Purification and characterization of a [3Fe-4S] [4Fe-4S] type ferredoxin from hyperthermophilic archaeon, Pyrobaculum islandicum

Journal of Biochemistry

Volumn 123, Issue 3, 1998, Pages 521-527

  Nakajima, Yasuhiro ^a   Fujiwara, Taketomo ^a   Fukumori, Yoshihiro ^b  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^b KANAZAWA UNIVERSITY   (Japan)

Author keywords

Ferredoxin; Hyperthermophile; Iron sulfur cluster; Pyrobaculum islandicum

Indexed keywords

2 OXOGLUTARIC ACID; BACTERIAL PROTEIN; FERREDOXIN; METAL; OXIDOREDUCTASE;

AMINO ACID SEQUENCE; ARCHAEBACTERIUM; ARTICLE; ELECTRON SPIN RESONANCE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN PURIFICATION; REDUCTION;

ARCHAEA; BACTERIA (MICROORGANISMS); PYROBACULUM ISLANDICUM;

EID: 0031915266     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021967     Document Type: Article

References (33)

1. Mukund, S. and Adams, M.W.W. (1991) The novel tungsteniron-sulfur protein of the hyperthermophilic archaebacterium, Pyrococcus furiosus, is an aldehyde ferredoxin oxidoreductase. J. Biol. Chem. 266, 14208-14216
2. Johnson, J.L., Rajagopalan, K.V., Mukund, S., and Adams, M.W.W. (1993) Identification of molybdopterin as the organic component of the tungsten cofactor in four enzymes from hyperthermophilic Archaea. J. Biol. Chem. 268, 4848-4852
3. Mukund, S. and Adams, M.W.W. (1993) Characterization of a novel tungsten-containing formaldehyde ferredoxin oxidoreductase from the hyperthermophilic archaeon, Thermococcus litoralis. J. Biol. Chem. 268, 13592-13600
4. Mukund, S. and Adams, M.W.W. (1995) Glyceraldehyde-3-phosphate ferredoxin oxidoreductase, a novel tungsten-containing enzyme with a potential glycolytic role in the hyperthermophilic archaeon Pyrococcus furiosus. J. Biol. Chem. 270, 8389-8392
5. Ma, K., Schicho, R.N., Kelly, R.M., and Adams, M.W.W. (1993) Hydrogenase of the hyperthermophile Pyrococcus furiosus is an elemental sulfur reductase or sulfhydrogenase: Evidence for a sulfur-reducing hydrogenase ancestor. Proc. Natl. Acad. Sci. USA 90, 5341-5344
6. Ma, K. and Adams, M.W.W. (1994) Sulfide dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus: A new multifunctional enzyme involved in the reduction of elemental sulfur. J. Bacteriol. 176, 6509-6517
7. Bryant, F.O. and Adams, M.W.W. (1989) Characterization of hydrogenase from the hyperthermophilic archaebacterium, Pyrococcus furiosus. J. Biol. Chem. 264, 5070-5079
8. Ma, K., Zhou, Z.H., and Adams, M.W.W. (1994) Hydrogen production from pyruvate by enzymes purified from the hyperthermophilic archaeon, Pyrococcus furiosus: A key role for NADPH. FEMS Microbiol. Lett. 122, 245-250
9. Iwasaki, T., Wakagi, T., and Oshima, T. (1995) Ferredoxin-dependent redox system of a thermoacidophilic archaeon, Sulfolobus sp. strain 7. J. Biol. Chem. 270, 17878-17883
10. Zhang, Q., Iwasaki, T., Wakagi, T., and Oshima, T. (1996) 2-Oxoacid: ferredoxin oxidoreductase from the thermoacidophilic archaeon, Sulfolobus sp. strain 7. J. Biochem. 120, 587-599
11. Fujii, T., Hata, Y., Wakagi, T., Tanaka, N., and Oshima, T. (1996) Novel zinc-binding centre in thermoacidophilic archaeal ferredoxins. Nature Struct. Biol. 3, 834-837
12. Fujii, T., Hata, Y., Oozeki, M., Moriyama, H., Wakagi, T., Tanaka, N., and Oshima, T. (1997) The crystal structure of zinc-containing ferredoxin from the thermoacidophilic archaeon Sulfolobus sp. strain 7. Biochemistry 36, 1505-1513
13. Iwasaki, T., Suzuki, T., Kon, T., Imai, T., Urushiyama, A., Ohmori, D., and Oshima, T. (1997) Novel zinc-containing ferredoxin family in thermoacidophilic archaea. J. Biol. Chem. 272, 3453-3458
14. Schauder, R. and Kroger, A. (1993) Bacterial sulphur respiration. Arch. Microbiol. 159, 491-497
15. 2 with sulfur or thiosulfate as electron acceptor in the anaerobic hyperthermophilic archaea Thermoproteus tenax and Pyrobaculum islandicum proceeds via the citric acid cycle. Arch. Microbiol. 162, 286-294
16. Huber, R., Kristjansson, J.K., and Stetter, K.O. (1987) Pyrobaculum gen. nov., a new genus of neutrophilic, rod-shaped archaebacteria from continental solfataras growing optimally at 100°C. Arch. Microbiol. 149, 95-101
17. Schägger, H.J. and von Jacow, G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal.Biochem. 166, 368-379
18. Kakuno, T. (1994) HPLC gel nitration in Theory and Practice on Enzymes and Other Proteins (Horio, T., ed.) pp. 241-254, Nankodo, Tokyo
19. Crestfield, A.M., Moore, S., and Stein, W.H. (1963) The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins. J. Biol. Chem. 238, 622-627
20. Inglis, A.S. (1983) Cleavage at aspartic acid. Methods Enzymol. 91, 324-332
21. Wellner, D., Panneerselvam, C., and Horecker, B.L. (1990) Sequencing of peptides and proteins with blocked N-terminal amino acids:N-acetylserine or N-acetylthreonine. Proc. Natl. Acad. Sci. USA 87, 1947-1949
22. Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354
23. Trower, M.K., Emptage, M.H., and Sariaslani, F.S. (1990) Purification and characterization of a 7Fe ferredoxin from Streptomyces griseus. Biochim. Biophys. Acta 1037, 281-289
24. Johnson, M.K., Bennett, D.E., Fee, J.A., and Sweeney, W.V. (1987) Spectroscopic studies of the seven-iron-containing ferredoxins from Azotobacter vinelandii and Thermus thermophilus. Biochim. Biophys. Acta 911, 81-94
25. Iwasaki, T., Wakagi, T., Isogai, Y., Tanaka, K., Iizuka, T., and Oshima, T. (1994) Functional and evolutionary implications of a [3Fe-4S] cluster of the dicluster-type ferredoxin from the thermoacidophilic archaeon, Sulfolobus sp. strain 7. J. Biol. Chem. 269, 29444-29450
26. Armstrong, F.A., George, S.J., Cammack, R., Hatchikian, E.C., and Thomson, A.J. (1989) Electrochemical and spectroscopic characterization of the 7Fe form of ferredoxin III from Dsulfovibrio africanus. Biochem. J. 264, 265-273
27. Adams, M.W.W. (1992) Novel iron-sulfur centers in metalloenzymes and redox proteins from extremely thermophilic bacteria. Adv. Inorg. Chem. 38, 341-396
28. Aono, S., Bryant, F.O., and Adams, M.W.W. (1989) A novel and remarkably thermostable ferredoxin from the hyperthermophilic archaebacterium Pyrococcus furiosus. J. Bacteriol. 171, 3433-3439
29. Blamey, J.M. and Adams, M.W.W. (1993) Purification and characterization of pyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus. Biochim. Biophys. Acta 1161, 19-27
30. Schicho, R.N., Ma, K., Adams, M.W.W., and Kelly, R.M. (1993) Bioenergetics of sulfur reduction in the hyperthermophilic archaeon Pyrococcus furiosus J. Bacteriol. 175, 1823-1830
31. Orme-Johnson, W.H. and Sands, R.H. (1973) Probing iron-sulfur proteins with EPR and ENDOR spectroscopy in Iron-Sulfur Proteins (Lovenberg, W., ed.) Vol. 2, pp. 195-238, Academic Press, New York
32. Malkin, R. (1973) The reconstitution of clostridial ferredoxin in Iron-Sulfur Proteins (Lovenberg, W., ed.) Vol. 2, pp. 1-26, Academic Press, New York
33. Hulmes, J.D. and Pan, Y.-C.E. (1991) Selective cleavage of polypeptides with trifluoroacetic acid: Applications for microsequencing. Anal. Biochem. 197, 368-376