A mimic as internal standard to monitor PCR analysis of food-borne pathogens

Letters in Applied Microbiology

Volumn 26, Issue 1, 1998, Pages 9-11

  Lambertz, S Thisted ^a,b   Ballagi Pordany A ^a   Lindqvist, R ^a  

^a SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^b NATIONAL FOOD ADMINISTRATION   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL NUMBERS; FALSE NEGATIVES; FOOD-BORNE PATHOGENS; INTERNAL STANDARDS; PCR ANALYSIS; TARGET CONCENTRATIONS; YERSINIA ENTEROCOLITICA;

DNA FRAGMENT;

ARTICLE; BACTERIUM DETECTION; DIAGNOSTIC ERROR; FOOD ANALYSIS; NONHUMAN; POLYMERASE CHAIN REACTION; STANDARD; YERSINIA ENTEROCOLITICA;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; YERSINIA ENTEROCOLITICA;

EID: 0031907013     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1472-765X.1998.00256.x     Document Type: Article

References (10)

1. Ballagi-Pordány, A. and Belák, S. (1996) The use of mimics as internal standards to avoid false negatives in diagnostic PCR. Molecular and Cellular Probes 10, 159-164.
2. Fenwick, S.G. and Murray, A. (1991) Detection of pathogenic Yersinia enterocolitica by polymerase chain reaction. Lancet 337, 496-497.
3. Jin, C.-F., Mata, M. and Fink, D.J. (1994) Rapid construction of deleted DNA fragments for use as internal standards in competitive PCR. PCR Methods and Applications 3, 252-255.
4. Lambertz, S.T., Ballagi-Pordány, A., Nilsson, A., Norberg, P. and Danielsson-Tham, M.-L. (1996) Comparison of a PCR method and a conventional culture method for detection of pathogenic Yersinia enterocolitica in food. Journal of Applied Bacteriology 81, 303-308.
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8. Rossen, L., Nörskov, P., Holmström, K. and Rasmussen, O.F. (1992) Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions. International Journal of Food Microbiology 17, 37-45.
9. Siebert, P.D. and Larrick, J.W. (1993) PCR MIMICS: competitive DNA fragments for use as internal standards in quantitative PCR. BioTechniaues 14, 24-29.
10. Wren, B.W. and Tabaqchali, S. (1990) Detection of pathogenic Yersinia enterocolitica by the polymerase chain reaction. Lancet 336, 693.