Investigation of the potential active site of a cyanide dihydratase using site-directed mutagenesis

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1382, Issue 1, 1998, Pages 1-4

  Watanabe, Atsushi ^a   Yano, Kazuyoshi ^a   Ikebukuro, Kazunori ^a   Karube, Isao ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Active site; Cyanide dihydratase; Enzyme activity; Nitrilase; Site directed mutagenesis

Indexed keywords

AMINO ACID; CYANIDE; CYANIDE DIHYDRATASE; CYSTEINE; HYDROLYASE; NITRILASE; PHENYLALANINE; SERINE; TYROSINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVE SITE; HYDROLYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PSEUDOMONAS STUTZERI; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BASE SEQUENCE; BINDING SITES; CYSTEINE; HYDROLASES; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OLIGODEOXYRIBONUCLEOTIDES; PHENYLALANINE; PSEUDOMONAS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SERINE; TYROSINE;

PSEUDOMONAS; PSEUDOMONAS STUTZERI;

EID: 0031913587     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(97)00189-1     Document Type: Article

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