The acetylcholinesterase ichthyotoxin is a common component in the extracellular products of Vibrionaceae strains

Journal of Applied Microbiology

Volumn 84, Issue 1, 1998, Pages 47-52

  Perez M J ^a   Rodriguez L A ^a   Nieto, T P ^a  

^a UNIVERSITY OF VIGO   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; FISH; TOXIC MATERIALS;

ACETYLCHOLINESTERASE; ACETYLCHOLINESTERASE ACTIVITY; AEROMONAS; AEROMONAS HYDROPHILA; COLORIMETRIC METHODS; EXTRACELLULAR; IMMUNOLOGICAL METHODS; QUALITATIVE METHOD; VIBRIONACEAE; WESTERN BLOTS;

STRAIN;

ACETYLCHOLINESTERASE; BACTERIAL TOXIN; FISH VENOM; NEUROTOXIN;

AEROMONAS HYDROPHILA; ARTICLE; BACTERIAL VIRULENCE; ENZYME SPECIFICITY; LETHALITY; NEUROTOXICITY; NONHUMAN; STRAIN DIFFERENCE; TOXIN ANALYSIS; TOXIN SYNTHESIS;

AEROMONAS; AEROMONAS HYDROPHILA; BACTERIA (MICROORGANISMS); NEGIBACTERIA; VIBRIO; VIBRIONACEAE;

EID: 0031915332     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2672.1997.00311.x     Document Type: Article

References (22)

1. Angulo, L., López, J.L., Lema, C. and Vicente, J.A. (1992) Vibrio pelagius associated with mortalities in farmed turbot, Scophthalmus maximus. Thalasas 10, 129-133.
2. Angulo, L., López, L.A. and Vicente, J.A. (1994a) Epizootic outbreaks by a protozoan and a bacterium in reared turbot (Scophthalmus maximus). Bulletin of European Association of Fish Pathalogists 14, 39-40.
3. Angulo, L., López, J.E., Vicente, J.A. and Saborido, A.M. (1994b) Haemorrhagic areas in the mouth of farmed turbot, Scophthalmus maximus (L.). Journal of Fish Diseases 17, 163-169.
4. Balebona, M.C. (1994) Caracterización y Mecanismos de Virulencia de Cepas del Género Vibrio Aisladas de Patologías de Doradas (Sparatus aurata, L.) Cultivadas. PhD thesis, University of Malaga, Malaga, Spain.
5. Bradford, N.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-dye binding. Analytical Biochemistry 72, 248-254.
6. Bryant, T.N., Lee, J.V., West, P.A. and Colwell, R.R. (1986) Numerical classification of species of Vibrio and related genera. Journal of Applied Bacteriology 61, 437-467.
7. Hastings, T.S. and Ellis, A.E. (1988) The humoral immune response of rainbow trout, Salmo gairdneri, Richardson, and rabbit to Aeromonas salmonicida extracellular products. Journal of Fish Diseases 11, 147-160.
8. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacateriophage T4. Nature (London) 227, 680-685.
9. Liu, P.V. (1957) Survey of hemolysin production among species of pseudomonads. Journal of Bacteriology 74, 718-727.
10. Nieto, T.P., Corcobado, M.J.R., Toranzo, A.E. and Barja, J.L. (1985) Relation of water temperature to infection of Salmo gairdneri with motile Aeromonas. Fish Pathology 20, 99-105.
11. Nieto, T.P. and Ellis, A.E. (1991) Heterogeneity of extracellular proteases produced by different isolates of Aeromonas hydrophila and A. sobria pathogenic for fish. Journal of Fish Diseases 14, 229-235.
12. Nieto, T.P., Santos, Y., Rodríguez, L.A. and Ellis, A.E. (1991) An extracellular acetylcholinesterase produced by Aeromonas hydrophila is a major lethal toxin for fish. Microbial Pathogenesis 11, 101-110.
13. Popoff, M. (1984) Genus III. In: Bergey's Mannual of Determinative Bacteriology, 9th edn. ed. Holt J.G.and Krieg, N.R. pp. 545-548. Baltimore, MD: Williams & Wilkins Co.
14. Rappaport, F., Fischl, J. and Pinto, N. (1959) An improved method for the estimation of cholinesterase activity in serum. Clinica et Chimica Acta 4, 227.
15. Reed, L.J. and Müench, H. (1938) A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27, 493-497.
16. Rodriguez, L.A., Ellis, A.E. and Nieto, T.P. (1993a) Effects of the acetylcholinesterase toxin of Aeromonas hydrophila on the central nervous system of fish. Microbial Pathogenesis 14, 411-415.
17. 32: all are lethal for fish. Microbial Pathogenesis 13, 17-24.
18. Rodríguez, L.A., Fernández, A.I.G. and Nieto, T.P. (1993b) Production of the lethal acetylcholinesterase toxin by different Aeromonas hydrophila strains. Journal of Fish Diseases 16, 73-78.
19. Rodriguez, L.A., Fernández, A.I.G., Santos, V. and Nieto, T.P. (1990) Caracterización de una Toxina de A. hydrophila Letal para Peces ed. Land'n, A. and Cerviño, A. pp. 779-783. Acta III Congreso Nacional Acuicultura, Conseller'a de Pesca, Marisqueo e Acuicultura, Xunta de Galicia.
20. Santos, Y. (1991) Factores de Virulencia y Caracteristicas Antigénicas de Vibrio anguillarum y Aeromonas Móviles. PhD thesis, University of Santiago de Compostela, Spain.
21. Santos, Y., Toranzo, A.E., Barja, J.L., Nieto, T.P. and Villa, T.G. (1988) Virulence properties and enterotoxin production of Aeromonas strains isolated from fish. Infection and Immunity 56, 3285-3293.
22. West, P. and Colwell, R.R. (1984) Identification and classification of Vibrionaceae. An overview. In Vibrios in the Environment ed. Colwell, R.R. pp. 285-363. Chichester, UK: John Wiley & Sons.