Production of milligram concentrations of free prostate specific antigen (fPSA) from LNCaP cell culture: Difference between fPSA from LNCaP cell and seminal plasma

Journal of Clinical Laboratory Analysis

Volumn 12, Issue 1, 1998, Pages 6-13

  Wu, James T ^a,b   Lyons, Bradley W ^a   Liu, Grace H ^a   Wu, Lily L ^a  

^a ARUP LABORATORIES   (United States)

^b UNIVERSITY OF UTAH HEALTH SCIENCES CENTER   (United States)

Author keywords

Bioreactor; Chromatofocusing; DEAE sepharose chromatography; Protease activity; PSA ACT complex; Sephacryl gel chromatography; Western blot

Indexed keywords

ALPHA 1 ANTICHYMOTRYPSIN; BOVINE SERUM ALBUMIN; METRIBOLONE; PROSTATE SPECIFIC ANTIGEN;

ANTIGEN DETECTION; ARTICLE; ASSAY; CALIBRATION; CELL CULTURE; CONTROLLED STUDY; GEL CHROMATOGRAPHY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; MALE; PROSTATE CARCINOMA; PROSTATE HYPERTROPHY; SEMINAL PLASMA;

BOVINAE;

EID: 0031914213     PISSN: 08878013     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2825(1998)12:1<6::AID-JCLA2>3.0.CO;2-A     Document Type: Article

References (19)

1. Oesterling JE: Prostate-specific antigen. Improving its ability to diagnose early prostate cancer. JAMA (Editorial) 267:2236-2238, 1992.
2. Wang MC, Papsidero LD, Kuriyama M, et al.: Prostate antigen: A new potential marker for prostatic cancer. Prostate 2:89-96, 1981.
3. Wu JT: Assay for prostate specific antigen (PSA): Current problems and possible solutions. J Clin Lab Anal 8:51-62, 1994.
4. Wu JT, Zhang P, Wang T, et al.: Evaluation of free PSA isoforms, PSA complex formation and specificity of anti-PSA antibodies by HPLC and PAGE-immunoblotting techniques. J Clin Lab Anal 9:1-14, 1995.
5. Catalona W, Smith D, Ratliff T, et al.: Measurement of prostate-specific antigen in serum as a screening test for prostate cancer. N Engl J Med 324:1156-1161, 1991.
6. Catalona WJ, Smith DS, Wolfert RL, et al.: Evaluation of percentage of free serum prostate-specific antigen to improve specificity of prostate cancer screening. JAMA 274:1214-1220, 1995.
7. Wu JT, Zhang P, Bandhauer ME, et al.: Purification of PSA-ACT complex: characterization of PSA-ACT complex by various chromatographic procedures. J Clin Lab Anal 9:25-31, 1995.
8. Wu JT, Wilson L, Zhang P, et al.: Correlation of serum concentrations of PSA-ACT complex with total PSA in random and serial specimens from patients with BPH and prostate cancer. 9:15-24, 1995.
9. Gleave ME, Hsieh J-T, Wu H-C, et al.: Serum prostate specific antigen levels in mice bearing human prostate LNCaP tumors are determined by tumor volume and endocrine and growth factors. Cancer Res 52:1598-1605, 1992.
10. Gleave ME, Hsieh J-T, von Eschenbach AC, Chung LWK: Prostate and bone fibroblasts induce human prostate cancer growth in vivo: Implications for bi-directional tumor-stromal cell interaction in prostate carcinoma growth and metastasis. J Urol 147:1151-1159, 1992.
11. Langeler EG, van Uffelen CJC, Blankenstein MA, et al.: Effect of culture conditions on androgen sensitivity of the human prostatic cancer cell line LNCaP. Prostate 23:213-223, 1993.
12. Hsieh J-T, Wu H-C, Gleave ME. et al.: Autocrine retulation of prostate-specific antigen gene expression in a human prostatic cancer (LNCaP) subline. Cancer Res 53:2852-2857, 1993.
13. Esquenet M, Swinnen JV, Heyns, Verhoeven G: Control of LNCaP proliferation and differentiation: Actions and interactions of androgens, 1α,25-dihydroxycholecalciferal, all-trans retinoic acid, 9-cis retinoic acid, and phenylacetate. Prostate 28:182-194, 1996.
14. Wu JT, Zhang P, Wang T, et al.: Evaluation of free PSA isoforms, PSA complex formation and specificity of anti-PSA antibodies by HPLC and PAGE-immunoblotting techniques. J Clin Lab Anal 9:1-14, 1995.
15. Wu JT, Wilson LW: Development of a microplate ELISA for free PSA and PSA-ACT complex in serum. J Clin Lab Anal 9:252-260, 1995.
16. Wu JT, Stephenson RA: Microplate assay for free PSA, PSA-ACT and total PSA: Assay characteristics, selection of calibrators and in vitro stability studies. Proceeding for the 1st International Consultation on Prostate Cancer, 1997, p 159-168.
17. Wu JT, Zhang P, Astill ME, et al.: PSA immunoreactivity detected in LNCaP cell medium, breast tumor cytosol and female serum. J Clin Lab Anal 9:243-251, 1995.
18. Wu JT, Liu GH, Zhang P: Effect of denaturation and curve fitting on the accuracy of microplate fPSA assay (submitted).
19. Wu JT, Liu GH, Zhang P, Stephenson RA: Determination of percent free prostate-specific antigen may identify occult prostate tumor and provide early detection. (submitted).