The application of a biotin-anti-biotin gold technique providing a significant signal intensification in electron microscopic immunocytochemistry: A comparison with the ultrasmall immunogold silver staining procedure

Histochemistry and Cell Biology

Volumn 109, Issue 2, 1998, Pages 119-125

  Muller Hocker J ^a   Schafer S ^a   Sendelhofert, A ^a   Weis, S ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTIN; CYTOCHROME C OXIDASE; GLUCAGON; GOLD;

ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; BIOTINYLATION; COLLOID; CONTROLLED STUDY; ELECTRON MICROSCOPY; HEART MITOCHONDRION; HEART MUSCLE; HUMAN; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; IMMUNOGOLD STAINING; IMMUNOHISTOCHEMISTRY; INTERMETHOD COMPARISON; NONHUMAN; PANCREAS ISLET; PRIORITY JOURNAL; RAT; REDUCTION; SILVER STAINING;

ANIMALS; BIOTIN; ELECTRON TRANSPORT COMPLEX IV; EVALUATION STUDIES; GLUCAGON; HUMANS; IMMUNOHISTOCHEMISTRY; ISLETS OF LANGERHANS; MICROSCOPY, IMMUNOELECTRON; MITOCHONDRIA, HEART; MYOCARDIUM; RATS; SILVER; STAINING AND LABELING;

ANIMALIA;

EID: 0031907308     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004180050209     Document Type: Article

References (48)

1. Adams JC (1992) Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains. J Histochem Cytochem 40:1457-1463
2. Bendayan M (1984) Protein A-gold electron microscopic immunocytochemistry: methods, applications and limitations. J Electron Microsc Tech 1:243-270
3. Berghorn KA, Bonnett JH, Hoffman GE (1994) sFos immunoreactivity is enhanced with biotin amplification. J Histochem Cytochem 42:1635-1642
4. Brändli AW, Parton RG, Simons K (1990) Transcytosis in MDCK cells: identification of glycoproteins transported bidirectionally between both plasma membrane domains. J Cell Biol 111: 2909-2922
5. Chamlian A, Benkoel L, Ikoli JF, Brisse J, Jacob T (1993) Nuclear immunostaining of hepatitis C infected hepatocytes with monoclonal antibodies to C 100-3 nonstructural protein. Comparison of immunogold silver staining with other immunocytochemical methods. Cell Mol Biol Res 39:243-251
6. Childs GV, Unabia G (1982) Application of rapid avidin-biotinperoxidase complex (ABC) technique to the localization of pituitary hormones at the electron microscopic level. J Histochem Cytochem 30:1320-1324
7. Dankner WM, Spector SA (1989) Applications of immunogold-silver enhancement: testing of monoclonal antibodies and detection of human cytomegalovirus in histologic specimens. Am J Anat 185:310-313
8. Danscher G (1981) Localization of gold in biological tissue. Histochemistry 71:81-88
9. Danscher G, Rytter Nörgaard JO (1983) Light microscopic visualization of colloidal gold on resin-embedded tissue. J Histochem Cytochem 31:1994-1398
10. Gee B, Warhol MJ, Roth J (1991) Use of an anti-horseradish peroxidase antibody-gold complex in the ABC-technique. J Histochem Cytochem 39:863-869
11. Ghitescu L, Bendayan M (1990) Immunolabelling efficiency of protein A-gold complexes. J Histochem Cytochem 38:1523-1530
12. Giffin BF, Gao K, Morris RE, Cardell RR (1993) Enhancement of antigenic site detection with gold labeled secondary and tertiary antibodies using the immunogold-silver staining method. Biotech Histochem 68:309-315
13. Goggi G, Dell'Orto P, Viale G (1986) Avidin-biotin methods. In: Polak JM, Van Noorden S (eds) Immunocytochemistry, 2nd edn. John Wright, Bristol, pp 54-70
14. Griffiths G (1993) Fine structure immunocytochemistry. Springer, Berlin Heidelberg New York
15. Gu A, D'Andrea M (1989) Comparison of detecting sensitivities of different sizes of gold particles with electron microscopic immunogold staining using atrial natriuretic peptide in rat atria as a model. Am J Anat 185:264-270
16. Hayat MA (1989) Colloidal gold: principles, methods and applications, vols 1 and 2. Academic Press, Orlando
17. Hayat MA (ed) (1995) Immunogold-silver staining: principles, methods, and applications. CRC Press, Boca Raton
18. Heitzmann H, Richards FM (1974) Use of avidin-biotin complex for specific staining of biological membranes in electron microscopy. Proc Natl Acad Sci USA 71:3537-3541
19. Hermann R, Walther P, Müller M (1996) Immunogold labeling in scanning electron microscopy. Histochem Cell Biol 106:31-39
20. Hodges GM, Southgate J, Toulson EC (1987) Colloidal gold - a powerful tool in SEM immunocytochemistry: an overview of bioapplications. Scanning Microsc 1:301-318
21. Holgate CS, Jackson P, Lauder I (1983) Surface membrane staining of immunoglobulins in paraffin sections of non-Hodgkin's lymphomas using immunogold-silver staining technique. J Clin Pathol 36:742-746
22. Horisberger M (1981) Colloidal gold: cytochemical marker for light and fluorescent microscopy and for transmission and scanning electron microscopy. Scanning Microsc 2:9-28
23. Horisberger M (1989) Colloidal gold for scanning electron microscopy. In: Hayat ME (ed) Colloidal gold: principles, methods and applications, vol 1. Academic Press, San Diego, pp 217-227
24. Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabelled antibody (PAP) procedures. J Histochem Cytochem 29:577-580
25. Humbel BM, Sibon OCM, Stierhof YD, Schwarz H (1995) Ultrasmall gold particles and silver enhancement as a detection system in immunolabeling and in situ hybridization experiments. J Histochem Cytochem 43:735-737
26. Kerstens JMJ, Poddighe PJ, Hanselaar AGJM (1995) A novel in situ hybridization signal amplification method, based on the deposition of biotinylated tyramine. J Histochem Cytochem 43:347-352
27. Lackie PM (1996) Immunogold-silver staining for light microscopy. Histochem Cell Biol 106:9-17
28. Lackie PM, Hennessy RJ, Hacker GW, Polak JM (1985) Investigation of immunogold silver staining by electron microscopy. Histochemistry 83:545-550
29. Lah JJ, Hayes DM, Burry RW (1990) A neutral pH silver development method for the visualization of 1-nm gold particles in pre-embedding electron microscopic immunocytochemistry. J Histochem Cytochem 38:503-508
30. Merz H, Malisius R, Mannweiler S, Zhou R, Hartmann W, Orscheseck K, Moubayed P, Feller A (1995) Methods in laboratory investigation. Immunomax: a maximized immunohistochemical method for the retrieval and enhancement of hidden antigens. Lab Invest 73:149-156
31. Müller-Höcker J, Schäfer S (1996) Cytochemistry of cytochrome-c oxidase at the electron microscope level. Methods Enzymol 264:540-555
32. Plas P van de, Leunissen JCM (1994) Ultrasmall gold probes: characteristics and use in immuno(cyto)chemical studies. In: Sia DJ (ed) Methods in cell biology. Academic Press, San Diego, pp 241-257
33. Polak JM, Priestley JV (1992) Electron microscopic immunocytochemistry. Principles and practice. University Press, Oxford
34. Roth J (1984) The protein A-gold technique for antigen localization in tissue sections by light and electron microscopy. In: Polak J, Varndell IM (eds.) Immunolabelling for electron microscopy. Elsevier, Amsterdam, pp 113-122
35. Roth J (1986) Postembedding cytochemistry with gold labelling reagents: a review. J Microsc 143:125-137
36. Roth J, Saremaslani P, Warhol MJ (1992) Improved accuracy in diagnostic immunohistochemistry, lectin histochemistry and in situ hybridization using a gold-labeled horseradish peroxidase antibody and silver intensification. Lab Invest 67:263-269
37. Scopsi L (1989) In: Hayat MA (ed) Colloidal gold: principles, methods, and applications, vol 1. Academic Press, San Diego, pp 251-295
38. Scopsi L, Larsson LI, Bastholm L, Nielsen MH (1986) Silver enhances colloidal gold probes as markers for scanning electron microscopy. Histochemistry 86:35-42
39. Slot JW, Geuze HJ (1984) Gold markers for single and double immunolabeling of ultrathin cryosections. In: Polak J, Varndell IM (eds) Immunolabeling for electron microscopy. Elsevier, Amsterdam, pp 129-142
40. Stierhof YD, Humbel BM, Schwarz H (1991) Suitability of different silver enhancement methods applied to 1-nm colloidal gold particles: an immunoelectronmicroscopic study. J Electron Microsc Tech 17:336-343
41. Stierhof YD, Humbel BM, Hermann R, Otten MT, Schwarz H (1992) Direct visualization and silver enhancement of ultra-small antibody-bound gold particles of immunolabeled ultra-thin resin sections. Scanning Microsc 6:1009-1022
42. Stirling JW (1990) Immuno- and affinity probes for electron microscopy: a review of labelling and preparations techniques. J Histochem Cytochem 38:145-158
43. Takizawa T, Robinson JM (1994) Use of 1.4-nm immunogold particles for immunocytochemistry on ultra-thin cryosections. J Histochem Cytochem 42:1615-1623
44. Van Bergen Henegouwen PMP, Leunissen JLM (1986) Controlled growth of colloidal gold particles for labeling efficiency. Histochemistry 85:81-87
45. Verkely AJ, Leunissen JLM (eds) (1989) Immunogold Labelling. CRC Press, Boca Raton
46. Viale G, Dell'Orto P, Braidotti P, Goggi G (1985) Ultrastructural localization of intracellular immunoglobulins in Epon-embedded human lymph nodes. An immunoelectron microscopic investigation using the immunogold staining (IGS) and the avidin-biotin-peroxidase complex (ABC) methods. J Histochem Cytochem 33:400-406
47. Werner M, Wasielewski R von, Komminoth P (1996) Antigen retrieval, signal amplification and intensification in immunohistochemistry. Histochem Cell Biol 105:253-260
48. Yokota S (1988) Effect of particle size on labeling density of catalase in protein A-gold immunocytochemistry. J Histochem Cytochem 36:107-109