PCR-assisted cDNA cloning: A guided tour of the minefield

BioTechniques

Volumn 24, Issue 3, 1998, Pages 390-392

  Van Huijsduijnen, Rob Hooft ^a  

^a SERONO PHARMACEUTICAL RES INSTITUTE   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; DNA; DNA HYBRID;

ARTICLE; ARTIFACT; CONTAMINATION; GENE AMPLIFICATION; GENE EXPRESSION; MOLECULAR CLONING; MUTATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TISSUE DISTRIBUTION;

EID: 0031916299     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98243bm11     Document Type: Article

References (6)

1. Cline, J., J.C. Braman and H.H. Hogrefe. 1996. PCR fidelity of Pfu DNA polymerase and other thermostable DNA polymerases. Nucleic Acids Res. 24:3546-3551.
2. Eckert, K.A. and T.A. Kunkel. 1990. High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Nucleic Acids Res. 18:3739-3744.
3. Erlich, H.A., D. Gelfand and J.J. Sninsky. 1991. Recent advances in the polymerase chain reaction. Science 252:1643-1651.
4. Hooft van Huijsduijnen, R. 1998. ADAM 20 and 21; two novel human testis-specific membrane metalloproteases with similarity to fertilin-α. Gene 206:273-282.
5. Kenzelmann, M. and K. Mühlemann. 1997. Pitfalls of PCR: cross-reactivity with joyride E. coli nucleic acid. BioTechniques 23:204-206.
6. Wolfsberg, T.G., P.D. Straight, R.L. Gerena, A.P. Huovila, P. Primakoff, D.G. Myles and J.M. White. 1995. ADAM, a widely distributed and developmentally regulated gene family encoding membrane proteins with a disintesrin and metalloprotease domain. Dev. Biol. 169:378-383.