Heat-Shock-Induced Assembly of Hsp30 Family Members into High Molecular Weight Aggregates in Xenopus laevis Cultured Cells

Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

Volumn 119, Issue 2, 1998, Pages 381-389

  Ohan, Nicholas W ^a   Tam, Ying ^a   Heikkila, John J ^a  

^a UNIVERSITY OF WATERLOO   (Canada)

Author keywords

A6 cells; Heat shock protein; hsp70; Pore exclusion limit electrophoresis; Rate zonal centrifugation; Xenopus laevis

Indexed keywords

HEAT SHOCK PROTEIN; HEAT SHOCK PROTEIN 30; HEAT SHOCK PROTEIN 70; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; DENSITY GRADIENT CENTRIFUGATION; HEAT SHOCK; IMMUNOBLOTTING; KIDNEY EPITHELIUM; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; XENOPUS LAEVIS;

XENOPUS LAEVIS;

EID: 0031895813     PISSN: 03050491     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0305-0491(97)00364-7     Document Type: Article

References (32)

1. Anderson, L.; Borg, H.; Mikaelsson, M. Molecular weight estimations of proteins by electrophoresis in polyacrylamide gels of graded porosity. FEBS Lett. 20:199-202;1972.
2. Arrigo, A-P.; Landry, J. Expression and function of the low-molecular-weight heat shock proteins. In: Morimoto, R.I.; Tissieres, A.; Georgopolous, C. (eds). The Biology of Heat Shock Proteins and Molecular Chaperones. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1994:335-373.
3. Arrigo, A.-P.; Tanaka, K.; Goldberg, A. Identity of the 19S "prosome" particle with the large multifunctional protease complex of mammalian cells (the proteasome). Nature 331: 192-194;1988.
4. Arrigo, A-P.; Welch, W.J. Characterization and purification of the mammalian 28,000 dalton heat shock protein. J. Biol. Chem. 262:15359-15369;1987.
5. Atkinson, B.G.; Walden, D.B. Changes in Eukaryotic Gene Expression in Response to Environmental Stress. New York: Academic Press; 1985.
6. Bentley, N.J.; Fitch, I.T.; Tuite, M.F. The small heat-shock protein hsp26 of Saccharomyces cerevisiae assembles into a high molecular weight aggregate. Yeast 8:95-106;1992.
7. Collier, N.C.; Heuser, J.; Aach Levy, M.; Schlesinger, M.J. Ultrastructural and biochemical analysis of the stress granule in chicken embryo fibroblasts. J. Biol. Chem. 106:1131-1139; 1988.
8. Feige, U.; Morimoto, R.I.; Yahara, I.; Polla, B.S. Stress-Inducible Cellular Responses. Basel: Birkhauser Verllag Press; 1996.
9. Hartl, F.-U. Molecular chaperones in cellular protein folding. Nature 381:571-680;1996.
10. Heikkila, J.J.; Ohan, N.; Tam, Y.; Ali, A. Heat shock protein gene expression during Xenopus development. Cell. Mol. Life Sci. 53:114-121;1997.
11. Helm, K.; LaFayette, P.; Nagao, R.; Key, J.; Vierling, E. Localization of small heat shock proteins to the higher plant endomembrane system. Mol. Cell Biol. 13:238-247;1993.
12. Hockertz, M.K.; Clark-Lewis, I.; Candido, E.P.M. Studies of the small heat shock proteins of Caenorhabditis elegans using anti-peptide antibodies. FEBS Lett. 280:375-378;1991.
13. Kato, K.H.; Hasegawa, K.; Goto, S.; Inaguma, Y. Dissociation as a result of phosphorylation of an aggregated form of the small stress protein, hsp27. J. Biol. Chem. 269:11274-11278; 1994.
14. Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227:680-685; 1970.
15. Landry, J.; Chretien, P.; Lambert, H.; Hickey, E.; Weber, L.A. Heat shock resistance conferred by expression of the human hsp27 gene in rodent cells. J. Cell Biol. 109:7-15;1989.
16. Liang, P.; Amons, R.; Clegg, J.S.; MacRae, T.H. Purification, structure and molecular chaperone activity in vitro of Artemia p26, a small heat shock/α-crystallin protein. Eur. J. Biochem. 243:225-232;1997.
17. Lindquist, S.; Craig, E. The heat shock proteins. Annu. Rev. Genet. 22:631-677;1988.
18. McEwen, C.R. Tables for estimating sedimentation through linear concentration gradients of sucrose solution. Anal. Biochem. 20:114-1149;1967.
19. Mehlen, P.; Arrigo, A-P. The serum-induced phosphorylation of mammalian hsp27 correlates with changes in its intracellular localization and levels of oligomerization. Eur. J. Biochem. 221:327-334:1994.
20. Morimoto, R.I.; Tissieres, A.; Georgopoulos, C. The Biology of Heat Shock Proteins and Molecular Chaperones. New York: Cold Spring Harbor Laboratory Press; 1994.
21. Nover, L. The Heat Shock Response. Boca Raton, FL: CRC Press; 1991.
22. Nover, L.; Neumann, D.; Scharf, D. Intracellular localization and related functions of heat shock proteins. In: Nover, L. (ed). The Heat Shock Response. Boca Raton, FL: CRC Press; 1991:373-407.
23. Nover, L.; Scharf, D.; Neumann, D. Cytoplasmic heat shock granules are formed from precursor particles and contain a specific set of mRNAs. Mol. Cell. Biol. 9:1298-1308;1989.
24. Nover, L.; Scharf, D.; Neumann, D. Formation of cytoplasmic heat shock granules in tomato cell cultures and leaves. Mol. Cell. Biol. 3:1648-1655;1983.
25. O'Farrell, P. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250:4007-4021;1975.
26. Parsell, D.A.; Lindquist, S. The function of heat-shock proteins in stress tolerance: Degradation and reactivation of damaged proteins. Ann. Rev. Genet. 27:437-496;1993.
27. Schuldt, C.; Kloetzel, P-M. Analysis of cytoplasmic 19 S ringtype particles in Drosophila which contain hsp 23 at normal growth temperature. Dev. Biol. 110:65-72;1985.
28. Steensgarrd, J.; Moller, N.P.H.; Funding, L. Rate-zonal centrifugation: quantitative aspects. In: Bimie, G.D.; Rickwood, D. (eds). Centrifugal separations in molecular and cell biology. London: Butterworths Press; 1978:115-167.
29. Tam, Y.; Heikkila, J.J. Identification of members of the hsp30 small heat shock protein family and characterization of their developmental regulation in heat shocked Xenoptts laevis embryos. Dev. Gen. 17:331-339;1995.
30. White, C.N.; Hightower, L.E.; Schultz, R.J. Variation in heat-shock proteins among species of desert fishes (Poeciliidae, Poeciliopsis). Mol. Biol. Evol. 11:106-119;1994.
31. Winning, R.; Heikkila, J.J.; Bols, N. Induction of glucose-regulated proteins in Xenopus laevis A6 cells. J. Cell Physiol. 140:239-245;1989.
32. Young, B.D. Measurement of sedimentation coefficients and computer simulation of rate-zonal separations. In: Rickwood, D. (ed). Centrifugation: A Practical Approach. Oxford: IRL Press; 1984:127-159.