Deletion analysis of human AP-endonuclease: Minimum sequence required for the endonuclease activity

Carcinogenesis

Volumn 19, Issue 3, 1998, Pages 525-527

  Izumi, Tadahide ^a   Mitra, Sankar ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; DNA (APURINIC OR APYRIMIDINIC SITE) LYASE;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DELETION MUTANT; DNA ALKYLATION; DNA DAMAGE; ENZYME ACTIVITY; ESCHERICHIA COLI; EXCISION REPAIR; NONHUMAN; PRIORITY JOURNAL;

CARBON-OXYGEN LYASES; DEOXYRIBONUCLEASE IV (PHAGE T4-INDUCED); DNA DAMAGE; DNA REPAIR; DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HUMANS; OXIDATION-REDUCTION; PHENOTYPE; RECOMBINANT PROTEINS; SEQUENCE DELETION; TRANSCRIPTION, GENETIC;

EID: 0031901923     PISSN: 01433334     EISSN: None     Source Type: Journal    
DOI: 10.1093/carcin/19.3.525     Document Type: Article

References (16)

1. Barzilay, G., Walker, L.J., Robson, C.N. and Hickson, I.D. (1995) Site-directed mutagenesis of the human DNA repair enzyme HAP1: identification of residues important for AP endonuclease and RNase H activity. Nucleic Acids Res., 23, 1544-1550.
2. Cunningham, R.P., Saporito, S.M., Spitzer, S.G. and Weiss, B. (1986) Endonuclease IV (nfo) mutant of Escherichia coli. J. Bacteriol., 168, 1120-1127.
3. Demple, B., Herman, T. and Chen, D.S. (1991) Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes. Proc. Natl Acad. Sci. USA, 88, 11450-11454.
4. Doetsch, P.W. and Cunningham, R.P. (1990) The enzymology of apurinic/ apyrimidinic endonucleases. Mutat. Res., 236, 173-201.
5. Hirota, K., Matsui, M., Iwata, S., Nishiyama, A., Mori, K., and Yodoi, J. (1997) AP-1 transcriptional activity is regulated by a direct association between thioredoxin and Ref-1. Proc. Natl Acad. Sci. USA, 94, 3633-3638.
6. Izumi, T., Henner, W.D., and Mitra, S. (1996) Negative regulation of the major human AP-endonuclease, a multifunctional protein. Biochemistry, 35, 14679-14683.
7. Mol, C.D., Kuo, C.F., Thayer, M.M., Cunningham, R.P. and Tainer, J.A. (1995) Structure and function of the multifunctional DNA-repair enzyme exonuclease III. Nature, 374, 381-386.
8. Okazaki, T., Chung, U., Nishishita, T., Ebisu, S., Usuda, S., Mishiro, S., Xanthoudakis, S., Igarashi, J. and Ogata, E. (1994) A redox factor protein, ref1, is involved in negative gene regulation by extracellular calcium. J. Biol. Chem., 269, 27855-27862.
9. Qin, J., Clore, G.M., Kennedy, W.P., Kuszewski, J., and Gronenborn, A.M. (1996) The solution structure of human thioredoxin complexed with its target from Ref-1 reveals peptide chain reversal. Structure, 4, 613-620.
10. Robson, C.N. and Hickson, I.D. (1991) Isolation of cDNA clones encoding a human apurinic/apyrimidinic endonuclease that corrects DNA repair and mutagenesis defects in E.coli xth (exonuclease III) mutants. Nucleic Acids Res., 19, 5519-5523.
11. Seki, S., Hatsushika, M., Watanabe, S., Akiyama, K., Nagao, K. and Tsutsui, K. (1992) cDNA cloning, sequencing, expression and possible domain structure of human APEX nuclease homologous to Escherichia coli exonuclease III. Biochim. Biophys. Acta, 1131, 287-299.
12. Walker, L.J., Robson, C.N., Black, E., Gillespie, D. and Hickson, I.D. (1993) Identification of residues in the human DNA repair enzyme HAP1 (Ref-1) that are essential for redox regulation of Jun DNA binding. Mol. Cell. Biol., 13, 5370-5376.
13. Wallace, S. (1994) DNA damages processed by base excision repair: biological consequences. Int. J. Radiat. Biol., 66, 579-589.
14. Xanthoudakis, S., Miao, G., Wang, F., Pan, Y.C. and Curran, T. (1992) Redox activation of Fos-Jun DNA binding activity is mediated by a DNA repair enzyme. EMBO J., 11, 3323-3335.
15. Xanthoudakis, S., Miao, G.G. and Curran, T. (1994) The redox and DNA-repair activities of Ref-1 are encoded by nonoverlapping domains. Proc. Natl Acad. Sci. USA, 91, 23-27.
16. Xanthoudakis, S., Smeyne, R.J., Wallace, J.D. and Curran, T. (1996) The redox/DNA repair protein, Ref-1, is essential for early embryonic development in mice. Proc. Natl Acad. Sci. USA, 93, 8919-8923.