosome-inactivating proteins from maize. J. Biol. Chem. 266:23422-23427.
30. Widstrom, N. W., W. W. McMillian, and D. M. Wilson. 1987. Segregation for resistance to aflatoxin contamination among seeds on an ear of hybrid maize. Crop Sci. 27:961-963.
31. Wilson, C. M. 1987. Proteins of kernel, p. 273-310. In Watson, S. A., and P. E. Raustad (ed.), Corn: chemistry and technology. American Association of Cereal Chemistry, St. Paul, MN.