Expression and rapid purification of an Aedes aegypti salivary allergen by a baculovirus system

International Archives of Allergy and Immunology

Volumn 115, Issue 3, 1998, Pages 245-251

  Xu, Wenzong ^a   Simons, F Estelle R ^a   Peng, Zhikang ^a,b  

^a UNIVERSITY OF MANITOBA   (Canada)

^b NONE   (Canada)

Author keywords

Aed a 1; Aedes aegypti; Baculovirus; Mosquito allergy; Purification; Recombinant allergen antigen

Indexed keywords

ALLERGEN; APYRASE;

AEDES AEGYPTI; AFFINITY CHROMATOGRAPHY; ARTICLE; BACULOVIRUS; EXPRESSION VECTOR; IMMUNOBLOTTING; INSECT ALLERGY; MOLECULAR CLONING; PRIORITY JOURNAL; PURIFICATION;

AEDES; ALLERGENS; ANIMALS; BACULOVIRIDAE; CELLS, CULTURED; CHROMATOGRAPHY, AFFINITY; GENE EXPRESSION; HISTIDINE; HUMANS; HYPERSENSITIVITY, IMMEDIATE; IMMUNOBLOTTING; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; PLASMIDS; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; SALIVARY PROTEINS;

EID: 0031905832     PISSN: 10182438     EISSN: None     Source Type: Journal    
DOI: 10.1159/000023907     Document Type: Article

References (28)

1. Hudson A, Bowman L, Orr CWM: Effects of absence of saliva on blood feeding by mosquitoes. Science 1960;131:1730-1731.
2. Oka K: Correlation of Aedes albopictus bite reaction with IgE antibody assay and lymphocyte transformation test to mosquito salivary antigens. J Dermatol 1989;16:341-347.
3. Peng Z, Yang M, Simons FER: Immunologic mechanisms in mosquito allergy: Correlations of skin reactions with specific IgE and IgG antibodies and lymphocyte proliferation response to mosquito antigens. Ann Allergy Asthma Immunol 1996;77:238-244.
4. McCormack DR, Salata KF, Hershey JN, Carpenter GB, Engler RJ: Mosquito bite anaphylaxis: immunotherapy with whole body extracts. Ann Allergy Asthma Immunol 1995;74: 39-44.
5. Benaim-Pinto C, Fassrainer A: Intradermal immunotherapy in children with severe skin inflammatory reactions to Aedes aegypti and Culex quinquefasciatus mosquito bites. Int J Dermatol 1990;29:600-601.
6. Lelong M: Severe local reactions to mosquito bites: A propos of 20 cases. Allerg Immunol (Paris) 1986;18:21-22.
7. Peng Z, Simons FER: Comparison of proteins, IgE and IgG binding antigens and skin reactivity in commercial and laboratory-made mosquito extracts. Ann Allergy Asthma Immunol 1996;77:371-376.
8. Rafnar T, Ghosh B, Metzler WJ, Huang SK, Perry MP, Mueller L, Marsh DG: Expression and analysis of recombinant Amb a V and Amb t V allergens. J Biol Chem 1992;267:21119-21123.
9. Rogers BL, Morgenstern JP, Garman RD, Bond JF, Kuo MC: Recombinant Fel d 1: expression, purification, IgE binding and reaction with catallergic human T cells. Mol Immunol 1993;30: 559-568.
10. Tamborini E, Brandazza A, De Lalla C, Musco G, Siccardi AG, Arosio P, Sidoli A: Recombinant allergen Lol p II: Expression, purification and characterization. Mol Immunol 1995;32: 505-513.
11. Racioppi JV, Spielman A: Secretory proteins from the salivary glands of adult Aedes aegypti mosquitoes. Insect Biochem 1987;17:503-511.
12. Ribeiro JM, Sarkis JJ, Rossignol PA, Spielman A: Salivary apyrase of Aedes aegypti: Characterization and secretory fate. Comp Biochem Physiol [B] 1984;79:81-86.
13. Peng Z, Li H, Simons FER: Immunoblot analyses of salivary allergens in 10 mosquito species with world-wide distributions, and the IgE responses to these allergens. J Allergy Clin Immunol 1998, in press.
14. Larsen JN and Lowenstein H: Allergen nomenclature. J Allergy Clin Immunol 1996; 97:577-578.
15. Champagne DE, Smartt CT, Ribeiro JM, James AA: The salivary gland-specific apyrase of the mosquito Aedes aegypti is a member of the 5′-nucleotidase family. Proc Natl Acad Sci USA 1995;92:694-698.
16. Chen XS, Brash AR, Funk CD: Purification and characterization of recombinant histidinetagged human platelet 12-lipoxygenase expressed in a baculovirus/insect cell system. Eur J Biochem 1993;214:845-852.
17. Reddy RG, Yoshimoto T, Yamamoto S, Funk CD, Marnett LJ: Expression of porcine leukocyte 12-lipoxygenase in a baculovirus/insect cell system and its characterization. Arch Biochem Biophys 1994;312:219-226.
18. 558 allows reconstitution of the phagocyte NADPH oxidase solely from recombinant proteins. J Biol Chem 1993;268:14256-14260.
19. O'Reilly DR, Miller LK, Luckow VA: Baculovirus Expression Vectors: A Laboratory Manual. London, Oxford University Press, 1994.
20. Matsuura Y, Possee RD, Overton HA, Bishop DH: Baculovirus expression vectors: The requirements for high level expression of proteins, including glycoproteins. J Gen Virol 1987;68:1233-1250.
21. Rupp B, Rossler U, Lowel M, Werenskiold AK: High level expression of the IL-1 receptor related T1 receptor in insect cells. Biochem Biophys Res Commun 1995;216:595-601.
22. Chalkley GE, Knowles PP, Whitehead PC, Coffer AI: Biochemical characterisation of purified human wild-type p53 overexpressed in insect cells. Eur J Biochem 1994;221:167-175.
23. 2. Biochem Biophys Acta 1992;1165: 201-210.
24. Janssen JJM, Bovee-Geurts PHM, Merkx M, DeGrip WJ: Histidine tagging both allows convenient single-step purification of bovine rhodopsin and exerts ionic strength-dependent effects on its photochemistry. J Biol Chem 1995; 270:11222-11229.
25. Xu W, Simons FER, Peng Z: Expression and characterization of a recombinant major salivary protein of the mosquito Ae. aegypti. FASEB J 1996;10:A1479.
26. Greene WK, Cyster JG, Chua KY, O'Brien RM, Thomas WR: IgE and IgG binding of peptides expressed from fragments of cDNA encoding the major house dust mite allergen Der p I. J Immunol 1991;147:3768-3773.
27. Benson RL: Diagnosis and treatment of sensitization to mosquitoes. J Allergy 1936;8:47-59.
28. Newsome WH, Jones JKN, French FE, West AS: The isolation and properties of the skinreactive substance in Aedes aegypti oral secretion. Can J Biochem 1969;47:1129-1136.