Immunoaffinity purification of antibodies against GST fusion proteins

BioTechniques

Volumn 24, Issue 2, 1998, Pages 198-202

  Youssoufian, Hagop ^a,b  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HYBRID PROTEIN; POLYCLONAL ANTIBODY;

ANTIBODY AFFINITY; ANTIBODY DETECTION; ARTICLE; COLONY FORMATION; COVALENT BOND; IMMUNOGENICITY; NONHUMAN; PEPTIDE SYNTHESIS; PROTEIN PURIFICATION; TARGET CELL;

EID: 0031914367     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98242bm05     Document Type: Article

References (4)

1. Frangioni, J.V. and B.G. Neel. 1993. Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins. Anal. Biochem. 210:179-187.
2. Harlow, E. and D. Lane. 1988. Antibodies. CSH Laboratory Press, Cold Spring Harbor, NY.
3. Youssoufian, H. 1994. Localization of Fanconi anemia C protein to the cytoplasm of mammalian cells. Proc. Natl. Acad. Sci. USA 91:7975-7979.
4. Youssoufian, H. 1996. Cytoplasmic localization of FAC is essential for the correction of a prerepair defect in Fanconi anemia group C cells. J. Clin. Invest. 97:2003-2010.