Low-voltage separation of phosphoamino acids by silica gel thin-layer electrophoresis in a DNA electrophoresis cell

BioTechniques

Volumn 24, Issue 3, 1998, Pages 344-346

  Hardin, Shane C ^a   Wolniak, Stephen M ^a  

^a UNIVERSITY OF MARYLAND   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; MYELIN BASIC PROTEIN; PHOSPHOAMINO ACID; PROTEIN KINASE; SERINE; SILICA GEL; THREONINE; TYROSINE;

AMINO ACID ANALYSIS; ARTICLE; GEL ELECTROPHORESIS; ISOTOPE LABELING; MAIZE; MEMBRANE; NONHUMAN; PROTEIN DEGRADATION; PROTEIN PHOSPHORYLATION; TECHNIQUE;

ZEA MAYS;

EID: 0031919751     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98243bm01     Document Type: Article

References (6)

1. Boyle, W.J., P. Geer and T. Hunter. 1991. Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates, p. 110-149. In T. Hunter and B.M. Sefton (Eds.), Methods in Enzymology, Vol. 201. Academic Press, New York.
2. Cooper, J.A., B.M. Sefton and T. Hunter. 1983. Detection and quantification of phosphotyrosine in proteins, p. 387-402. In J.D. Corbin and J.G. Hardman (Eds.), Methods in Enzymology, Vol. 99. Academic Press, New York.
3. Duclos, B., S. Marcandier and A.J. Cozzone. 1991. Chemical properties and separation of phosphoamino acids by thin-layer chromatography and/or electrophoresis, p. 10-21. In T. Hunter and B.M. Sefton (Eds.), Methods in Enzymology, Vol. 201. Academic Press, New York.
4. Kamps, M.P. and B.M. Sefton. 1989. Acid and base hydrolysis of phosphoproteins bound to Immobilon facilitates analysis of phosphoamino acids in gel-fractionated proteins. Anal. Biochem. 176:22-21.
5. Neufeld, E., H.J. Goren and D. Boland. 1989. Thin-layer chromatography can resolve phosphotyrosine, phosphoserine and phosphothreonine in a protein hydrolysate. Anal. Biochem. 777:138-143.
6. Pataki, G. 1969. Techniques of thin-layer chromatography in amino acid and peptide chemistry. Ann Arbor-Humphrey Science Publishers, Ann Arbor.