In-vitro human spermatozoa nuclear decondensation assessed by flow cytometry

Molecular Human Reproduction

Volumn 4, Issue 2, 1998, Pages 133-137

  Samocha Bone, D ^a   Lewin, L M ^a   Weissenberg, R ^b   Madgar, Y ^b   Soffer, Y ^c   Shochat, L ^a   Golan, R ^a  

^a TEL AVIV UNIVERSITY   (Israel)

^b SHEBA MEDICAL CENTER   (Israel)

^c ASSAF HAROFEH MEDICAL CENTER   (Israel)

Author keywords

Acridine Orange; Chromatin decondensation; Flow cytometry; Human; Spermatozoa

Indexed keywords

ACRIDINE ORANGE; FORMALDEHYDE; HEPARIN; MERCAPTOETHANOL; POLYANION;

ANIMAL CELL; ARTICLE; CHROMATIN CONDENSATION; CONTROLLED STUDY; FERTILIZATION; FLOW CYTOMETRY; HAMSTER; HUMAN; HUMAN CELL; MALE; NONHUMAN; OOCYTE; PRIORITY JOURNAL; SPERMATOZOON;

ANIMALS; CELL NUCLEUS; CRICETINAE; FEMALE; FLOW CYTOMETRY; HUMANS; MALE; SPERM-OVUM INTERACTIONS; SPERMATOZOA;

EID: 0031907451     PISSN: 13609947     EISSN: None     Source Type: Journal    
DOI: 10.1093/molehr/4.2.133     Document Type: Article

References (33)

1. Auger, J. and Dadoune, J.-P. (1993) Nuclear status of human sperm cells by transmission electron microscopy and image cytometry: changes in nuclear shape and chromatin texture during spermatogenesis and epididymal transit. Biol. Reprod., 49, 166-175.
2. Banerjee, S. and Hulten, M.A.J. (1994) Sperm nuclear chromatin transformations in somatic cell-free extracts. Mol. Reprod. Dev. 37, 305-317.
3. Betzalel, M., Shalgi, R. and Moav, B. (1986) Protamine in the rat; its fate in vivo, and its degradation in vitro by egg homogenate. Gamete Res, 14, 293-306.
4. Brown, D.B. and Nagamani, M. (1992) Use of Xenopus laevis frog egg extract in diagnosing human male unexplained infertility. Yale J. Biol. Med., 65, 29-38.
5. Burkman, L.J, Coddington, C.C., Franken, D.R. et al. (1988) The hemizona assay (HZA): development of a diagnostic test for the binding of human spermatozoa to the human hemizona pellucida to predict fertilization potential. Fertil. Steril., 49, 688-697.
6. Cameron, L.A. and Poccia, D.L. (1994) In vitro development of the sea urchin male pronucleus. Dev. Biol., 162, 568-578.
7. Chan, P.J. and Tredway, D.R. (1992) Association of human sperm nuclear decondensation and in vitro penetration ability. Andrologia, 24, 77-81.
8. Chitale, A.R. and Rathaur, R.G. (1995) Nuclear decondensation of sperm head and failure at in vitro fertilization: an ultrastructural study. Hum. Reprod., 10, 594-598.
9. Evenson, D.P., Darzynkiewicz, Z., Melamed, M.R. (1980) Comparison of human and mouse sperm chromatin structure by flow cytometry. Chromasoma, 78, 225-238.
10. Evenson, D.P., Darzynkiewicz, Z., Jost, L. et al. (1986) Changes in accessibility of DNA to various fluorochromes during spermiogenesis. Cytometry, 7, 45-53.
11. Golan, R., Cooper, T.G., Oschry, Y. et al. (1996) Changes in chromatin condensation of human spermatozoa during epididymal transit as determined by flow cytometry. Hum. Reprod., 11, 1457-1462.
12. Golan, R., Shochat, L., Weissenberg, R. et al. (1997) Evaluation of chromatin condensation in human spermatozoa: a flow cytometric assay using acridine orange staining. Mol. Hum. Reprod., 3, 47-54.
13. Griveau, J.F., Charbonneau, M., Blanchard, Y. et al. (1992) Decondensation of human sperm nuclei and HP1 protamine degradation from normospermia and asthenospermia in Xenopus egg extracts. Archives Androl., 29 ,127-136.
14. Jager, S., Wijchman, J. and Kremer, J. (1990) Studies on the decondensation of human, mouse and bull sperm nuclei by heparin and other polyanions. J. Exp. Zool., 256, 315-322.
15. Katagiri, C. and Ohsumi, K. (1994) Remodeling of sperm chromatin induced in egg extracts of amphibians. Int. J. Dev. Biol., 38, 209-216.
16. Lassalle, B. and Testart, J. (1991) Sequential transformation of human sperm nucleus in human egg. J. Reprod. Fertil., 91, 393-402.
17. Leno, G.H., Mills, A.D., Philpott, A. and Laskey, R.A. (1996) Hyperphosphorylation of nucleoplasmin facilitates Xenopus sperm decondensation at fertilization. J. Biol. Chem., 271, 7253-7256.
18. Lipitz, S., Bartoov, B., Rajuan, C. et al. (1992) Sperm head ultramorphology and chromatin stability of males with unexplained infertility who fail to fertilize normal human ova in vitro. Andrologia, 24, 261-269.
19. Montag, M., Tbk, V., Liow, S.-L. et al. (1992) In vitro decondensation of mammalian sperm and subsequent formation of pronuclei-like structures for micromanipulation. Mol. Reprod. Devel., 33, 338-346.
20. Morcos, Y. and Swan, M.A. (1994) Reactivated movement of decondensed rat sperm models and a description of their ultrastructure. J. Exp. Zool., 270, 388-398.
21. Philpott, A., Leno, G.H. and Laskey R.A. (1991) Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin. Cell, 65, 569-578.
22. Philpott, A. and Leno, G.H. (1992) Nucleoplasmin remodels sperm chromatin in Xenopus egg extracts. Cell, 69, 759-767.
23. Perreault, S.D. (1992) Chromatin remodeling in mammalian zygotes. Mutat. Res., 296, 43-55.
24. Perreault, S.D., Wollf, R.A. and Zirkin, B.R. (1984) The role of disulfide bond reduction during mammalian sperm nuclear decondensation in vivo. Dev. Biol., 101, 160-167.
25. Perreault, S.D., Naish, S.J. and Zirkin, B.R. (1987) The timing of hamster sperm nuclear decondensation and male pronucleus formation is related to sperm nuclear disulfide bond content. Biol. Reprod. 36, 239-244.
26. Perreault, S.D., Barbee, R.R. and Slott, V.L. (1988) Importance of glutathione in the acquisition and maintenance of sperm nuclear decondensing activity in maturing hamster oocytes. Dev. Biol., 125, 181-186.
27. Reyes, R., Sanchez-Vazquez, M.L., Merchant-Larios, H. et al. (1996) Effect of heparin-reduced glutathione on hamster sperm DNA unpacking and nuclear swelling. Arch. Androl., 37, 33-45.
28. Sakkas, D., Urner, F., Bianchi, P.G. et al. (1996) Sperm chromatin anomalies can influence decondensation after intracytoplasmic sperm injection. Hum. Reprod., 11, 837-843.
29. Soffer, Y., Golan, A., Herman, A. et al. (1992) Prediction of in vivo fertilization outcome by sperm penetration assay with TEST-yolk buffer preincubation. Fertil. Steril., 58, 556-562.
30. World Health Organization (1987) Laboratory Manual for the Examination of Human Semen and Cervical-Mucus Interaction. Cambridge University Press, Cambridge, U.K.
31. Yossefi, S., Oschry, Y. and Lewin, L.M. (1994) Chromatin condensation in hamster sperm: a flow cytometric investigation. Mol. Reprod. Dev., 37, 93-98.
32. Zirkin, B.R., Soucek, D.A., Chang, T.S.K. and Perreault, S.D. (1985) In vitro and in vivo studies of mammalian sperm nuclear decondensation. Gamete Res., 11, 349-365.
33. Zucker, R.M., Perreault, S.D. and Elstein, K.H. (1992) Utility of light scatter in the morphological analysis of sperm. Cytometry, 13, 39-47.