Flow cytometric apoptosis assays indicate different types of endonuclease activity in haematopoietic cells and suggest a cautionary approach to their quantitative use

Cytometry

Volumn 31, Issue 2, 1998, Pages 130-136

  McKenna, S L ^a   Hoy, T ^b   Holmes, J A ^c   Whittaker, J A ^b   Jackson, H ^b   Padua, R A ^d  

^a UNIVERSITY COLLEGE CORK   (Ireland)

^b CARDIFF UNIVERSITY   (United Kingdom)

^c QUEEN ELIZABETH HOSPITAL   (United Kingdom)

^d SAINT LOUIS HOSPITAL   (France)

Author keywords

Apoptosis; Chronic lymphocytic leukaemia (CLL); Endonuclease; Flow cytometry; ISNT; TdT

Indexed keywords

DEOXYURIDINE TRIPHOSPHATE DERIVATIVE; DNA; DNA NUCLEOTIDYLEXOTRANSFERASE; ENDONUCLEASE; ETOPOSIDE; EXONUCLEASE; NUCLEOTIDE;

APOPTOSIS; ARTICLE; CHRONIC LYMPHATIC LEUKEMIA; CONTROLLED STUDY; DNA CONTENT; DNA STRAND BREAKAGE; ENZYME ACTIVITY; FLOW CYTOMETRY; HEMATOPOIETIC CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL;

APOPTOSIS; CELLS, CULTURED; DEOXYRIBONUCLEASE I; DEOXYURACIL NUCLEOTIDES; DNA NUCLEOTIDYLEXOTRANSFERASE; DNA POLYMERASE I; DNA PROBES; FLOW CYTOMETRY; HL-60 CELLS; HUMANS; LEUKEMIA, LYMPHOCYTIC, CHRONIC; LEUKOCYTES, MONONUCLEAR;

EID: 0031914028     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19980201)31:2<130::AID-CYTO8>3.0.CO;2-Q     Document Type: Article

References (30)

1. Alnemri ES, Litwack G: Activation of internucleosomal DNA cleavage in human CEM lymphocytes by glucocorticoid and novobiocin. J Biol Chem 265:17323-17333, 1990.
2. + cells and their granulocytic descendants. Blood 86:917-923, 1995.
3. Barry MA, Eastman A: Identification of deoxyribonuclease II as an endonuclease involved in apoptosis. Arch Biochem Biophys 300:440-450, 1993.
4. Begleiter A, Lee K, Israels LG, Mowat MRA, Johnstone JB: Chlorambucil induced apoptosis in chronic lymphocytic leukemia (CLL) and its relationship to clinical efficacy. Leukemia 8 (Suppl 1):S103-S106, 1994.
5. Bernardi G: Spleen acid deoxyribonuclease. In: The Enzymes, Ed. 3, Vol. 4, Boyer PD (ed). Academic Press, New York, 1971, pp 271-287.
6. Carbonari M, Cibati M, Fiorilli M: Measurement of apoptotic cells in peripheral blood. Cytometry 22:161-167, 1995.
7. Chang LM, Bollum FJ: Molecular biology of terminal transferase. CRC Crit Rev Biochem 21:27-52, 1986.
8. Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, Traganos F: Features of apoptotic cells measured by flow cytometry. Cytometry 13:795-808, 1992.
9. Deng G, Podack ER: Deoxyribonuclease induction in apoptotic cytotoxic T lymphocytes. FASEB. J. 9:665-669, 1995.
10. Deng G-R, Wu R: Terminal transferase: Use in the tailing of DNA and for in vitro mutagenesis. Methods Enzymol 100:96-116, 1983.
11. Funk A, Rudel J, Fellbaum C, Hofler H: Specific in situ labeling of apoptosis shows different rates of programmed cell death in non-Hodgkin lymphomas (original title: spezifische in situ markierung von apoptosen zeigt unterschiedliche raten von programmiertem zelltod in Non Hodgkin lymphomen). Verh Dtsch Ges Pathol 78:318-320, 1994.
12. Gorczyca W, Bigman K, Mittelman A, Ahmed T, Gong J, Melamed MR, Darzynkiewicz Z: Induction of DNA strand breaks associated with apoptosis during treatment of leukaemias. Leukemia 7:659-670, 1993.
13. Gorczyca W, Gong J, Darzynkiewicz Z: Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res. 53:1945-1951, 1993.
14. Gorczyca W, Melamed MR, Darzynkiewicz Z: Apoptosis of S-phase HL60 cells induced by DNA topoisomerase II inhibitors: Detection of DNA strand breaks by flow cytometry using the in situ nick translation assay. Toxicol. Lett. 67:249-258, 1993.
15. Gottlieb RA, Giesing HA, Engler RL, Babior BM: The acid deoxyribonuclease of neutrophils: A possible participant in apoptosis-associated genome destruction. Blood 86:2414-2418, 1995.
16. Jackson DA, Symons RH, Berg P: Biochemical method for inserting new genetic information into DNA of Simian virus 40: Circular SV 40 DNA molecules containing Lambda phage genes and the galactose operon of Escherichia coli. P.N.A.S. 69:2904-2909, 1972.
17. Kornberg T, Kornberg A: Bacterial DNA polymerases. Vol. X. In: The Enzymes, Boyer PD (ed). Academic Press, London, 1974, pp 119-144.
18. Laskowski M: Deoxyribonuclease I. In: The Enzymes, Ed. 3, Vol. 4, Boyer PD (ed). Academic Press, New York, 1971, pp 289-311.
19. Little JW: An exonuclease induced by bacteriophage Lambda. Nature of the enzymatic reaction. J. Biol. Chem. 242:679-686, 1967.
20. Lobban PE, Kaiser AD: Enzymatic end-to-end joining of DNA molecules. J. Mol. Biol. 78:453-471, 1973.
21. Matsubara K, Kubota M, Adachi S, Kuwakado K, Hirota H, Wakazono Y, Akiyama Y, Mikawa H: Different mode of cell death induced by calcium ionophore in human leukemia cell lines: Possible role of constitutive endonuclease. Exp. Cell Res. 210:19-25, 1994.
22. Michelson AM, Orkin SH: Characterization of the homopolymer tailing reaction catalysed by terminal deoxynucleotidyl transferase. J. Biol. Chem. 257:14773-14782, 1982.
23. Peitsh MC, Polzar B, Stephan H, Crompton T, MacDonald HR, Mannherz HG, Tschopp J: Characterization of the endogenous deoxyribonuclease involved in nuclear DNA degradation during apoptosis (programmed cell death). EMBO J. 12:371-377, 1993.
24. Robertson LE, Plunkett W: Apoptotic cell death in chronic lymphocytic leukaemia. Leuk. Lymph 11 (Suppl 2):71-74, 1993.
25. Robertson LE, Chubb S, Meyn RE, Story M, Ford R, Hittelman WN, Plunkett W: Induction of apoptotic cell death in chronic lymphocytic leukaemia by 2-chloro-2′-deoxyadenosine and 9-beta-D-arabinosyl-2-Fluoroadenine. Blood 81:143-150, 1993.
26. Roychoudhury R, Jay E, Wu R: Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res. 3:863-877, 1976.
27. Sgonc R, Boeck G, Dietrich H, Gruber J, Recheis H, Wick G: Simultaneous determination of cell surface antigens and apoptosis. TIG 10:41-42, 1994.
28. Tanuma S, Shiokawa D: Multiple forms of nuclear deoxyribonuclease in rat thymocytes. Biochem. Biophys. Res. Commun. 203:789-797, 1994.
29. Wyllie AH, Arends MJ, Morris RG, Walker SW, Evans G: The apoptosis endonuclease and its regulation. Semin Immunol. 4:389-397, 1992.
30. Yanagisawa-Shiota F, Sakagami H, Kuribayashi N, Iida M, Sakagami T, Takeda M: Endonuclease activity and induction of DNA fragmentation in human myelogenous leukemic cell lines. Anticancer Res. 15:259-265, 1995.