The NS1 protein of human respiratory syncytial virus is a potent inhibitor of minigenome transcription and RNA replication

Journal of Virology

Volumn 72, Issue 2, 1998, Pages 1452-1461

  Atreya, Prabha L ^a,c   Peeples, Mark E ^a,b   Collins, Peter L ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b RUSH UNIVERSITY MEDICAL CENTER   (United States)

^c CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELONGATION FACTOR; VIRUS PROTEIN;

ARTICLE; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; REPLICON; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; RNA REPLICATION; RNA SYNTHESIS; TRANSCRIPTION REGULATION; VIRUS CAPSID; VIRUS GENOME;

EID: 0031907721     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.72.2.1452-1461.1998     Document Type: Article

References (32)

1. Alansari, H., and L. N. D. Potgeiter. 1994. Nucleotide and predicted amino acid sequence analysis of the ovine respiratory syncytial virus nonstructural 1C and 1B genes and the small hydrophobic protein gene. J. Gen. Virol. 75:401-404.
2. Baker, S. C., and S. A. Moyer. 1988. Encapsidation of Sendai virus genome RNAs by purified NP protein during in vitro replication. J. Virol. 62:834-838.
3. Cadd, T., D. Garcin, C. Tapparel, M. Itoh, M. Homma, L. Roux, J. Curran, and D. Kolakofsky. 1996. The Sendai paramyxovirus accessory C proteins inhibit viral genome amplification in a promoter-specific fashion. J. Virol. 70:5067-5074.
4. Chomczynski, P. 1992. One hour downward alkaline capillary transfer for blotting of DNA and RNA. Anal. Biochem. 20:134-139.
5. Collins, P. L., and G. W. Wertz. 1983. cDNA cloning and transcriptional mapping of nine polyadenylated RNAs encoded by the genome of human respiratory syncytial virus. Proc. Natl. Acad. Sci. USA 80:3208-3212.
6. Collins, P. L., and G. W. Wertz. 1985. Nucleotide sequences of the 1B and 1C nonstructural protein mRNAs of human respiratory syncytial virus. Virology 143:442-451.
7. Collins, P. L. 1991. The molecular biology of human respiratory syncytial virus (RSV) of genus Pneumovirus, p. 103-162. In D. W. Kingsbury (ed.), The paramyxoviruses. Plenum Publishing Corp., New York, N.Y.
8. Collins, P. L., M. G. Hill, E. Camargo, H. Grosfeld, R. M. Chanock, and B. R. Murphy. 1995. Production of infectious human respiratory syncytial virus from cloned cDNA confirms an essential role for the M2 (ORF1) transcription elongation factor in gene expression and provides a new capability for vaccine development. Proc. Natl. Acad. Sci. USA 92:11563-11567.
9. Collins, P. L., M. G. Hill, J. Cristina, and H. Grosfeld. 1996. Transcription elongation factor of respiratory syncytial virus, a nonsegmented negative-strand RNA virus. Proc. Natl. Acad. Sci. USA 93:81-85.
10. Collins, P. L., K. McIntosh, and R. M. Chanock. 1996. Respiratory syncytial virus, p. 1313-1352. In B. N. Fields, D. M. Knipe, and P. M. Howley (ed.), Virology, 3rd ed. Raven Press, New York, N.Y.
11. Curran, J., J.-B. Marq, and D. Kolakofsky. 1992. The Sendai virus nonstructural C proteins specifically inhibit viral mRNA synthesis. Virology 189:647-656.
12. Durbin, A. P., J. W. Siew, B. R. Murphy, and P. L. Collins. 1997. Minimum requirements for transcription and RNA replication of a minigenome of human parainfluenza virus type 3. Virology 234:74-83.
13. Elroy-Stein, O, T. R. Fuerst, and B. Moss. 1989. Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5′ sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid system. Proc. Natl. Acad. Sci. USA 86:6126-6130.
14. Fearns, R., M. E. Peeples, and P. L. Collins. 1997. Increased expression of the N protein of respiratory syncytial virus stimulates minigenome replication but does not alter the balance between the synthesis of mRNA and antigenome. Virology 236:188-201.
15. Fuerst, T. R., E. G. Niles, F. W. Studier, and B. Moss. 1986. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 83:8122-8126.
16. Gorman, C. M., L. F. Moffat, and B. H. Howard. 1982. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol. 2:1044-1051.
17. Grosfeld, H., M. G. Hill, and P. L. Collins. 1995. RNA replication by respiratory syncytial virus (RSV) is directed by the N, P, and L proteins: transcription also occurs under these conditions but required RSV superinfection for efficient synthesis of full-length mRNA. J. Virol. 69:5677-5686.
18. Horikami, S., S. Smallwood, and S. Moyer. 1996. The Sendai virus V protein interacts with the NP protein to regulate viral genome RNA replication. Virology 222:383-390.
19. Iverson, L. E., and J. K. Rose. 1981. Localized attenuation and discontinuous synthesis during vesicular stomatitis virus transcription. Cell 23:477-484.
20. Johnson, P. R., and P. L. Collins. 1989. The 1B (NS2), 1C (NS1) and N proteins of human respiratory syncytial virus (RSV) of antigenic subgroups A and B: sequence conservation and divergence within RSV genomic RNA. J. Gen. Virol. 70:1539-1547.
21. Kuo, L., H. Grosfeld, J. Cristina, M. G. Hill, and P. L. Collins. 1996. Effect of Mutations in the gene-start and gene-end sequence motifs on transcription of monocistronic and dicistronic minigenomes of respiratory syncytial virus. J. Virol. 70:6892-6901.
22. Kuo, L., R. Fearns, and P. L. Collins. 1996. The structurally diverse intergenic regions of respiratory syncytial virus do not modulate sequential transcription by a dicistronic minigenome. J. Virol. 70:6143-6150.
23. Kuo, L., R. Fearns, and P. L. Collins. 1997. Analysis of the gene start and gene end signals of human respiratory syncytial virus: quasi-templated initiation at position 1 of the encoded mRNA. J. Virol. 71:4944-4953.
24. Lamb, R. A., and D. Kolakofsky. 1996. Paramyxoviridae: the viruses and their replication, p. 1177-1204. In B. N. Fields, D. M. Knipe, and P. M. Howley (ed.), Virology, 3rd ed. Raven Press, New York, N.Y.
25. Patsey, M., and S. K. Samal. 1995. Nucleotide sequence analysis of the non-structural NS1 (1C) and NS2 (1B) protein genes of bovine respiratory syncytial virus. J. Gen. Virol. 76:193-197.
26. Peeples, M. E., R. Fearns, J. Cristina, and P. L. Collins. Unpublished data.
27. Peeples, M. E., R. Fearns, and P. L. Collins. Unpublished data.
28. Perotta, A. T., and M. D. Been. 1991. A pseudoknot-like structure required for efficient self-cleavage of hepatitis delta virus RNA. Nature 350:434-436.
29. Radecke, F., and M. A. Billeter. 1996. The nonstructural C protein is not essential for multiplication of Edmonston B strain measles virus in cultured cells. Virology 217:418-421.
30. Teng, M., A. Durbin, and M. Skiadopoulos. Unpublished data.
31. Towbin, H., R. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
32. Wagner, R. R., and J. K. Rose. 1996. Rhabdoviridae and their replication, p. 1121-1135. In B. N. Fields, D. M. Knipe, and P. M. Howley (ed.), Virology, 3rd ed. Raven Press, New York, N.Y.