A silver impregnation utilizing only reagent-grade chemicals for visualization of peripheral axons and fibroblasts

Journal of Microscopy

Volumn 189, Issue 2, 1998, Pages 154-162

  Novotny, G E K ^a   Mertens, I ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

Connective tissue; Fibroblasts; Nerves; Silver impregnation

Indexed keywords

CELL CULTURE; COLLAGEN; IMPREGNATION; MUSCULOSKELETAL SYSTEM; SILVER; TISSUE; VISUALIZATION;

ANALYTICAL GRADES; AUTONOMICS; CELL PROCESS; CONNECTIVE TISSUES; NERVE; REAGENT GRADES; REAGENT WATER; SILVER IMPREGNATION; THREE-DIMENSIONAL ANALYSIS; TISSUE COMPONENTS;

FIBROBLASTS;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONFOCAL LASER MICROSCOPY; FIBROBLAST; HUMAN; HUMAN CELL; HUMAN TISSUE; INNERVATION; NERVE ENDING; NERVE FIBER; NONHUMAN; PERIPHERAL NERVE; PRIORITY JOURNAL; RAT; SILVER IMPREGNATION; THREE DIMENSIONAL IMAGING; TISSUE FIXATION;

EID: 0031911041     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1998.00288.x     Document Type: Article

References (12)

1. Andres, K.H. (1966) Über die Feinstruktur der Rezeptoren an Sinushaaren. Z. Zellforsch. 75, 339-365.
2. Autilio-Gambetti, L, Crane, R. & Gambetti, P. (1986) Binding of Bodian's silver and monoclonal antibodies to defined regions of human neurofilament subunits: Bodian's silver reacts with a highly charged unique domain of neurofilaments. J. Neurochem. 46, 366-370.
3. Bayreuther, K., Rodemann, H.P., Francz, P.I. & Maier, K. (1988) Differentiation of fibroblast stem cells. J. Cell Sci. (Suppl.), 10, 115-130.
4. Boyde, A. (1985) Stereoscopic images in confocal (tandem scanning) microscopy. Science 230, 1270-1272.
5. Gabe, (1976) Histological Techniques. Springer-Verlag, New York.
6. Glees, P. (1946) Terminal degeneration within the central nervous system as studied by a new silver method. J. Neuropath. Exp. Neurol. 5, 54-59.
7. Novotny, G.E.K. & Gommert-Novotny, E. (1988) Silver impregnation of peripheral and central axons. Stain Technol. 63, 1-14.
8. Novotny, G.E.K. & Gommert-Novotny, E. (1990) A simple procedure for demonstrating the overall morphology of fibroblasts in routine histological preparations of adult tissues, using silver impregnation. J. Microsc. 159, 99-107.
9. Palmgren, A. (1960) Specific silver staining of nerve fibres. I. Technique for vertebrates. Acta Zool. (Stockholm), 41, 239-265.
10. Saalbach, A., Anderegg, U., Bruns, M., Schnabel, E., Herrmann, K. & Haustein, U.F. (1996) Novel fibroblast-specific monoclonal antibodies: properties and specificities. J. Invest. Dermatol. 106, 1314-1319.
11. Silverman, R.T., Munger, B.L. & Halata, Z. (1986) The sensory innervation of the rat rhinarium. Anat. Record. 214, 210-225.
12. Wolman, M. (1955) Studies of the silver impregnation of nervous tissue elements. II. The nature of the compounds responsible for the impregnation of axons: pactical considerations. Q. J. Microsc. Sci. 96, 337-341.