The N-terminal domain of IκBα masks the nuclear localization signal(s) of p50 and c-Rel homodimers

Molecular and Cellular Biology

Volumn 18, Issue 5, 1998, Pages 2640-2649

  Latimer, Matthew ^a   Ernst, Mary K ^a   Dunn, Linda L ^a   Drutskaya, Marina ^a   Rice, Nancy R ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANKYRIN; DNA BINDING PROTEIN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; TRANSCRIPTION FACTOR;

AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; DIMERIZATION; HUMAN; HUMAN CELL; MOLECULAR INTERACTION; PRIORITY JOURNAL; PROTEIN DNA BINDING; TRANSCRIPTION REGULATION;

EID: 0031897457     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/mcb.18.5.2640     Document Type: Article

References (31)

1. Baldwin, A. S., Jr. 1996. The NF-κB and IκB proteins: new discoveries and insights. Annu. Rev. Immunol. 14:649-681.
2. Beg, A. A., S. M. Ruben, R. I. Scheinman, S. Haskill, C. A. Rosen, and A. S. Baldwin, Jr. 1992. IκB interacts with the nuclear localization sequences of the subunits of NF-κB: a mechanism for cytoplasmic retention. Genes Dev. 6:1899-1913.
3. Beisel, C. E., J. F. Edwards, L. L. Dunn, and N. R. Rice. 1993. Analysis of multiple mRNAs from pathogenic equine infectious anemia virus (EIAV) in an acutely infected horse reveals a novel protein, Ttm, derived from the carboxy terminus of the EIAV transmembrane protein. J. Virol. 67:832-842.
4. Brockman, J., D. Scherer, T. McKinsey, S. Hall, X. Qi, W. Lee, and D. Ballard. 1995. Coupling of a signal response domain in IκBα to multiple pathways for NF-κB activation. Mol. Cell. Biol. 15:2809-2818.
5. Brown, K., S. Gerstberger, L. Carlson, G. Franzoso, and U. Siebenlist. 1995. Control of IκBα proteolysis by site-specific signal-induced phosphorylation. Science 267:1485-1488.
6. Chen, F. E., D.-B. Huang, Y.-Q. Chen, and G. Ghosh. 1998. Crystal structure of p50/p65 heterodimer of transcription factor NF-κB bound to DNA. Nature 391:410-413.
7. Chen, Y.-Q., S. Ghosh, and G. Ghosh. 1998. A novel DNA recognition mode by the NF-κB p65 homodimer. Nat. Struct. Biol. 5:67-73.
8. Chen, Z., J. Hagler, V. Palombella, F. Melandri, D. Scherer, D. Ballard, and T. Maniatis. 1995. Signal-induced site-specific phosphorylation targets IκBα to the ubiquitin-proteasome pathway. Genes Dev. 9:1586-1597.
9. Ernst, M. K., L. L. Dunn, and N. R. Rice. 1995. The PEST-like sequence of IκBα is responsible for inhibition of DNA binding but not for cytoplasmic retention of c-Rel or RelA homodimers. Mol. Cell. Biol. 15:872-882.
10. Ganchi, P. A., S.-C. Sun, W. C. Greene, and D. W. Ballard. 1992. IκB/MAD-3 masks the nuclear localization signal of NF-κB p65 and requires the transactivation domain to inhibit NF-κB p65 DNA binding. Mol. Biol. Cell 3:1339-1352.
11. Ghosh, G., G. Va Duyne, S. Ghosh, and P. Sigler. 1995. Structure of NF-κB p50 homodimer bound to a κB site. Nature 373:303-310.
12. Govind, S., E. Drier, L. H. Huang, and R. Steward. 1996. Regulated nuclear import of the Drosophila Rel protein dorsal: structure-function analysis. Mol. Cell. Biol. 16:1103-1114.
13. Hatada, E. N., M. Naumann, and C. Scheidereit. 1993. Common structural constituents confer IκB activity to NF-KB p105 and IκB/MAD-3. EMBO J. 12:2781-2788.
14. Inoue, J.-I., L. D. Kerr, D. Rashid, N. Davis, H. R. Bose, Jr., and I. M. Verma. 1992. Direct association of pp40/IκBβ with rel/NF-κB transcription factors: role of ankyrin repeats in the inhibition of DNA binding activity. Proc. Natl. Acad. Sci. USA 89:4333-4337.
15. Isoda, K., and C. Nüsslein-Volhard. 1994. Disulfide cross-linking in crude embryonic lysates reveals three complexes of the Drosophila morphogen dorsal and its inhibitor cactus. Proc. Natl. Acad. Sci. USA 91:5350-5354.
16. Kumar, S., and C. Gelinas. 1993. IκBα-mediated inhibition of v-Rel DNA binding requires direct interaction with the RxxRxRxxC Rel/κB DNA-binding motif. Proc. Natl. Acad. Sci. USA 90:8962-8966.
17. Lehming, N., S. McGuire, J. M. Brickman, and M. Ptashne. 1995. Interactions of a Rel protein with its inhibitor. Proc. Natl. Acad. Sci. USA 92:10242-10246.
18. Léveillard, T., and I. M. Verma. 1993. Diverse molecular mechanisms of inhibition of NF-κB/DNA binding complexes by IκB proteins. Gene Exp. 3:135-150.
19. Michaely, P., and V. Bennett. 1993. The membrane-binding domain of ankyrin contains four independently folded subdomains, each comprised of six ankyrin repeats. J. Biol. Chem. 268:22703-22709.
20. Miyamoto, S., and I. M. Verma. 1995. Rel-NF-κB/IκB story. Adv. Cancer Res. 66:255-292.
21. Muller, C., F. Rey, M. Sodeoka, G. Verdine, and S. Harrison. 1995. Structure of the NF-κB p50 homodimer bound to DNA. Nature 373:311-317.
22. Rice, N. R., M. L. MacKichan, and A. Israël. 1992. The precursor of NF-κB has IκB-like functions. Cell 71:243-253.
23. Rogers, S., R. Wells, and M. Rechsteiner. 1986. Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis. Science 234: 364-368.
24. Rottjakob, E. M., S. Sachdev, C. A. Leanna, T. A. McKinsey, and M. Hannink. 1996. PEST-dependent cytoplasmic retention of v-Rel by IκBα: evidence that IκB-α regulates cellular localization of c-Rel and v-Rel by distinct mechanisms. J. Virol. 70:3176-3188.
25. Tan, T.-H., G. P. Huang, A. Sica, P. Ghosh, H. A. Young, D. L. Longo, and N. R. Rice. 1992. κB site-dependent activation of the interleukin-2 receptor a-chain gene promoter by human c-Rel. Mol. Cell. Biol. 12:4067-4075.
26. Tatei, K., and M. Levine. 1995. Specificity of rel-inhibitor interactions in Drosophila embryos. Mol. Cell. Biol. 15:3627-3634.
27. Thompson, J. E., R. J. Phillips, H. Erdjument-Bromage, P. Tempst, and S. Ghosh. 1995. IκBβ regulates the persistent response in a biphasic activation of NF-κB. Cell 80:573-582.
28. Traenckner, E., H. Pahl, T. Henkel, K. Schmidt, S. Wilk, and P. Baeuerle. 1995. Phosphorylation of human IκBα on serines 32 and 36 controls IκBα proteolysis and NF-KB activation in response to diverse stimuli. EMBO J. 14:2876-2883.
29. Whiteside, S. T., J.-C. Epinat, N. R. Rice, and A. Israel. 1997. I kappa B epsilon, a novel member of the IκB family, controls RelA and cRel NF-κB activity. EMBO J. 16:1413-1426.
30. Whiteside, S. T., M. K. Ernst, O. LeBail, C. Laurent-Winter, N. Rice, and A. Israël. 1995. N- and C-terminal sequences control degradation of MAD3/IκBα in response to inducers of NF-κB activity. Mol. Cell. Biol. 15:5339-5345.
31. Wu, B.-Y., C. Woffendin, I. MacLachlan, and G. J. Nabel. 1997. Distinct domains of IκB-α inhibit human immunodeficiency virus type 1 replication through NF-κB and Rev. J. Virol. 71:3161-3167.