Cloning and functional expression of the human GlcNAc-1-P transferase, the enzyme for the committed step of the dolichol cycle, by heterologous complementation in Saccharomyces cerevisiae

Glycobiology

Volumn 8, Issue 1, 1998, Pages 77-85

  Eckert, Volker ^a   Blank, Michaela ^a   Mazhari Tabrizi, Ramin ^a   Mumberg, Dominik ^a,b   Funk, Martin ^a,c   Schwarz, Ralph T ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

^b UNIVERSITY OF CHICAGO   (United States)

^c MediGene AG   (Germany)

Author keywords

Complementation cloning; Conditional lethality; Dolichol cycle; Glycosyltransferase; Heterologous expression

Indexed keywords

GLYCOSYLTRANSFERASE;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; DNA LIBRARY; ENZYME ACTIVITY; GENE CONSTRUCT; GENETIC COMPLEMENTATION; HUMAN; HUMAN TISSUE; LEISHMANIA; LUNG FIBROBLAST; MOLECULAR CLONING; PHYLOGENY; PRIORITY JOURNAL; RECOMBINANT PLASMID; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE; SEQUENCE HOMOLOGY; TECHNIQUE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; DNA, COMPLEMENTARY; DOLICHOL; DRUG RESISTANCE, MICROBIAL; GENE EXPRESSION; GENETIC COMPLEMENTATION TEST; HUMANS; MOLECULAR SEQUENCE DATA; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; TRANSFERASES (OTHER SUBSTITUTED PHOSPHATE GROUPS); TUNICAMYCIN;

LEISHMANIA AMAZONENSIS; MAMMALIA; PROTOZOA; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE;

EID: 0031910552     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/8.1.77     Document Type: Article

References (55)

1. Baudin, A., Ozier-Kalogeropoulos, O., Denouel, A., Lacroute, F. and Cullin, C. (1993) A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucleic Acids Res., 21, 3329-3330.
2. Boeke, J.D., Truehyrt, J., Natsoulis, G. and Fink, G. (1987) 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol., 154, 164-175.
3. Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-cholroform extraction. Anal. Biochem., 162, 156-159.
4. Datta, A. and Lehrman, M.A. (1993) Both potential dolichol recognition sequences of hamster GlcNAc-1 -phosphate transferase are necessary for normal enzyme function. J. Biol. Chem., 268, 12663-12668.
5. Eckert, V., Gerold, P. and Schwarz, R.T. (1997) GPI-anchors: structure and functions. In Gabius, H.-J. and Gabius, S. (eds.) Glycosciences. Chapman and Hall, Weinheim, Germany, pp. 223-243.
6. Elbein, A.D. (1987) Inhibitors of the biosynthesis and processing of N-linked oligosaccharide chains. Annu. Rev. Biochem., 56, 497-534.
7. Elledge, J.S. and Spottswood, M.R. (1991) A new human p34 protein kinase, CDK2, identified by complementation of a cdc28 mutation in Saccharomyces cerevisiae, is a homolog of Xenopus EG1. EMBO J., 19, 2653-2659.
8. Fiedler, K. and Simons, K. (1995) The role of N-glycans in the secretory pathway. Cell, 81, 309-312.
9. Gerold, P., Dieckmann-Schuppert, A. and Schwarz, R.T. (1994) Glycosylphosphatidylinositols synthesized by asexuell erythrocytic stages of the malaria parasite Plasmodium falciparum. J. Biol. Chem., 269, 2597-2606.
10. Hammond, C. Braakman, I. and Helenius, A. (1994) Role of N-linked oligosaccharide recognition, glucose trimming, and calnexin in glycoprotein folding and quality control. Proc. Natl. Acad. Sci. USA, 91, 913-917.
11. Hammond, C. and Helenius, A. (1995) Quality control in the secretory pathway. Curr. Opinion Cell. Biol., 7, 523-529.
12. Hartog, K.O. and Bishop, B. (1987) Genomic sequence coding for tunicamycin resistance in yeast. Nucleic Acids Res., 15, 3627.
13. Hayflick, L. (1965) The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res., 37, 614-636.
14. Hoffman, C.S. and Winston, F. (1987) A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformantion of Escherichia coli. Gene, 57, 267-272.
15. Huffaker, T.C. and Robbins, P.W. (1983) Yeast mutants deficient in protein glycosylation. Proc. Natl. Acad. Sci. USA., 80, 7466-7470.
16. Ioffe, E. and Stanley, P. (1994) Mice lacking N-acetylglucosaminyltransferase I activity die at mid-gestation, revealing an essential role for complex or hybrid N-linked carbohydrates. Proc. Natl. Acad. Sci. USA, 91, 728-732.
17. Ito, H., Fukada, Y., Murata, K. and Kimura, (1983) Transformation of intact yeast cells treated with alkali cations. J. Bacteriol., 153, 163-168.
18. Johnston, M. and Davis, R.W. (1984) Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Mol. Cell. Biol., 4, 1440-1448.
19. Kaushal, G.P. and Elbein, A.D. (1994) Glycosidase inhibitors in study of glycoconjugates. Methods Enzymol., 239, 316-329.
20. Kornfeld, R. and Kornfeld, S. (1985) Assembly of asparagine-linked oligosaccharides. Annu. Rev. Biochem., 54, 631-664.
21. Kukuruzinska, M.A. and Robbins, P.W. (1987) Protein glycosylation in yeast: tanscript heterogeneity of the ALG7 gene. Proc. Natl. Acad. Sci. USA, 84, 2145-2149.
22. Lehrman, M.A. (1991) Biosynthesis of N-aetylglucosamine-P-P-dolichol, the committed step of asparagine-linked oligosaccharide assembly. Glycobiology, 1, 553-562.
23. Lehrman, M.A., Zhu, X. and Khounlo, S. (1988) Amplification and molecular cloning of the hamster tunicamycin-sensitive N-acetylglucosamine-1- phosphate transferase gene. J. Biol. Chem., 263, 19796-19803.
24. Liu, X. and Chang, K.-P. (1992) The 63-kilobase circular amplicon of tunicamycin-resistant Leishmania amazonensis contains a functional N-acetylglucosamine-1-phosphate transferase gene that can be used as a dominant selectable marker in transfection. Mol. Cell. Biol., 12, 4112-4122.
25. Lorenz, M.C., Muir, R.S., Lim, E., McElver, J., Weber, S.C. and Heitman, J. (1995) Gene disruption with PCR products in Saccharomyces cerevisiae. Gene, 158, 113-117.
26. Manivasakam, P., Weber, S.C., McElver, J. and Schistel, R.H. (1995) Microhomology mediated PCR targeting in Saccharomyces cerevisiae. Nucleic Acids Res., 23, 2799-2800.
27. Marth, J.D. (1994) Will the transgenic mouse serve as a rosetta stone to glycoconjugate function? Glycoconjugate J., 11, 3-8.
28. Mazhari-Tabrizi, R., Eckert, V., Blank, M., Muller, R., Mumberg, D., Funk, M. and Schwarz, R.T. (1996) Cloning and functional expression of glycosyltransferases from parasitic protozoa by heterologous complementation in yeast: the Dol-P-Man synthase from Trypanosoma brucei brucei. Biochem. J., 316, 853-858.
29. McDowell, W. and Schwarz, R.T. (1988) Lipid-mediated protein glycosylation: assembly of lipid linked oligosaccharides and post-translational oligosaccharide trimming. In Brodbeck, U. and Bordier, C. (eds.), Posttranslational Modifications of Proteins by Lipids. C. Springer, Berlin, pp. 99-118.
30. Metzler, M., Gertz, A., Sarkar, M., Schachter, H., Schrader, J.W. and Marth, J.D. (1994) Complex asparagine-linked oligosaccharides are required for morphogenic events during post-implantation development. EMBO J., 13, 2056-2065.
31. Minet, M., Dufour, M.-E. and Lacroute, F. (1992) Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thalania cDNAs. Plant J., 2, 417-422.
32. Mumberg, D., Müller, R. and Funk, M. (1994) Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res., 22, 5767-5768.
33. Needleman, S.B. and Wunsch, C.D. (1970) A general method applicable to the search for similarities in the amino acid sequence of two proteins. J. Mol. Biol., 48, 443-453.
34. Rajput, B., Ma, J., Munipappa, N., Schantz, L., Naylor, S.L., Lalley, P.A. and Vijay, I.K. (1992) Mouse UDP-GlcNAc: dolichyl-phosphate N-acetylglucosaminephosphotransferase. Molecular cloning of the cDNA, generation of anti-peptide antibodies and chromosomal location. Biochem. J., 285, 985-992.
35. Rajput, B., Ma, J. and Vijay, I.K. (1994) Structure and organization of mouse GlcNAc-1-phosphate transferase gene. J. Biol. Chem., 269, 9590-9597.
36. Rine, J., Hansen, W., Hardeman, E. and Davis, R.W. (1983) Targeted selection of recombinant clones through gene dosage effects. Proc. Natl. Acad. Sci. USA, 80, 6750-6754.
37. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
38. Schachter, H. (1995) Glycosyltransferases involved in the synthesis of N-glycan antennae. In Montreul, J., Vliegenhthart, J.F.G. and Schachter, H. (eds.), Glycoproteins 29a. Elsevier, Amsterdam, pp. 153-199.
39. Schönbächler, M., Horvath, A., Fassler, J. and Riezman, H. (1995) The yeast spt14 gene is homologous to the human PIG-A gene and is required for GPI anchor synthesis. EMBO J., 14, 1637-1645.
40. Scocca, J.R. and Krag, S.S. (1990) Sequence of a cDNA that specifies the uridine diphosphate N-acetyl-d-glucosamine:dolichol phosphate N-acetylglucosamine-1-phosphate transferase from Chinese hamster ovary cells. J. Biol. Chem., 265, 20621-20626.
41. Scocca, J.R., Zou, J. and Krag, S.S. (1995) Genomic organization and expression of hamster UDP-N-acetylglucosamine: dolichyl phosphate N-acetylglucosaminyl phosphoryl transferase. Glycobiology, 5, 129-136.
42. Schutzbach, J.S., Zimmerman, J.W. and Forsee, T.W. (1993) The purification and characterisation of recombinant yeast dolichyl-phosphate-mannose synthase. J. Biol Chem., 268, 24190-24196.
43. Schwarz, R.T. and Datema, R. (1980) Inhibitors of protein glycosylation. Trends Biochem. Sci., 5, 65-67.
44. Schwarz, R.T. and Datema, R. (1982) The lipid pathway of protein glycosylation and its inhibitors: the biological significance of protein-bound carbohydrates. Adv. Carbohydr. Chem. Biochem., 40, 287-379.
45. Sharma, C.B., Babczinski, P., Uhle, and Tanner, W. (1974) The role of dolicolmonophosphate in glycoprotein biosynthesis in Saccharomyces cerevisiae. Eur. J. Biochem., 46, 35-41.
46. Sikorski, R.S. and Hieter, P. (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics, 122, 19-27.
47. Sousa, M.C., Ferrero-Garcia, M.A. and Parodi, A.J. (1992) Recognition of the oligosaccharide and protein moieties of glycoproteins by the UDP-Glc:glycoprotein glucosyltransferase. Biochemistry, 31, 95-107.
48. Stanley, p., and Ioffe, E. (1995) Glycosyltransferase mutants: key to new insights in glycobiology. FASEB J., 9, 1436-1444.
49. Takeda, J. and Kinoshita, T. (1995) GPI-anchor biosynthesis. Trends Biochem. Sci., 20, 367-371.
50. Varki, A. (1993) Biological roles of oligosaccharides: all of the theories are correct. Glycobiology, 3, 97-130.
51. Von Heijne, G. (1983) Patterns of amino acids near signal-sequence cleavage sites. Eur. J. Biochem., 133, 17-21.
52. Wach, A., Brachat, A., Pöhlmann, R. and Philippsen, P. (1994) New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast, 10, 1793-1808.
53. Zara, J. and Lehrman, M.A. (1994) Role of the carboxyl terminus in stable expression of hamster UDP-GlcNAc:dolichol-PGlcNAc-1-P transferase. J. Biol. Chem., 269, 19108-19115.
54. Zhu, X. and Lehrman, M.A. (1990) Cloning, sequence and expression of a cDNA encoding hamster UDP-GlcNAc:dolichol phosphate N-acetylglucosamine-1-phosphate transferase. J. Biol. Chem., 265, 14250-14255.
55. Zou, J., Scocca, J.R. and Krag, S.S. (1995) Asparagine-linked glycosylation in Schizosaccharomyces pombe: functional conservation of the first step in oligosaccharide-lipid assembly. Arch. Biochem. Biophys., 317, 487-496.