High-throughput screening for known mutations by automated analysis of single sequencing reactions

BioTechniques

Volumn 24, Issue 5, 1998, Pages 832-835

  Kristensen, Tom ^b   Kristensen, Vessela Nedelcheva ^a   Børresen Dale, Anne Lise ^a  

^a INSTITUTE OF CANCER RESEARCH   (Norway)

^b UNIVERSITY OF OSLO   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

AROMATASE; DNA POLYMERASE;

ARTICLE; AUTOANALYSIS; CONTROLLED STUDY; DNA SEQUENCE; GENE MUTATION; GENOTYPE; HUMAN; HUMAN CELL; LEUKOCYTE; POLYMERASE CHAIN REACTION;

EID: 0031919331     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98245dt04     Document Type: Article

References (10)

1. Cotton, R.G.H. 1997. Slowly but surely towards better scanning for mutations. Trends Genet. 13:43-46.
2. Eng, C. and J. Vijg. 1997. Genetic testing: the problems and the promise. Nature Biotechnol. 15:422-426.
3. Hultman, T., S. Stahl, E. Hornes and M. Uhlén. 1989. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res. 17:4937-4946.
4. Leren, T.P., O.K. Rødningen, O. Røsby, K. Solberg and K. Berg. 1993. Screening for point mutations by semi-automated DNA sequencing using Sequenase® and magnetic beads. BioTechniques 14:618-623.
5. Parker, L.T., Q. Deng, H. Zakeri, C. Carlson, D.A. Nickerson and P.Y. Kwok. 1995. Peak height variations in automated sequencing of PCR products using Taq dye-terminator chemistry. BioTechniques 19:116-121.
6. Sourdaine, P., M.G. Parker, J. Telford and W.R. Miller. 1994. Analysis of the aromatase cytochrome P450 gene in human breast cancers. J. Mol. Endocrinol. 13:331-337.
7. Tabor, S. and C.C. Richardson. 1986. Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA polymerase I. Proc. Natl. Acad. Sci. USA 86:4076-4080.
8. Tabor, S. and C.C. Richardson. 1995. A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between deoxy- and dideoxyribonucleotides. Proc. Natl. Acad. Sci. USA 92:6339-6343.
9. Voss, H., C. Schwager, T. Kristensen, S. Duthie, A. Olsson, H. Erfle, J. Stegemann, J. Zimmermann and W. Ansorge. 1989. One-step reaction protocol for automated DNA sequencing with T7 DNA polymerase results in uniform labeling. Methods Mol. Cell. Biol. 1:155-159.
10. Voss, H., J. Stegemann, C. Schwager, J. Zimmermann, H. Erfle, N.A. Hewitt, T. Rupp and W. Ansorge. 1991. High-speed automated DNA fragment analysis for genome mapping by restriction fingerprinting. Methods Mol. Cell. Biol. 3:77-82.