A novel MspI PCR-RFLP in the human cytosine 5-methyltransferase gene: Lack of relevance for malignant lymphoproliferative disease and breast cancer

Human Heredity

Volumn 48, Issue 4, 1998, Pages 226-229

  Bagwe, Aparna N ^a   Ahmed, Mohammed O ^a   Franchina, M ^a   Spagnolo, Dominic V ^b   Papadimitriou, John M ^a   Kay, Peter H ^a  

^a UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

^b Western Australian Centre for Pathology and Medical Research   (Australia)

Author keywords

Human cytosine 5 methyltransferase; Intron; Malignancy; MspI site; Polymorphism

Indexed keywords

CYTOSINE; ISOENZYME; METHYLTRANSFERASE;

ARTICLE; BREAST CANCER; CONTROLLED STUDY; GENE NUMBER; GENETIC POLYMORPHISM; GENOTYPE; HUMAN; HUMAN CELL; INTRON; LYMPHOPROLIFERATIVE DISEASE; MAJOR CLINICAL STUDY; NONHODGKIN LYMPHOMA; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

ALLELES; BREAST NEOPLASMS; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA (CYTOSINE-5-)-METHYLTRANSFERASE; FEMALE; GENE DOSAGE; GENE FREQUENCY; HUMANS; INTRONS; LYMPHOMA, NON-HODGKIN; MALE; PHENOTYPE; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SITE-SPECIFIC DNA METHYLTRANSFERASE (CYTOSINE-SPECIFIC);

EID: 0031903442     PISSN: 00015652     EISSN: None     Source Type: Journal    
DOI: 10.1159/000022805     Document Type: Article

References (10)

1. pG methylation in mammalian cells. Nat Genet 1995;10:20-27.
2. Klimasauskas S, Kumar S, Roberts RJ, Cheng X: Hhal methyltransferase flips its target base out of the DNA helix. Cell 1994;76:357-369.
3. Wu J, Issa J-P, Herman J, Bassett DE Jr, Nelkin BD, Baylin SB: Expression of an exogenous eukaryotic DNA methyltransferase gene induces transformation of NIH 3T3 cells. Proc Natl Acad Sci USA 1993; 90:8891-8895.
4. Laird PW, Jackson-Grusby L, Fazeli A, Dickinson SL, Jung WE, Li E, Weinberg RA, Jaenisch R: Suppression of intestinal neoplasia by DNA hypomethylation. Cell 1995;81: 197-205.
5. Kay PH, Spagnolo DV, Taylor J, Ziman M: DNA methylation and developmental genes in lymphomagenesis - more questions than answers? Leuk Lymphoma 1997;24: 211-220.
6. Hähnel R, Harvey J, Kay PH: Hypermethylation of the myogenic gene MYF-3 in human breast carcinomas. Anticancer Res 1996; 16:2111-2116.
7. Maniatis T, Fritsch EF, Sambrook J: Molecular cloning: a laboratory manual. New York, Cold Spring Harbor Laboratories, 1982.
8. Southern EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975;95:503-509.
9. Yen R-WC, Vertino PM, Nelkin BD, Yu JJ, El-Deiry W, Cumaraswamy A, Lennon GG, Trask BJ, Celano P, Baylin SB: Isolation and characterization of the cDNA encoding human DNA methyltransferase. Nucleic Acids Res 1992;20: 2297-2291.
10. Bestor T, Laudano A, Mattaliano R, Ingram V: Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cells. J Mol Biol 1988;203:971-983.