Milk-Tween agar, a semiselective medium for isolation and differentiation of Pseudomonas syringae pv. syringae, Pseudomonas syringae pv. phaseolicola and Xanthomonas axonopodis pv. phaseoli

Journal of Microbiological Methods

Volumn 32, Issue 1, 1998, Pages 65-72

  Goszczynska, T ^a   Serfontein, J J ^a  

^a PLANT PROTECTION RESEARCH INSTITUTE   (South Africa)

Author keywords

Detection; Differentiation; Milk Tween medium

Indexed keywords

AGAR; CEFALEXIN; CYCLOHEXIMIDE; PEPTONE; POLYSORBATE 80; VANCOMYCIN;

ARTICLE; BACTERIAL GROWTH; BACTERIUM DETECTION; BACTERIUM ISOLATION; BEAN; CULTURE MEDIUM; MILK; NONHUMAN; PLANT; PRIORITY JOURNAL; PSEUDOMONAS SYRINGAE; XANTHOMONAS;

EID: 0031930599     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(98)00005-0     Document Type: Article

References (27)

1. Aggour A.R., Coyne D.P., Vidaver A.K., Eskridge K.M. Transmission of the common blight pathogen in bean seed. J. Am. Soc. Hort. Sci. 114(6):1989;1002-1008.
2. Angeles-Ramos R., Vidaver A.K., Flynn P. Characterization of epiphytic Xanthomonas campestris pv. phaseoli and pectolytic Xanthomonads recovered from symptomless weeds in the Dominican Republic. Phytopathology. 81:1991;677-681.
3. Audy P., Braat C.E., Saindon G., Huong H.C., Laroche A. A rapid and sensitive PCR-based assay for concurrent detection of bacteria causing common and halo blights in bean seed. Phytopathology. 86:1996;361-366.
4. Basu P.K. Glucose inhibition of the characteristic melanoid pigment of Xanthomonas phaseoli var. fuscans. Can. J. Bot. 52:1974;2203-2206.
5. Basu P.K., Wallen V.R. Factors affecting virulence and pigment production of Xanthomonas phaseoli var. fuscans. Can. J. Bot. 45:1967;2367-2374.
6. J.F. Bradbury, Guide to plant pathogenic bacteria, C.A.B International Mycological Institute, Ferry Lane, Kew, Surrey, England, 1986.
7. Claffin L.E., Vidaver A.K., Sasser M. MXP, a semiselective medium for Xanthomonas campestris pv. phaseoli. Phytopathology. 77:1987;730-734.
8. Copeland L.O., Adams M.W., Bell D.C. An improved seed programme for maintaining disease-free seed of field beans (Phaseolus vulgaris). Seed Sci. Technol. 3:1975;719-724.
9. Dhanvantari B.N., Brown R.J. YSSM-XP medium for Xanthomonas campestris pv. phaseoli. Can. J. Plant Pathol. 15:1993;168-174.
10. A.C. Hayward, Preliminary diagnosis of plant diseases caused by bacteria, in: P.C. Fahy, G.J. Persley (Eds.), Plant bacterial diseases. A diagnostic guide, Academic Press, Australia, 1983, pp. 5-7.
11. Gillbertson R.L., Rand R.E., Hagedorn D.J. Survival of Xanthomonas campestris pv. phaseoli and pectolytic strains of X. campestris in bean debris. Plant Dis. 74:1990;322-327.
12. Hoitink H.A.J., Hagedorn D.J., McCoy E. Survival, transmission and taxonomy of Pseudomonas syringae van Hall, the causal organism of bacterial brown spot of bean (Phaseolus vulgaris L.). Can. J. Microbiol. 14:1968;437-441.
13. Jackson L.E., Moser P.E. Development of medium for isolation and differentiation of three bacterial plant pathogens. Annu. Rep. Bean Improvment Coop. 37:1994;23-24.
14. King E.O., Ward M.K., Raney D.E. Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44:1954;301-307.
15. McGuire R., Jones J.B. Tween media for semiselective isolaton of Xanthomonas campestris pv. vesicatoria from soil and plant material. Plant Dis. 70:1986;887-891.
16. Mohan S.K., Schaad N.W. An improved agar plating assay for detecting Pseudomonas syringae pv. syringae and Pseudomonas syringae pv. phaseolicola in contaminated bean seed. Phytopathology. 77:1987;1390-1395.
17. Moser P.E., Cowan L., Massoth H. A semiselective medium plating assay for detection and differentiation of bacterial blight pathogens of bean and pea. Annu. Rep. Bean Improvment Coop. 37:1994;25-26.
18. Niepold F. Development of a method to obtain monospecific antibodies directed against a 31 kD protein of Pseudomonas syringae pv. syringae. J. Phytopathol. 136:1992;137-146.
19. Ralph W. Problems in testing and control of seed-borne bacterial pathogens: a critical evaluation. Seed Sci. Technol. 5:1987;735-752.
20. Schaad N.W. Detection of seedborne bacterial plant pathogens. Plant Dis. 66:1982;885-890.
21. Schaad N.W., Cheong S., Tamaki S., Hatziloukas E., Panopoulos N.J. A combined biological and enzymatic amplification (BIO-PCR) technique to detect Pseudomonas syringae pv. phaseolicola in bean seed extract. Phytopathology. 85:1995;234-248.
22. Serfontein J.J., Hattingh M.J. Characterization of Pseudomonas syringae pv. tomato by numerical analysis of phenotypic features and total soluble proteins. J. Phytopathol. 128:1990;220-234.
23. Sleesman J.P., Leben C. Preserving phytopathogenic bacteria at -70°C or with silica gel. Plant Dis. Rep. 62:1978;910-913.
24. Taylor J.D. Bacteriophage and serological methods for the identification of Pseudomonas phaseolicola (Burgh.) Dowson. Ann. Appl. Biol. 93:1970;387-395.
25. Taylor J.D., Dudley C.L., Presley L. Studies of halo-blight seed infection and disease transmission in dwarf beans. Ann. Appl. Biol. 93:1979;167-172.
26. Van Vuurde J.W.L., Van den Bovenkamp L., Birnbaum G.W. Immunofluorescence microscopy and enzyme-linked immunosorbent assay as potential routine tests for the detection of Pseudomonas syringae pv. phaseolicola and Xanthomonas campestris pv. phaseoli in bean seed. Seed Sci. Technol. 11:1983;547-559.
27. Wyatt G.M., Turner J.G., Morgan M.R.A. Rapid and specific detection of Pseudomonas syringae pv. phaseolicola by immunological methods. Food Agric. Immunol. 1:1989;53-63.