Two separate conserved domains of eukaryotic DNA topoisomerase I bind to each other and reconstitute enzymatic activity

Chromosoma

Volumn 107, Issue 3, 1998, Pages 211-215

  Park, Hangil ^a   Sternglanz, Rolf ^a  

^a STONY BROOK UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA TOPOISOMERASE; POLYPEPTIDE;

ARTICLE; ASSAY; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; GENETIC CONSERVATION; MUTANT; NONHUMAN; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; BINDING SITES; CONSERVED SEQUENCE; DNA TOPOISOMERASES, TYPE I; EUKARYOTIC CELLS; GENETIC COMPLEMENTATION TEST; HYBRID CELLS; PEPTIDE FRAGMENTS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 0031926693     PISSN: 00095915     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004120050299     Document Type: Article

References (23)

1. Bartel PL, Roecklein JA, SenGupta D, Fields S (1996) A protein linkage map of Escherichia coli bacteriophage T7. Nat Genet 12:72-77
2. Benedetti P, Fiorani P, Capuani L, Wang JC (1993) Camptothecin resistance from a single mutation changing glycine 363 of human DNA topoisomerase I to cysteine. Cancer Res 53:4343-4348
3. Brill S, DiNardo S, Voelkel-Meiman K, Sternglanz R (1987) Need for DNA topoisomerase activity as a swivel for DNA replication and for transcription of ribosomal RNA. Nature 312:414-416
4. Champoux JJ (1990) Mechanistic aspects of type-I topoisomerases. In: Cozzarelli NR, Wang JC (eds) DNA topology and its biological effects. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp 217-242
5. Chien C-T, Bartel PL, Sternglanz R, Fields S (1991) The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc Natl Acad Sci USA 88:9578-9582
6. Eng W, Pandit SD, Sternglanz R (1989) Mapping of the active site tyrosine of eukaryotic DNA topoisomerase I. J Biol Chem 264:13373-13376
7. Fields S, Song O-K (1989) A novel genetic system to detect protein-protein interactions. Nature 340:245-247
8. Fields S, Sternglanz R (1994) The two-hybrid system: an assay for protein-protein interactions. Trends Genet 10:286-292
9. Goto T, Laipis T, Wang JC (1984) The purification and characterization of DNA topoisomerases I and II of the yeast Saccharomyces cerevisiae. J Biol Chem 259:10422-10429
10. Hollenberg SM, Sternglanz R, Cheng PF, Weintraub H (1995) Identification of a new family of tissue-specific basic helix-loop-helix proteins with a two-hybrid system. Mol Cell Biol 15:3813-3822
11. James P, Halladay J, Craig EA (1996) Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast. Genetics 144:1425-1436
12. Knab AM, Fertala J, Bjornsti M-A (1993) Mechanisms of camptothecin resistance in yeast DNA topoisomerase I mutants. J Biol Chem 268:22322-22330
13. Kretzchmar M, Meisterernst M, Roeder RG (1993) Identification of human DNA topoisomerase I as a cofactor for activator-dependent transcription by RNA polymerase II. Proc Natl Acad Sci USA 90:11508-11512
14. Levin NA, Bjornsti M-A, Fink GR (1993) A novel mutation in DNA topoisomerase I of yeast causes DNA damage and RAD9-dependent cell cycle arrest. Genetics 133:799-814
15. Lue N, Sharma A, Mondragon A, Wang JC (1995) A 26 kDa yeast DNA topoisomerase I fragment: crystallographic structure and mechanistic implications. Structure 3:1315-1322
16. Merino A, Madden KR, Lane WS, Champoux JJ, Reinberg D (1993) DNA topoisomerase I is involved in both repression and activation of transcription. Nature 365:227-232
17. Rose MD, Winston F, Hieter P (1988) Methods in yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
18. Stewart L, Ireton GC, Champoux JJ (1996) The domain organization of human topoisomerase I. J Biol Chem 271:7602-7608
19. Stewart L, Ireton GC, Champoux JJ (1997) Reconstitution of human topoisomerase I by fragment complementation. J Mol Biol 269:355-372
20. Thrash C, Voelkel K, DiNardo S, Sternglanz R (1984) Identification of S. cerevisiae DNA topoisomerase I mutants. J Biol Chem 259:1375-1377
21. Thrash C, Bankier AT, Barrell BG, Sternglanz R (1985) Cloning, characterization and sequence of the yeast DNA topoisomerase I gene. Proc Natl Acad Sci USA 82:4374-4378
22. Uemura H, Pandit S, Jigami Y, Sternglanz R (1996) Mutations in GCR3, a gene involved in the expression of glycolytic genes in Saccharomyces cerevisiae, suppress the temperature-sensitive growth of hpr1 mutants. Genetics 142:1095-1103
23. Wang JC (1996) DNA topoisomerases. Annu Rev Biochem 65:635-692