Molecular cloning of aspartic proteinases from flowers of Cynara cardunculus subsp. flavescens cv. sardoon and Centaurea calcitrapa

Advances in Experimental Medicine and Biology

Volumn 436, Issue , 1998, Pages 435-439

  Brodelius, P E ^a   Cordeiro, M ^a   Mercke, P ^a   Domingos, A ^b   Clemente, A ^b   Pais, M S ^c  

^a LUND UNIVERSITY   (Sweden)

^b INETI   (Portugal)

^c UNIVERSITY OF LISBON   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC PROTEINASE; PLANT DNA;

AMINO ACID SEQUENCE; CONFERENCE PAPER; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME ISOLATION; ENZYME STRUCTURE; GENE EXPRESSION; GENE STRUCTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROCESSING; SEQUENCE HOMOLOGY; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; ASPARTIC ENDOPEPTIDASES; ASTERACEAE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; MOLECULAR SEQUENCE DATA; PLANTS; PROTEIN PROCESSING, POST-TRANSLATIONAL; SEQUENCE HOMOLOGY, AMINO ACID;

CENTAUREA CALCITRAPA; CYNARA; CYNARA CARDUNCULUS; CYNARA CARDUNCULUS SUBSP. FLAVESCENS;

EID: 0031894720     PISSN: 00652598     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4615-5373-1_59     Document Type: Article

References (6)

1. Tang J, Wong RNS (1987) Evolution in the structure and function of aspartic proteases. J. Cell. Biochem. 33:53-63
2. Guruprasad K. Dhanaraj V, Groves M, Blundell TL. (1995) Aspartic proteinases: The structures and functions of a versatile superfamily of enzymes. Persp. Drug Dis. Des. 2: 329-341
3. Cordeiro MC, Pais MS, Brodelius PE (1997) Milk clotting enzymes from Cynara cardunculus subsp. flavescens (Cardoon).In: Biotechnology in Agriculture and Forestry (Y.P.S. Bajaj, ed.) Springer Verlag, Berlin, in press
4. Heimgartner U, Pietrzak M, Geertsen R, Brodelius P, da Silva Figueiredo AC, Pais MSS (1990) Purification and partial characterization of milk clotting proteases from flowers of Cynara cardunculus. Phytochemistry 29:1405-1410
5. Cordeiro M, Xue Z-T, Pietrzak M, Pais MS, Brodelius PE (1994) Isolation and characterization of a cDNA from flowers of Cynara cardunculus encoding cyprosin (an aspartic proteinase) and its use to study the organ-specific expression of cyprosin. Plant Molecular Biology 24:733-741
6. Ponting CP, Russell RB (1995) Swaposins: circular permutations within genes encoding saposin homologues. Trends Biol. Sci. 20: 179-180