A rapid method for combined laser scanning confocal microscopic and electron microscopic visualization of biocytin or Neurobiotin-labeled neurons

Journal of Histochemistry and Cytochemistry

Volumn 46, Issue 2, 1998, Pages 263-273

  Sun, Xue J ^a   Tolbert, Leslie P ^b   Hildebrand, John G ^b   Meinertzhagen, Ian A ^a  

^a DALHOUSIE UNIVERSITY   (Canada)

^b UNIVERSITY OF ARIZONA   (United States)

Author keywords

Biocytin; Confocal microscopy; Manduca sexta; Musca domestica; Neurobiotin; Olfaction; Reconstruction; Synapse; Three dimensional; Ultrastructure

Indexed keywords

BIOCYTIN;

ANIMAL CELL; ARTICLE; CELLULAR DISTRIBUTION; CONFOCAL LASER MICROSCOPY; ELECTRON MICROSCOPY; HOUSE FLY; IMAGE QUALITY; IMMUNOCYTOCHEMISTRY; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN LOCALIZATION; THREE DIMENSIONAL IMAGING;

ANIMALIA; MANDUCA SEXTA; MUSCA DOMESTICA;

EID: 0031911560     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215549804600216     Document Type: Article

References (43)

1. Bell RA, Joachim FA (1976) Techniques for rearing laboratory colonies of tobacco hornworms and pink bollworms. Ann Entomol Soc Am 69:365-373
2. Chen H, Swedlow JR, Grote M, Sedat JW, Agard DA (1995) The collection, processing, and display of digital three-dimensional images of biological specimens. In Pawley JB, ed. Handbook of Biological Confocal Microscopy. 2nd ed. New York, Plenum Press, 197-210
3. DeFelipe J, Fairén A (1993) A simple and reliable method for correlative light and electron microscopic studies. J Histochem Cytochem 41:769-772
4. Deitch JS, Smith KL, Lee CL, Swann JW, Turner JN (1990a) Confocal scanning laser microscope images of hippocampal neurons intracellular labeled with biocytin. J Neurosci Methods 33:61-76
5. Deitch JS, Smith KL, Swann JW, Turner JN (1990b) Parameters affecting imaging of the horseradish-peroxidase-diaminobenzidine reaction product in the confocal scanning microscope. J Microsc 160:265-278
6. Deitch JS, Smith KL, Swann JW, Turner JN (1991) Ultrastructural investigation of neurons identified and localized using the confocal scanning laser microscope. J Electron Microsc Tech 18:82-90
7. Fröhlich A, Meinertzhagen IA (1982) Synaptogenesis in the first optic neuropil of the fly's visual system. J Neurocytol 11:159-180
8. Hama K, Arii T, Kosaka T (1994) Three-dimensional organization of neuronal and glial processes: high voltage electron microscopy. Microsc Res Tech 29:357-367
9. Harris KM, Stevens JK (1988) Dendritic spines of rat cerebellar Purkinje cells: serial electron microscopy with reference to their biophysical characteristics. J Neurosci 8:4455-4469
10. Hill SJ, Oliver DL (1993) Visualization of neurons filled with biotinylated-Lucifer Yellow following identification of efferent connectivity with retrograde transport. J Neurosci Methods 46:59-68
11. Horikawa K, Armstrong WK (1988) A versatile means of intracellular labeling: injection of biocytin and its detection with avidin conjugates. J Neurosci Methods 25:1-11
12. Kawaguchi Y, Kubota Y (1996) Physiological and morphological identification of somatostatin/vasoactive intestinal polypeptide-containing cells among GABAergic cell subtypes in rat frontal cortex. J Neurosci 16:2701-2715
13. King MA, Louis PM, Hunter BE, Walker DW (1989) Biocytin: a versatile anterograde neuroanatomical tract tracing alternative Brain Res 497:361-367
14. Kita H, Armstrong W (1991) A biotin-containing compound N-(2-aminoethyl) biotinamide for intracellular labeling and neuronal tracing studies: comparison with biocytin. J Neurosci Methods 37:141-150
15. Kloppenburg P, Hildebrand JG (1995) Neuromodulation by 5-hydroxytryptamine in the antennal lobe of the sphinx moth Manduca sexta. J Exp Biol 198:603-611
16. Macagno ER, LoPresti V, Levinthal C (1973) Structure and development of neuronal connections in isogenic organisms: variations and similarities in the optic system of Daphnia magna. Proc Natl Acad Sci USA 70:57-61
17. Malun D (1991) Inventory and distribution of synapses of identified uniglomerular projection neurons in the antennal lobe of Periplaneta americana. J Comp Neurol 305:348-360
18. Maranto AR (1982) Neuronal mapping: a photooxidation reaction makes Lucifer Yellow useful for electron microscopy. Science 217:953-955
19. Martone ME, Zhang Y, Simpliciano VM, Carragher BO, Ellisman MH (1993) Three-dimensional visualization of the smooth endoplasmic reticulum in Purkinje cell dendrites. J Neurosci 13:4636-4646
20. Matsumoto SG, Hildebrand JG (1981) Olfactory mechanisms in the moth Manduca sexta: response characteristics and morphology of central neurons in the antennal lobes. Proc R Soc Lond [B] 213:249-277
21. Mercer AR, Hayashi JH, Hildebrand JG (1995) Modulatory effects of 5-hydroxytryptamine on voltage-activated currents in cultured antennal lobe neurones of the sphinx moth Manduca sexta. J Exp Biol 198:613-627
22. Mercer AR, Kloppenburg P, Hildebrand JG (1996) Serotonin-induced changes in the excitability of cultured antennal-lobe neurons of the sphinx moth Manduca sexta. J Comp Physiol [A] 178:21-31
23. Mesce KA, Klukas KA, Brelje TC (1993) Improvements for the anatomical characterization of insect neurons in whole mount: the use of cyanine-derived fluorophores and laser scanning confocal microscopy. Cell Tissue Res 271:381-397
24. Muller KJ, McMahan UJ (1976) The shape of sensory and motor neurons and the distribution of their synapses in ganglia of the leech: a study using intracellular injection of horseradish peroxidase. Proc R Soc Lond [B] 194:481-499
25. Nässel DR (1987) Serotonin and serotonin-immunoreactive neurons in the nervous system of insects. Prog Neurobiol 30:1-85
26. Pichon Y, Sattelle DB, Lane NJ (1972) Conduction processes in the nerve cord of the moth Manduca sexta in relation to its ultrastructure and haemolymph ionic composition. J Exp Biol 56:717-734
27. Pyza E, Meinertzhagen IA (1996) Neurotransmitters regulate rhythmic size changes amongst cells in the fly's optic lobe. J Comp Physiol 178:33-45
28. Rall W, Burke RE, Holmes WR, Jack JJ, Redman SJ, Segev I (1992) Matching dendritic neuron models to experimental data. Physiol Rev 72:S159-186
29. Rowell CH (1989) The taxonomy of invertebrate neurons: a plea for a new field. Trends Neurosci 12:169-174
30. Royer SM, Kinnamon JC (1994) Application of serial sectioning and three-dimensional reconstruction to the study of taste bud ultrastructure and organization. Microsc Res Tech 29:381-407
31. Sanes JR, Hildebrand JG (1976) Acetylcholine and its metabolic enzymes in developing antennae of the moth, Manduca sexta. Dev Biol 52:105-120
32. Soto GE, Young SJ, Ellisman MH (1994) Serial section electron tomography: a method for three-dimensional reconstruction of large structures. Neuroimage 1:230-243
33. Stevens JK, Davis TL, Friedman N, Sterling P (1980) A systematic approach to reconstructing microcircuitry by electron microscopy of serial sections. Brain Res 2:265-293
34. Stewart WW (1981) Lucifer dyes-highly fluorescent dyes for biological tracing. Nature 292:17-21
35. Sun XJ, Tolbert LP, Hildebrand JG (1993) Ramification pattern and ultrastructural characteristics of the serotonin-immunoreactive neuron in the antennal lobe of the moth Manduca sexta: a laser scanning confocal and electron microscopic study. J Comp Neurol 338:1-16
36. Sun XJ, Tolbert LP, Hildebrand JG (1995) Using laser scanning confocal microscopy as a guide for electron microscopic study - a simple method for correlation of light and electron microscopy. J Histochem Cytochem 43:329-335
37. Sun XJ, Tolbert LP, Hildebrand JG (1997) Synaptic distribution and connections of the uniglomerular projection neurons of the antennal lobe of Manduca sexta. J Comp Neurol 379:2-20
38. Sun XJ, Tolbert LP, Hildebrand JG, Meinertzhagen IA (1996) A rapid method for combined confocal microscopic and EM visualization of biocytin- or Neurobionn-labeled neurons. Soc Neurosci Abstr 22:582
39. Taghert PH, Bastiani MJ, Ho RK, Goodman CS (1982) Guidance of pioneer growth cones: filopodial contacts and coupling revealed with an antibody to Lucifer Yellow. Dev Biol 94:391-399
40. Tasker JG, Hoffman NW, Dudek FE (1991) Comparison of three intracellular markers for combined electrophysiological, morphological and immunohistochemical analysis. J Neurosci Methods 38:129-143
41. Tolbert LP, Sun XJ, Hildebrand JG (1996) Combined laser scanning confocal microscopy and electron microscopy in studies of the insect nervous system. J Neurosci Methods 69:25-32
42. Turner JN, Szarowski DH, Smith KL, Marko M, Leith A, Swann JW (1991) Confocal microscopy and three-dimensional reconstruction of electrophysiological identified neurons in thick brain slices. J Electron Microsc Tech 18:11-23
43. Ware RW, LoPresti V (1975) Three-dimensional reconstruction from serial sections. Int Rev Cytol 40:325-440