Mutational analysis of the phosphate-binding loop of Rhizobium meliloti DctD, a σ54-dependent activator

Journal of Bacteriology

Volumn 180, Issue 10, 1998, Pages 2792-2795

  Gao, Yan ^a   Wang, Ying Kai ^a   Hoover, Timothy R ^a  

^a UNIVERSITY OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; PHOSPHATE BINDING AGENT;

AMINO ACID SUBSTITUTION; ARTICLE; DNA BINDING; MUTATION RATE; NONHUMAN; PRIORITY JOURNAL; SINORHIZOBIUM MELILOTI; TRANSCRIPTION INITIATION;

NEGIBACTERIA; PROKARYOTA; RHIZOBIUM; RHIZOBIUM MELILOTI; SINORHIZOBIUM MELILOTI;

EID: 0031899313     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.10.2792-2795.1998     Document Type: Article

References (30)

1. Austin, S., C. Kundrot, and R. Dixon. 1991. Influence of a mutation in the putative nucleotide binding site of the nitrogen regulatory protein NTRC on its positive control function. Nucleic Acids Res. 19:2281-2287.
2. Bolton, E., B. Higgisson, A. Harrington, and F. O'Gara. 1986. Dicarboxylic acid transport in Rhizobium meliloti: isolation of mutants and cloning of dicarboxylic acid transport genes. Arch. Microbiol. 144:142-146.
3. Buck, M., W. Cannon, and J. Woodcock. 1987. Transcriptional activation of the Klebsiella pneumoniae nitrogenase promoter may involve DNA loop formation. Mol. Microbiol. 1:243-249.
4. Cannon, W., and M. Buck. 1992. Central domain of the positive control protein NifA and its role in transcriptional activation. J. Mol. Biol. 225:271-286.
5. 2+ binding site. J. Mol. Biol. 249:654-664.
6. Cunningham, B., and J. Wells. 1989. High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis. Science 244: 1081-1085.
7. 4-dicarboxylate carrier. J. Bacteriol. 171:5551-5560.
8. Jiang, J., B. Gu, L. M. Albrigbt, and B. T. Nixon. 1989. Conservation between coding and regulatory elements of Rhizobium meliloti and Rhizobium leguminosarum dct genes. J. Bacteriol. 171:5244-5253.
9. Ledebur, H., and B. T. Nixon. 1992. Tandem DctD-binding sites of the Rhizobium meliloti dctA upstream activating sequence are essential for optimal function despite a 50- to 100-fold difference in affinity for DctD. Mol. Microbiol. 6:3479-3492.
10. Lee, H.-S., D. K. Berger, and S. Kustu. 1993. Activity of purified NIFA, a transcriptional activator of nitrogen fixation genes. Proc. Natl. Acad. Sci. USA 90:2266-2270.
11. 54 and the β subunit of RNA polymerase. Proc. Natl. Acad. Sci. USA 92:9702-9706.
12. 54-dependent transcriptional activator. J. Biol. Chem. 269:20401-20409.
13. 54 with the Klebsiella pneumoniae and Rhizobium meliloti nifH promoters: the role of NIFA in the formation of an open promoter complex. J. Mol. Biol. 210:65-77.
14. 54-holoenzyme. J. Mol. Biol. 260:317-331.
15. 54-dependent activator XylR triggers a protein multimerization cycle catalyzed by UAS DNA. Cell 86:331-339.
16. Popham, D., D. Szeto, J. Keener, and S. Kustu. 1989. Function of a bacterial activator protein that binds to transcriptional enhancers. Science 243:629-635.
17. 54 holoenzyme by scanning force microscopy. J. Mol. Biol. 270:125-138.
18. Ronson, C. W., P. M. Astwood, B. T. Nixon, and F. M. Ausubel. 1987. Deduced products of C4-dicarboxylate transport regulatory genes of Rhizobium leguminosarum are homologous to nitrogen regulatory gene products. Nucleic Acids Res. 15:7921-7934.
19. Saraste, M., P. R. Sibbald, and A. Wittinghofer. 1990. The P-loop - a common motif in ATP- and GTP-binding proteins. Trends Biol. Sci. 15:430-434.
20. Sasse-Dwight, S., and J. D. Gralla. 1988. Probing the Escherichia coli glnALG upstream activation mechanism in vivo. Proc. Natl. Acad. Sci. USA 85:8934-8938.
21. Scholl, D., and B. T. Nixon. 1996. Cooperative binding of DctD to the dctA UAS of Rhizobium meliloti is enhanced in a constitutively active truncated mutant. J. Biol. Chem. 271:26435-26442.
22. 1-ATPase from yeast. J. Biol. Chem. 269:9424-9428.
23. Smith, C. A., and I. Rayment. 1996. Active site comparisons highlight structural similarities between myosin and other P-loop proteins. Biophys. J. 70:1590-1602.
24. Su, W., S. Porter, S. Kustu, and H. Echols. 1990. DNA-looping and enhancer activity: association between DNA-bound NTRC activator and RNA polymerase at the bacterial glnA promoter. Proc. Natl. Acad. Sci. USA 87:5504-5508.
25. Walker, J. E., M. Saraste, M. J. Runswick, and N. J. Gay. 1982. Distantly related sequences in the α- and β-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1:945-951.
26. 54-dependent activator DctD that prevent transcriptional activation. J. Bacteriol. 179:5812-5819.
27. 54-dependent activator DctD is involved in both binding to RNA polymerase and coupling ATP hydrolysis to activation. Mol. Microbiol. 26:373-386.
28. Wedel, A., D. S. Weiss, D. Popham, P. Droge, and S. Kustu. 1990. A bacterial enhancer functions to tether a transcriptional activator near a promoter. Science 248:486-490.
29. Wedel, A. B., and S. Kustu. 1995. The bacterial enhancer-binding protein NtrC is a molecular machine: ATP hydrolysis is coupled to transcriptional activation. Genes Dev. 9:2042-2052.
30. Weiss, D. S., J. Batut, K. E. Klose, J. Keener, and S. Kustu. 1991. The phosphorylated form of the enhancer-binding protein NTRC has an ATPase activity that is essential for activation of transcription. Cell 67:155-167.