New procedures for rapid screening of leaf lipid components from Arabidopsis

Phytochemical Analysis

Volumn 9, Issue 2, 1998, Pages 53-57

  Christie, William W ^a  

^a SCOTTISH CROP RESEARCH INSTITUTE   (United Kingdom)

Author keywords

Arabidopsis thaliana; High performance liquid chromatography; Lipids; Solid phase extraction

Indexed keywords

CHLORINE COMPOUNDS; EXTRACTION; ION EXCHANGE; LIGHT SCATTERING; PHASE SEPARATION; PHOSPHOLIPIDS; SCREENING;

ARABIDOPSIS; ARABIDOPSIS THALIANA; GLYCO LIPIDS; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; PLANT LIPIDS; RAPID EXTRACTION; RAPID SCREENING; ROUTINE ANALYSIS; SIMPLE++; SOLID-PHASE EXTRACTION;

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

2 PROPANOL; AMPHOLYTE; CHLOROFORM; GLYCOLIPID; LIPID; PHOSPHOLIPID; PROPYLAMINE; SEPHADEX;

ANALYTIC METHOD; ARABIDOPSIS; ARTICLE; CONTROLLED STUDY; ESTERIFICATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIPID ANALYSIS; NONHUMAN; PHYTOCHEMISTRY; PLANT LEAF; SOLID PHASE EXTRACTION;

ARABIDOPSIS;

EID: 0031925804     PISSN: 09580344     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1099-1565(199803/04)9:2<53::AID-PCA391>3.0.CO;2-U     Document Type: Article

References (17)

1. Arondel, V., Lemieux, B., Hwang, I., Gibson, S., Goodman, H. M. and Somerville, C. R. (1992). Map-based cloning of a gene controlling ω-3 fatty acid desaturation in Arabidopsis. Science, 258, 1353-1354.
2. Browse, J. and Somerville, C. (1994). Glycerolipids. In Arabidopsis (Meyerowitz, E. M. and Somerville, C. R. eds), pp. 881-936, Cold Spring Harbor Press, New York.
3. Christie, W. W. (1982). A simple procedure for rapid transmethylation of glycerolipids and cholesteryl esters. J. Lipid Res. 23, 1072-1075.
4. Christie, W. W. (1992). Solid-phase extraction columns in the analysis of lipids. In Advances in Lipid Methodology-One (Christie, W. W., ed.), pp. 1-17, Oily Press, Dundee.
5. Christie, W. W. (1993). Preparation of lipid extracts from tissues. In Advances in Lipid Methodology-Two (Christie, W. W., ed.), pp. 195-213, Oily Press, Dundee.
6. Christie, W. W. (1996). Separation of phospholipid classes by high-performance liquid chromatography. In Advances in Lipid Methodology-Three (Christie, W. W., ed.), pp. 77-107, Oily Press, Dundee.
7. Christie, W. W. and Urwin, R. A. (1995). Separation of lipid classes from plant tissues by HPLC on chemically bonded stationary phases. J. High Resolut. Chromatogr. 18, 97-100.
8. Dormann, P., Hoffman-Benning, S., Balbo, I. and Benning, C. (1995). Isolation and characterization of an Arabidopsis mutant deficient in the thylakoid lipid digalactosyldiacylglycerol. Plant Cell, 7, 1801-1810.
9. Ebeler, S. E. and Shibamoto, T. (1993). Overview and recent developments in solid-phase extraction for separation of lipid classes. In Lipid Chromatographic Analysis (Shibamoto. T., ed.), pp. 1-48, Dekker, New York.
10. Egberts, J. and Buiskool, R. (1988). Isolation of the acidic phospholipid phosphatidylglycerol from pulmonary surfactant by sorbent extraction chromatography. Clin. Biochem. 34, 163-164.
11. Folch, J., Lees, M. and Stanley, G. H. S. (1957). A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497-509.
12. Heinz, E. (1996). Plant glycolipids: structure, isolation and analysis. In Advances in Lipid Methodology-Three (Christie, W. W., ed.), pp. 211-332, Oily Press, Dundee.
13. Kaluzny, M. A., Duncan, L. A., Merritt, M. V. and Epps, D. E. (1985). Rapid separation of lipid classes in high yield and purity using bonded phase columns. J. Lipid Res. 26, 135-140.
14. Kates, M. and Eberhardt, F. M. (1957). Isolation and fractionation of leaf phosphatides. Can. J. Bot. 35, 895-905.
15. Nichols, B. W. (1963). Separation of the lipids of photosynthetic tissues: improvements in analysis by TLC. Biochim. Biophys. Acta 70, 417-422.
16. Okuley, J., Lightner, J., Feldmann, K., Yadav, N., Lark, E. and Browse, J. (1994). Arabidopis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis. Plant Cell. 6, 147-158.
17. Wuthier, R. E. (1996). Purification of lipids from nonlipid contaminants on Sephadex bead columns. J. Lipid Res. 7, 558-561.