Transposon Tn916 insertional mutagenesis of Pasteurella multocida and direct sequencing of disruption site

Microbial Pathogenesis

Volumn 24, Issue 4, 1998, Pages 203-209

  DeAngelis, Paul L ^a  

^a UNIVERSITY OF OKLAHOMA COLLEGE OF MEDICINE   (United States)

Author keywords

Electroporation; Mutagenesis; Pasteurella multocida; Tn916; Transposon

Indexed keywords

ARTICLE; BACTERIAL GENE; ELECTROPORATION; GENE AMPLIFICATION; GENE INSERTION; GENE MUTATION; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; PASTEURELLA MULTOCIDA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TRANSPOSON;

ANIMALIA; BACTERIA (MICROORGANISMS); NEGIBACTERIA; PASTEURELLA MULTOCIDA; PROKARYOTA;

EID: 0031918289     PISSN: 08824010     EISSN: None     Source Type: Journal    
DOI: 10.1006/mpat.1997.0189     Document Type: Article

References (21)

1. Nnalue NA, Stocker BA. Transfer and properties of some natural and suicide replicons in Pasteurella multocida. J Gen Microb 1989; 135: 3345-52.
2. Nnalue NA. Tn7 inserts in both orientations at a single chromosomal location and apparently forms co-integrates in Pasteurella multocida. Mol Microbiol 1990; 4: 107-17.
3. Lee MD, Henk AD. Tn10 insertional mutagenesis in Pasteurella multocida. Vet Microbiol 1996; 50: 143-8.
4. Gawron-Burke C, Clewell DB. Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia coli: strategy for targeting and cloning of genes from gram-positive bacteria. J Bact 1984; 159: 214-21.
5. Senghas E, Jones JM, Yamamoto M, Gawron-Burke C, Clewell DB. Genetic organization of the bacterial conjugative transposon Tn916. J Bact 1988; 170: 245-9.
6. Bertram J, Strätz M, Dürre P. Natural transfer of conjugative transposon Tn916 between Gram-positive and Gram-negative bacteria. J Bact 1991; 173: 443-8.
7. Franke AE, Clewell DB. Evidence for chromosomeborne resistance transposon in Streptococcus faecalis capable of 'conjugal' transfer in the absence of conjugative plasmid. J Bact 1981; 145: 494-502.
8. Kathariou S, Metz P, Hof H, Goebel W. Tn916 induced mutations in the hemolysin determinant affecting virulence of Listeria monocytogenes. J Bact 1987; 169: 1291-7.
9. Watson DA, Musher DM. Interruption of capsule production in Streptococcus pneumoniae serotype 3 by insertion of transposon Tn916. Infect Immun 1990; 58: 3135-8.
10. Kaue L, Goodgal SH. Introduction of transposon Tn916 DNA into Haemophilus influenzae and Haemophilus para-influenzae. J Bact 1989; 171: 6625-8.
11. Holland J, Towner KJ, Williams P. Tn916 insertion mutagenesis in Escherichia coli and Haemophilus influenzae type b following conjugative transfer. J Gen Microb 1992; 138: 509-15.
12. Scott JR. Sex and the single circle: conjugative transposition. J Bact 1992; 174: 6005-10.
13. Azad AK, Coote JG, Parton R. Construction of conjugative shuttle and suicide vectors for Pasteurella haemolytica and P. multocida. Gene 1994; 145: 81-5.
14. Jablonski L, Sriranganathan N, Bole SM, Carter GR. Conditions for transformation of Pasteurella multocida by electroporation. Microb Pathog 1992; 12: 63-8.
15. DeAngelis PL, Papaconstantinou J, Weigel PH. Molecular cloning, identification, and sequence of the hyaluronan synthase gene of Group A Streptococcus pyogenes. J Biol Chem 1993; 268: 19181-4.
16. Wilson MA, Morgan MJ, Barger GE. Comparison of DNA fingerprinting and serotyping for identification of avian Pasteurella multocida isolates. J Clin Microbiol 1993; 31: 255-9.
17. Fleischmann RD, Adams MD, White O, et al. Whole genome random sequencing and assembly of Haemophilus influenzae Rd. Science 1995; 269: 496-512.
18. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. 1989.
19. Norgren M, Scott JR. The presence of conjugative transposon Tn916 in the recipient strain does not impede transfer of a second copy of the element. J Bact 1991; 173: 319-24.
20. Wilson K. Preparation of genomic DNA from bacteria. In: Ausbel, FM, Brent R, Kingston RE, et al., Eds. Current Protocols in Molecular Biology. New York: Wiley Interscience Publishing, 1987: 2.4.1-2.4.5.
21. Ehtesham NZ, Hasnain SE. Direct in-gel hybridization without blotting, using nick-translated cloned DNA probe. BioTechniques 1991; 11: 718-21.