Purification and characterization of two fibrinolytic enzymes from a marine green alga, Codium intricatum

Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

Volumn 119, Issue 1, 1998, Pages 177-181

  Matsubara, Kiminori ^a   Sumi, Hiroyuki ^a,c   Hori, Kanji ^b   Miyazawa, Keisuke ^b  

^a OKAYAMA PREFECTURAL UNIVERSITY   (Japan)

^b HIROSHIMA UNIVERSITY   (Japan)

^c KURASHIKI UNIVERSITY OF SCIENCE AND THE ARTS   (Japan)

Author keywords

Codium intricatum; Fibrinogenolytic activity; Fibrinolytic activity; Marine alga; Molecular weight; Optimum pH; Protease activity; Purification; Serine protease

Indexed keywords

PLASMIN; PROTEINASE; SERINE PROTEINASE;

ALGA; ARTICLE; ENZYME ANALYSIS; ENZYME PURIFICATION; FIBRINOLYSIS; GEL FILTRATION; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; STRAIN DIFFERENCE;

ALGAE; CHLOROPHYTA; CODIUM DIVARICATUM; CODIUM FRAGILE; CODIUM INTRICATUM; CODIUM PUGNIFORMIS; EUKARYOTA;

EID: 0031913551     PISSN: 03050491     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0305-0491(97)00303-9     Document Type: Article

References (22)

1. Altschul, S.F.; Gish, W.; Miller, W.; Myers, E.W.; Lipman, D.J. Basic local alignment search tool. J. Mol. Biol. 215:403-410;1990.
2. Astrup, T.; Müllertz, S. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys. 40:346-351; 1952.
3. Daoud, E.; Tu, A.T.; El-Asmar, M.F. Mechanism of the anticoagulant cerastase F-4, isolated from Cerastes cerastes (Egyptian sand viper) venom. Thromb. Res. 41:791-799;1986.
4. Datta, G.; Dong, A.; Witt, J.; Tu, A.T. Biochemical characterization of basilase, a fibrinolytic enzyme from Crotalus basiliscus basitiscus. Arch. Biochem. Biophys. 317:365-373;1995.
5. Hoppe, H.A. Marine algae and their products and constituents in pharmacy. In: Hoppe, H.A.; Leerving, T.; Tanaka, Y. (eds). Marine Algae in Pharmaceutical Science. Berlin: Walter de Gruyter; 1979:25-119.
6. Ito, K.; Hori K. Seaweed: Chemical composition and potential food uses. Food Rev. Int. 5:101-144;1989.
7. Kadokami, K.; Yoshida, N.; Mizusaki, K.; Noda, K.; Makisumi, S. Some properties of trypsin-like proteases extracted from the seaweed Codium fragile and their purification. Mar. Biol. 107: 513-517;1990.
8. Matsubara, K.; Sumi, H.; Hori, K. Platelet aggregation is inhibited by phycolectins. Experientia 52:540-543;1996.
9. Norris, J.N.; Fenical, W.H. Natural products chemistry: Uses in ecology and systematics. In: Litter, M.M.; Litter, D.S. (eds). Handbook of Phycological Methods. Ecological Field Methods: Macroalgae. New York: Cambridge University Press; 1985:123-145.
10. Ouyang, C.; Huang, T. Purification and characterization of the fibrinolytic principle of Agkistrodon acutus venom. Biochim. Biophys. Acta 439:146-153;1976.
11. Ouyang, C.; Huang, T. The properties of the purified fibrinolytic principle from Agldstrodon acutus snake venom. Toxicon 15:161-167;1977.
12. Ouyang, C.; Hwang, L.; Huang, T. α-Fibrinogenase from Agistrodon rhodostoma (Malayan pit viper) snake venom. Toxicon 21:25-33;1983.
13. Ouyang, C.; Teng, C. Fibrinogenolytic enzymes of Trimeresums mucrosquamatus venom. Biochim. Biophys. Acta 420: 298-308;1976.
14. Retzios, A.D.; Markland, F.S., Jr. A direct-acting fibrinolytic enzyme from the venom of Agkistrodon contortrix contortrix: Effects on various components of the human blood coagulation and fibrinolysis systems. Thromb. Res. 52:541-552;1988.
15. Schägger, H.; Jagow, G.V. Tricine-sodium dodecyl sulfatepolyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166: 368-379;1987.
16. Shimonaka, M.; Hagiwara, H.; Kojima, S.; Inada, Y. Successive study on the production of plasminogen activator in cultured endothelial cells by phytosterol. Thromb. Res. 36:217-222;1984.
17. Shiomi, K.; Hori, K. Hemagglutinins from algae. In: Akatsuka, I., (ed). Introduction to Applied Phychology. The Hague, The Netherlands: SPB Academic Publishing bV; 1990:93-108.
18. Siigur, E.; Mähar, A.; Siigur, J. β-Fibrinogenase from the venom of Vipera lebetina. Toxicon 29:107-118;1991.
19. Siigur, E.; Siigur, J. Purification and characterization of lebetase, a fibrinolytic enzyme from Vipera lebetina (snake) venom. Biochim. Biophys. Acta 1074:223-229;1991.
20. Sumi, H.; Nakajima, N.; Mihara, H. Fibrinolysis relating substances in marine creatures. Comp. Biochem. Physiol. 102B: 163-167;1992.
21. Utaisincharoen, P.; Mackessy, S.P.; Miller, R.A.; Tu, A.T. Complete primary structure and biochemical properties of gilatoxin, a serine protease with kallikrein-like and angiotensindegrading activities. J. Biol. Chem. 268:21975-21983;1993.
22. Willis, T.D.; Tu, A.T. Purification and biochemical characterization of atrozase, a nonhemorrhagic fibrinolytic protease from western diamondback rattlesnake venom. Biochemistry 27:4769-4777;1988.