Prominent occurrence of ribosomes from an uncultured bacterium of the Verrucomicrobiales cluster in grassland soils

Letters in Applied Microbiology

Volumn 26, Issue 3, 1998, Pages 219-223

  Felske, A ^a   Akkermans, A D L ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTROPHORESIS; RNA; SOILS;

16S RDNA SEQUENCE; 16S RRNA; AMPLICON SEQUENCES; GRASSLAND SOILS; METABOLIC ACTIVITY; MICROBIAL COMMUNITIES; SEQUENCE IDENTITY; SOUTHERN BLOT HYBRIDIZATION; SPECIFIC PRIMERS; TEMPERATURE GRADIENT GEL ELECTROPHORESIS;

BACTERIA;

RNA 16S;

ACTIVATED SLUDGE; ARTICLE; CONTROLLED STUDY; GEL ELECTROPHORESIS; IMAGE ANALYSIS; NETHERLANDS; NONHUMAN; PHYLOGENY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RIBOSOME; SILVER STAINING; SOIL MICROFLORA; SOUTHERN BLOTTING; VERRUCOMICROBIALES;

BACTERIA (MICROORGANISMS); VERRUCOMICROBIALES;

EID: 0031921271     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1472-765X.1998.00322.x     Document Type: Article

References (20)

1. Albrecht, W., Fischer, A., Smida, J. and Stackebrandt, E. (1987) Verrucomicrobium spinosum, a cubacterium representing an ancient line of descent. Systematic and Applied Microbiology 10, 57-62.
2. Bond, P.L., Hugenholtz, P., Keller, J. and Blackall, L. (1995) Bacterial community structures of phosphate-removing and non-phosphate-removing activated sludges from sequencing hatch reactors. Applied and Environmental Microbiology 61, 1910-1916.
3. Borneman, J. and Triplett, E.W. (1997) Molecular microbial diversity in soils from Eastern Amazonia: evidence for unusual microorganisms and microbial population shifts associated with deforestation. Applied and Environmental Microbiology 63, 2647-2653.
4. Felske, A. and Akkermans, A.D.L. (1997) Spatial homogeneity of the most abundant bacterial 16S rRNA molecules in grassland soils. Microbiol. Ecology in press.
5. Felske, A., Engelen, B., Nübel, U. and Backhaus, H. (1996) Direct ribosome isolation from soil to extract bacterial rRNA for community analysis. Applied and Environmental Microbiology 62, 4162-4167.
6. Felske, A., Rheims, H., Wolterink, A., Stackebrandt, E. and Akkermans, A.D.L. (1997) Ribosome analysis reveals prominent activity of an uncultured member of the class Actinobacteria in grassland soils. Microbiology 143, 2983-2989.
7. Hedlund, B.P., Gosink, J.J. and Staley, J.T. (1996) Phylogeny of Prosthecobacter, the fusiform Caulobacters: members of a recently discovered division of Bacteria. International Journal of Systematic Bacteriology 46, 960-966.
8. Heuer, H. and Smalla, K. (1997) Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) for studying soil microbial communities, In Modern Soil Microbiology ed. van Elsas, J.D., Wellington, E.M.H. and Trevors, J.T. pp. 353-373. New York: Marcel Dekker Inc.
9. Hiorns, W.D., Methé, B.A., Nierzwicki-Bauer, S.A. and Zehr, J.P. (1997) Bacterial diversity in Adirondack mountain lakes as revealed by 16S rRNA sequences. Applied and Environmental Microbiology 63, 2957-2960.
10. Lee, S.-Y., Bollinger, J., Bezdicek, D. and Ogram, A. (1996) Estimation of the abundance of an uncultured soil bacterial strain by a competitive quantitative PCR method. Applied and Environmental Microbiology 62, 3787-3793.
11. Liesack, W. and Stackebrandt, E. (1992) Occurrence of novel groups of the domain Bacteria as revealed by analysis of genetic material isolated from an Australian terrestrial environment. Journal of Bacteriology 174, 5072-5078.
12. Muyzer, G., De Waal, E.G. and Uitterlinden, A.G. (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied and Environmental Microbiology 59, 695-700.
13. Rosenbaum, V. and Riesner, D. (1987) Temperature-gradient gel electrophoresis - thermodynamic analysis of nucleic acids and proteins in purified form and in cellular extracts. Biophysical Chemistry 26, 235-246.
14. Schlesner, H. (1987) Verrucomicrobium spinosum gen. nov., sp. nov.: a fimbriated prosthecate bacterium. Systematic and Applied Microbiology 10, 54-56.
15. Stienstra, A.W., Klein Gunnewiek, P. and Laanbroek, H.J. (1994) Repression of nitrification in soils under climax grassland vegetation. FEMS Microbiology Ecology 14, 45-52.
16. Strunk, O. and Ludwig, W. (1995) ARB - a software environment for sequence data. Department of Microbiology, Technical University of Munich, München, Germany.
17. Torsvik, V., Goksøyr, J. and Daae, F.L. (1990) High diversity in DNA of soil bacteria. Applied and Environmental Microbiology 56, 782-787.
18. Ueda, T., Suga, Y. and Matsuguchi, T. (1995) Molecular phylogenetic analysis of a soil microbial community in a soybean field. European Journal of Soil Science 46, 415-421.
19. Wagner, R. (1994) The regulation of ribosomal RNA synthesis and bacterial cell growth. Archives of Microbiology 161, 100-106.
20. Ward-Rainey, N., Rainey, F.A., Schlesner, H. and Stackebrandt, E. (1995) Assignment of hitherto unidentified 16S rDNA species to a main line of descent within the domain Bacteria. Microbiology 141, 3247-3250.