Shotgun cloning and characterization of the thymidylate synthase- encoding gene from Mycobacterium bovis BCG

Microbiology and Immunology

Volumn 42, Issue 1, 1998, Pages 15-21

  Matsumoto, Sohkichi ^a   Yukitake, Hideharu ^a   Ohara, Naoya ^a   Dairi, Tohru ^b   Kanbara, Hiroji ^a   Yamada, Takeshi ^a  

^a NAGASAKI UNIVERSITY   (Japan)

^b TOYAMA PREFECTURAL UNIVERSITY   (Japan)

Author keywords

Gene expression; Mycobacterial enzyme; ThyA

Indexed keywords

BCG VACCINE; THYMIDYLATE SYNTHASE;

AMINO ACID SEQUENCE; ARTICLE; COMPUTER ANALYSIS; ENZYME INDUCTION; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; MOLECULAR CLONING; MYCOBACTERIUM BOVIS; NONHUMAN; OPEN READING FRAME; SEQUENCE ANALYSIS;

EID: 0031894507     PISSN: 03855600     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1348-0421.1998.tb01964.x     Document Type: Article

References (36)

1. Bashyam, M.D., Kaushal, D., Dasgupta, S.K., and Tyagi, A.K. 1996. A study of mycobacterial transcriptional apparatus: identification of novel features in promoter elements. J. Bacteriol. 178: 4847-4853.
2. Belfort, M., Maley, G., Pedersen, L.J., and Maley, F. 1983. Primary structure of the Escherichia coli thyA gene and its thymidylate synthase product. Proc. Natl. Acad. Sci. U.S.A. 80: 4914-4918.
3. Bell, P.D., Galloway, S.J., and Belfort, M. 1991. A transcription terminator in the thymidylate synthase (thyA) structural gene of Escherichia coli and construction of a viable thyA::Kmr deletion. J. Bacteriol. 173: 1193-1200.
4. Bertino, J.R. 1979. Toward improved selectivity in cancer chemotherapy: the Richard and Hinda Rosenthal Foundation Award Lecture. Cancer Res. 39: 293-304.
5. Beverley, S.M., Ellenberger, T.E., and Cordingley, J.S. 1986. Primary structure of the gene encoding the bifunctional dihydrofolate reductase-thymidylate synthase of Leishmania major. Proc. Natl. Acad. Sci. U.S.A. 83: 2584-2588.
6. Blin, P., Delolme, H.G., Heyraud, J.D., Charpak, Y., and Sentilhes, L. 1986. Evaluation of the protective effect of BCG vaccination by a case-control study in Yaounde, Cameroon. Tubercle 67: 283-288.
7. Bzik, D.J., Li, W.B., Horii, T., and Inselburg, J. 1987. Molecular cloning and sequence analysis of the Plasmodium falciparum dihydrofolate reductase-thymidylate synthase gene. Proc. Natl. Acad. Sci. U.S.A. 84: 8360-8364.
8. Chu, F.K., Maley, G.F., West, D.K., Belfort, M., and Maley, F. 1986. Characterization of the intron in the phage T4 thymidylate synthase gene and evidence for its self-excision from the primary transcript. Cell 45: 157-166.
9. Climie, S., Ruiz, P.L., Gonzalez, P.D., Prapunwattana, P., Cho, S.W., Stroud, R., and Santi, D.V. 1990. Saturation site-directed mutagenesis of thymidylate synthase. J. Biol. Chem. 265: 18776-18779.
10. Climie, S., and Santi, D.V. 1990. Chemical synthesis of the thymidylate synthase gene. Proc. Natl. Acad. Sci. U.S.A. 87: 633-637.
11. Crusberg, T.C., Leary, R., and Kisliuk, R.L. 1970. Properties of thymidylate synthetase from dichloromethotrexate-resistant Lactobacillus casei. J. Biol. Chem. 245: 5292-5296.
12. Edman, U., Edman, J.C., Lundgren, B., and Santi, D.V. 1989. Isolation and expression of the Pneumocystis carinii thymidylate synthase gene. Proc. Natl. Acad. Sci. U.S.A. 86: 6503-6507.
13. Frothingham, R., Hills, H.G., and Wilson, K.H. 1994. Extensive DNA sequence conservation throughout the Mycobacterium tuberculosis complex. J. Clin. Microbiol. 32: 1639-1643.
14. Gan, K., Sankaran, K., Williams, M.G., Aldea, M., Rudd, K.E., Kushner, S.R., and Wu, H.C. 1995. The umpA gene of Escherichia coli encodes phosphatidylglycerol:prolipoprotein diacylglyceryl transferase (lgt) and regulates thymidylate synthase levels through translational coupling. J. Bacteriol. 177: 1879-1882.
15. Grumont, R., Washtien, W.L., Caput, D., and Santi, D.V. 1986. Bifunctional thymidylate synthase-dihydrofolate reductase from Leishmania tropica: sequence homology with the corresponding monofunctional proteins. Proc. Natl. Acad. Sci. U.S.A. 83: 5387-5391.
16. Hardy, L.W., Finer, M.J., Montfort, W.R., Jones, M.O., Santi, D.V., and Stroud, R.M. 1987. Atomic structure of thymidylate synthase: target for rational drug design. Science 235: 448-455.
17. Huberts, P., and Mizrahi, V. 1995. Cloning and sequence analysis of the gene encoding the DNA polymerase I from Mycobacterium tuberculosis. Gene 164: 133-136.
18. Johnson, L.F. 1994. Posttranscriptional regulation of thymidylate synthase gene expression. J. Cell. Biochem. 54: 387-392.
19. Kunz, B.A., and Glickman, B.W. 1985. Mechanism of mutation by thymine starvation in Escherichia coli: clues from mutagenic specificity. J. Bacteriol. 162: 859-864.
20. Madhusudan, K., Ramesh, V., and Nagaraja, V. 1994. Molecular cloning of gyrA and gyrB genes of Mycobacterium tuberculosis: analysis of nucleotide sequence. Biochem. Mol. Biol. Int. 33: 651-660.
21. Maley, G.F., Bellisario, R.L., Guarino, D.U., and Maley, F. 1979. The primary structure of Lactobacillus casei thymidylate synthetase. I. The isolation of cyanogen bromide peptides 1 through 5 and the complete amino acid sequence of CNBr 1, 2, 3, and 5. J. Biol. Chem. 254: 1288-1295.
22. Matsumoto, S., Matsuo, T., Ohara, N., Hotokezaka, H., Naito, M., Minami, J., and Yamada, T. 1995. Cloning and sequencing of a unique antigen MPT70 from Mycobacterium tuberculosis H37Rv and expression in BCG using E. coli-mycobacteria shuttle vector. Scand. J. Immunol. 41: 281-287.
23. Navalgund, L.G., Rossana, C., Muench, A.J., and Johnson, L.F. 1980. Cell cycle regulation of thymidylate synthetase gene expression in cultured mouse fibroblasts. J. Biol. Chem. 255: 7386-7390.
24. Okada, N., Sasakawa, C., Tobe, T., Yamada, M., Nagai, S., Talukder, K.A., Komatsu, K., Kanegasaki, S., and Yoshikawa, M. 1991. Virulence-associated chromosomal loci of Shigella flexneri identified by random Tn5 insertion mutagenesis. Mol. Microbiol. 5: 187-195.
25. Perryman, S.M., Rossana, C., Deng, T.L., Vanin, E.F., and Johnson, L.F. 1986. Sequence of a cDNA for mouse thymidylate synthase reveals striking similarity with the prokaryotic enzyme. Mol. Biol. Evol. 3: 313-321.
26. Ponnighaus, J.M., Fine, P.E., Sterne, J.A., Wilson, R.J., Msosa, E., Gruer, P.J., Jenkins, P.A., Lucas, S.B., Liomba, N.G., and Bliss, L. 1992. Efficacy of BCG vaccine against leprosy and tuberculosis in northern Malawi. Lancet 339: 636-639.
27. Rode, W., Scanlon, K.J., Moroson, B.A., and Bertino, J.R. 1980. Regulation of thymidylate synthetase in mouse leukemia cells (L1210). J. Biol. Chem. 255: 1305-1311.
28. Ross, P., O'Gara, F., and Condon, S. 1990. Cloning and characterization of the thymidylate synthase gene from Lactococcus lactis subsp. lactis. Appl. Environ. Microbiol. 56: 2156-2163.
29. Salazar, L., Fsihi, H., de, Rossi, E., Riccardi, G., Rios, C., Cole, S.T., and Takiff, H.E. 1996. Organization of the origins of replication of the chromosomes of Mycobacterium smegmatis, Mycobacterium leprae and Mycobacterium tuberculosis and isolation of a functional origin from M. smegmatis. Mol. Microbiol. 20: 283-293.
30. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular cloning. A laboratory manual, 2nd ed, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
31. Suzuki, Y., Nagata, A., Ono, Y, and Yamada, T. 1988. Complete nucleotide sequence of the 16S rRNA gene of Mycobacterium bovis BCG. J. Bacteriol. 170: 2886-2889.
32. Takeda, Y, Ohlendorf, D.H., Anderson, W.F., and Matthews, B.W. 1983. DNA-binding proteins. Science 221: 1020-1026.
33. Takeishi, K., Kaneda, S., Ayusawa, D., Shimizu, K., Gotoh, O., and Seno, T. 1985. Nucleotide sequence of a functional cDNA for human thymidylate synthase. Nucleic Acids Res. 13: 2035-2043.
34. Tidjani, O., Amedome, A., and Ten, D.H. 1986. The protective effect of BCG vaccination of the newborn against childhood tuberculosis in an African community. Tubercle 67: 269-281.
35. Wayne, L.G. 1994. Dormancy of Mycobacterium tuberculosis and latency of disease. Eur. J. Clin. Microbiol. Infect. Dis. 13: 908-914.
36. Yang, F., Lu, G., and Rubin, H. 1996. Cloning, expression, purification and characterization of DNA topoisomerase I of Mycobacterium tuberculosis. Gene 178: 63-69.