Preparation of DNA extracted from environmental water samples for PCR amplification

Journal of Microbiological Methods

Volumn 31, Issue 3, 1998, Pages 193-199

  Boccuzzi, Victoria M ^a   Straube, William L ^a   Ravel, Jacques ^a   Colwell, Rita R ^a   Hill, Russell T ^a,b  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^b AUSTRALIAN INSTITUTE OF MARINE SCIENCE   (Australia)

Author keywords

Humics; PCR; Sephadex; Spun column

Indexed keywords

ESCHERICHIA COLI ENTEROTOXIN; SEPHADEX;

ARTICLE; CHROMATOGRAPHY; DNA DETERMINATION; DNA PURIFICATION; ENVIRONMENT; ESCHERICHIA COLI; EXTRACTION; GENE AMPLIFICATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; WATER ANALYSIS;

EID: 0031905423     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(97)00106-1     Document Type: Article

References (19)

1. [1] K.D. Bruce, D. Hiorns, J.L. Hobman, A.M. Osborn, P. Strike, D.A. Ritchie, Amplification of DNA from native populations of soil bacteria by using the PCR, Appl. Environ. Microbiol. 58 (1992) 3413-3416.
2. [2] S.D. Pillai, K.L. Josephson, R.L. Bailey, C.P. Gerba, I.L. Pepper, Rapid method for processing soil samples for polymerase chain reaction amplification of specific gene sequences, Appl. Environ. Microbiol. 57 (1991) 2283-2286.
3. [3] C.C. Somerville, I.T. Knight, W.L. Straube, R.R. Colwell, Simple, rapid method for direct isolation of nucleic acids from aquatic environments, Appl. Environ. Microbiol. 55 (1989) 548-554.
4. [4] C.C. Tebbe, W. Vahjen, Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast, Appl. Environ. Microbiol. 59 (1993) 2657-2665.
5. [5] J.T. Trevors, H. Lee, S. Cook, Direct detection of DNA from soil, Microb. Releases 1 (1992) 111-115.
6. [6] Y.-L. Tsai, B.H. Olson, Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction, Appl. Environ. Microbiol. 58 (1992) 2292-2295.
7. [7] C.S. Jacobsen, O.F. Rasmussen, Development and application of a new method to extract bacterial DNA from soil based on separation of bacteria from soil with cation-exchange resin, Appl. Environ. Microbiol. 58 (1992) 2458-2462.
8. [8] J.T. Trevors, J.D. van Elsas, 1995. Nucleic acids in the environment, Springer-Verlag, New York.
9. [9] I.G. Wilson, Inhibition and facilitation of nucleic acid amplification, Appl. Environ. Microbiol. 63 (1997) 3741-3751.
10. [10] L.G. Leff, J.R. Dana, J.V. McArthur, L.J. Shimkets, Comparison of methods of DNA extraction from stream sediments, Appl. Environ. Microbiol. 61 (1995) 1141-1143.
11. [11] S.D. Michaels, M.C. John, R.M. Amasino, Removal of polysaccharides from plant DNA by ethanol precipitation, BioTechniques 17 (1994) 274-276.
12. [12] A. Ogram, G.S. Sayler, T. Barkay, The extraction and purification of microbial DNA from sediments, J. Microbiol. Methods 7 (1987) 57-66.
13. [13] W.E. Holben, J.K. Jansson, B.K. Chelm, J.M. Tiedje, DNA probe method for the detection of specific microorganisms in the soil bacterial community, Appl. Environ. Microbiol. 54 (1988) 703-711.
14. [14] Pharmacia Gel filtration: Principles and methods. Rahms I Lund, Uppsala, Sweden, 1991.
15. [15] R.A. Levine, Aquarium filter floss: an alternative to silanized glass wool as a porous support matrix, BioTechniques 17 (1994) 67.
16. [16] J.E. Hobbie, R.J. Daley, S. Jasper, Use of nucleopore filters for counting bacteria by fluorescence microscopy, Appl. Environ. Microbiol. 33 (1977) 1225-1228.
17. [17] A.C. Wright, Y. Guo, J.A. Johnson, J.P. Nataro, J.G. Morris Jr., Development and testing of a nonradioactive DNA oligonucleotide probe that is specific for Vibrio cholerae cholera toxin, J. Clin. Microbiol. 30 (1992) 2302-2306.
18. [18] A.L. Lang, Y.-L. Tsai, C.L. Mayer, K.C. Patton, C.J. Palmer, Multiplex PCR detection of the heat-labile toxin gene and shiga-like Toxin I and Toxin II genes in Escherichia coli isolated from natural waters, Appl. Environ. Microbiol. 60 (1994) 3145-3149.
19. [19] J.E. Sambrook, E.F. Fritsch, T. Maniatis, 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.