Purification and characterization of the heteromeric transcriptional activator MvaT of the Pseudomonas mevalonii mvaAB operon

Protein Science

Volumn 7, Issue 1, 1998, Pages 178-184

  Rosenthal, R Scott ^a,b   Rodwell, Victor W ^a  

^a PURDUE UNIVERSITY   (United States)

^b UNIVERSITY OF COLORADO   (United States)

Author keywords

Bacterial transcriptional activation; Heterodimer; Mevalonate catabolism; MvaAB; Pseudomonas mevalonii

Indexed keywords

MEVALONIC ACID;

ARTICLE; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME PURIFICATION; NONHUMAN; OPERON; PRIORITY JOURNAL; PSEUDOMONAS; TRANSCRIPTION INITIATION;

PSEUDOMONAS MEVALONII;

EID: 0031915442     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560070118     Document Type: Article

References (30)

1. Beach MJ. 1987. Mevalonate catabolism in Pseudomonas: Cloning and sequencing HMG-CoA reductase [thesis]. West Lafayette, Indiana: Purdue University.
2. 31P Nuclear magnetic resonance spectra and dissociation constants of lac repressor headpiece-duplex operator complexes: The importance of phosphate ester backbone flexibility in protein-DNA recognition. Biochemistry 32:6863-6874.
3. Buggy JJ, Sganga MW, Bauer CE. 1994. Nucleotide sequence and characterization of the Rhodobacter capsulatus hvrB gene: HvrB is an activator of S-adenosyl-L-homoserine hydrolase expression and is a member of the LysR family. J Bacteriol 176:61-69.
4. Chou PY, Fasman GD. 1978. Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol 47:45-147.
5. Devereux J, Haeberli P, Smithes O. 1984. A comprehensive set of sequence analysis programs for the Vax. Nucleic Acids Res 12:387-395.
6. D'Orazio SEF, Collins CM. 1993. The plasmid-encoded urease gene cluster of the family Enterobacteriaceae is positively regulated by UreR, a member of the AraC family of transcriptional activators. J Bacteriol 175:3459-3467.
7. Dunker AK, Rueckert RR. 1969. Observations on molecular weight determinations on polyacrylamide gels. J Biol Chem 244:5074-5080.
8. Fisher RF, Long SR. 1993. Interactions of NodD at the nod box: NodD binds to two distinct sites on the same face of the helix and induces a bend in the DNA. J Mol Biol 233:336-348.
9. Freemont PS, Lane AN, Sanderson MR. 1991. Structural aspects of protein-DNA recognition. Biochem J 278:1-23.
10. Gill JF Jr, Beach MJ, Rodwell VW. 1985. Mevalonate utilization in Pseudomonas sp. M. Purification and characterization of an inducible 3-hydroxy-3-methylglutaryl coenzyme A reductase. J Biol Chem 260:9393-9398.
11. Harrison SC. 1991. A structural taxonomy of DNA-binding domains. Nature 353:715-719.
12. Kadonaga JT, Tjian R. 1986. Affinity purification of sequence-specific DNA binding proteins. Proc Natl Acad Sci USA 83:5889-5893.
13. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680.
14. Lavigne M, Kolb A, Buc H. 1992. Transcription activation by cAMP receptor protein (CRP) at the Escherichia coli gal P1 promoter. Crucial role for the spacing between the CRP binding site and the - 10 region. Biochemistry 31:9647-56.
15. Li Z, Demple B. 1994. SoxS, an activator of superoxide stress genes in Escherichia coli: Purification and interaction with DNA. J Biol Chem 269:18371-18377.
16. Mak YM, Ho KK. 1991. An improved method for the isolation of chromosomal DNA from various bacteria and cyanobacteria. Nucleic Acids Res 20:4101-4102.
17. Parsek MR, Shinabarger DL, Rommel RK, Chakrabarty AM. 1992. Roles of CatR and cis, cis-muconale in activation of the catBC operon, which is involved in benzoate degradation in Pseudomonas putida. J Bacteriol 174:7798-7806.
18. Parthun MR, Jaehning JA. 1990. Purification and characterization of the yeast transcriptional activator GAL4. J Biol Chem 265:209-213.
19. Rodwell VW, Anderson DH, Beach MJ, Gill JF Jr, Jordan-Starck TC, Scher DS, Wang Y. 1990. Organization and regulation of the mva operon in Pseudomonas mevalonii. In: Silver S, Chakrabarty AM, Ingelewski B, Kaplan S, eds. Pseudomonas: Biotransformations, pathogenesis, and evolving technology. Washington, DC: ASM Press, pp 151-158.
20. Rothmel RK, Chakrabarty AM, Berry A, Darzins A. 1991. Genetic systems in Pseudomonas. Methods Enzymol 204:485-514.
21. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: A laboratory manual, 2nd ed. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
22. Sanger F, Nicklen S, Coulson AR. 1977. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463-5467.
23. Schell MA. 1993. Molecular biology of the LysR family of transcriptional regulators. Annu Rev Microbiol 47:597-626.
24. Schweizer HP. 1991. The agmR gene, an environmentally responsive gene, complements defective glpR, which encodes the putative activator for glycerol metabolism in Pseudomonas aeruginosa. J Bacteriol 173:6798-6806.
25. Slock JB, Ninfa AJ, Stock A. 1989. Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol Rev 53:450-490.
26. Wang Y. 1990. Pseudomonas mevalonii HMG-CoA reductase: Gene regulation and catalytic mechanism [thesis]. West Lafayette, Indiana: Purdue University.
27. Wang Y, Beach MJ, Rodwell VW. 1989. (S)-3-Hydroxy-3-methylglutaryl coenzyme A reductase, a product of the mva operon of Pseudomonas mevalonii, is regulated at the transcriptional level. J Bacteriol 171:5567-5571.
28. Wang Y, Rodwell VW. 1991. The cis-acting regulatory element of the mvaAB operon of Pseudomonas mevalonii. J Bacteriol 173:3803-3806.
29. Wasenius V, Merilainen J, Lehto V. 1993. Sequences of a chicken cDNA encoding a GRB2 protein. Gene 134:299-300.
30. Wilson S, Drew R. 1991. Cloning and DNA sequence of amiC, a new gene regulating expression of the Pseudomonas aeruginosa aliphatic amidase, and purification of the amiC product. J Bacteriol 173:4914-4921.