Expression of an oncogenic mutant EGF receptor markedly increases the sensitivity of cells to an EGF-receptor-specific antibody-toxin

International Journal of Cancer

Volumn 75, Issue 6, 1998, Pages 878-884

  Schmidt, Mathias ^a   Reiser, Pia ^a   Hills, David ^b   Gullick, William J ^b   Wels, Winfried ^a  

^a TUMOR BIOLOGY CENTER   (Germany)

^b HAMMERSMITH HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR; IMMUNOTOXIN; PSEUDOMONAS EXOTOXIN; RECEPTOR ANTIBODY; TRANSFORMING GROWTH FACTOR ALPHA;

ANIMAL CELL; ARTICLE; CANCER IMMUNOTHERAPY; CELL KILLING; CELL STRAIN 3T3; CONTROLLED STUDY; DNA TRANSFECTION; DRUG CYTOTOXICITY; DRUG SENSITIVITY; DRUG TARGETING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECEPTOR BINDING;

EID: 0031893630     PISSN: 00207136     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1097-0215(19980316)75:6<878::aid-ijc10>3.0.co;2-%23     Document Type: Article

References (24)

1. EKSTRAND, A.J., LIU, L., HE, J., HAMID, M.L., LONGO, N., COLLINS, V.P. and JAMES, C.D., Altered subcellular location of an activated and tumour-associated epidermal growth factor receptor. Oncogene, 10, 1455-1460 (1995).
2. EKSTRAND, A.J., LONGO, N., HAMID, M.L., OLSON, J.J., LIU, L., COLLINS, V.P. and JAMES, C.D., Functional characterization of an EGF receptor with a truncated extracellular domain expressed in glioblastomas with EGFR gene amplification. Oncogene, 9, 2313-2320 (1994).
3. GULLICK, W.J., Prevalence of aberrant expression of the epidermal growth factor receptor in human cancers. Brit. med. Bull., 47, 87-98 (1991).
4. GULLICK, W.J., A new model for the interaction of EGF-like ligands with their receptors: the new one-two. Europ. J. Cancer, 30A, 2186 (1994).
5. GULLICK, W.J., DOWNWARD, J. and WATERFIELD, M.D., Antibodies to the autophosphorylation sites of the epidermal growth factor receptor protein-tyrosine kinase as probes of structure and function. EMBO J., 4, 2869-2877 (1985).
6. HILLS, D., ROWLINSON-BUSZA, G. and GULLICK, W.J., Specific targeting of a mutant, activated EGF receptor found in glioblastoma using a monoclonal antibody. Int. J. Cancer, 63, 537-543 (1995).
7. HUMPHREY, P.A., WONG, A.J., VOGELSTEIN, B., ZALUTSKY, M.R., FULLER, G.N., ARCHER, G.E., FRIEDMAN, H.S., KWATRA, M.M., BIGNER, S.H. and BIGNER, D.D., Anti-synthetic peptide antibody reacting at the fusion junction of deletion-mutant epidermal growth factor receptors in human glioblastoma. Proc. nat. Acad. Sci. (Wash.), 87, 4207-4211 (1990).
8. HWANG, J., FITZGERALD, D.J., ADHYA, S. and PASTAN, I., Functional domains of Pseudomonas exotoxin identified by deletion analysis of the gene expressed in E. coli. Cell, 48, 129-136 (1987).
9. KOHDA, D., ODAKA, M., LAX, I., KAWASAKI, H., SUZUKI, K., ULLRICH, A., SCHLESSINGER, J. and INAGAKI, F., A 40-kDa epidermal growth factor/transforming growth factor alpha-binding domain produced by limited proteolysis of the extracellular domain of the epidermal growth factor receptor. J. biol. Chem., 268, 1976-1981 (1993).
10. LORIMER, I.A.J., WIKSTRAND, C.J., BATRA, S.K., BIGNER, D.D. and PASTAN, I., Immunotoxins that target an oncogenic mutant epidermal growth factor receptor expressed in human tumors. Clin. Cancer Res., 1, 859-864 (1995).
11. MOSCATELLO, D.K., HOLGADO-MADRUGA, M., GODWIN, A.K., RAMIREZ, G., GUNN, G., ZOLTICK, P.W., BIEGEL, J.A., HAYES, R.L. and WONG, A.J., Frequent expression of a mutant epidermal growth factor receptor in multiple human tumors. Cancer Res., 55, 5536-5539 (1995).
12. OGATA, M., FRYLING, C.M., PASTAN, I. and FITZGERALD, D.J., Cell-mediated cleavage of Pseudomonas exotoxin between Arg279 and Gly280 generates the enzymatically active fragment which translocates to the cylosol. J. biol. Chem., 267, 25396-25401 (1992).
13. REDDY, C.C., NIYOGI, S.K., WELLS, A., WILEY, H.S. and LAUFFENBURGER, D.A., Engineering epidermal growth factor for enhanced mitogenic potency. Nature (Biotechnol.), 14, 1696-1699 (1996).
14. SCHMIDT, M., HYNES, N.E., GRONER, B. and WELS, W., A bivalent single-chain antibody-toxin specific for ErbB-2 and the EGF receptor. Int. J. Cancer, 65, 538-546 (1996).
15. SCHMIDT, M., VAKALOPOULOU, E., SCHNEIDER, D.W. and WELS, W., Construction and functional characterisation of scFv(14E1)-ETA - a novel, highly potent antibody-toxin specific for the EGF receptor. Brit. J. Cancer, 75, 1575-1584 (1997).
16. SCHMIDT, M. and WELS, W., Targeted inhibition of tumour cell growth by a bispecific single-chain toxin containing an antibody domain and TGFα. Brit. J. Cancer, 74, 853-862 (1996).
17. SUGAWA, N., EKSTRAND, A.J., JAMES, C.D. and COLLINS, V.P., Identical splicing of aberrant epidermal growth factor receptor transcripts from amplified rearranged genes in human glioblastomas. Proc. nat. Acad. Sci. (Wash.), 87, 8602-8606 (1990).
18. VIEIRA, A.V., LAMAZE, C. and SCHMID, S.L., Control of EGF receptor signaling by clathrin-mediated endocytosis. Science, 274, 2086-2089 (1996).
19. WELS, W., BEERLI, R., HELLMANN, P., SCHMIDT, M., MARTE, B.M., KORNILOVA, E.S., HEKELE, A., MENDELSOHN, J., GRONER, B. and HYNES, N.E., EGF receptor and p185erbB-2-specific single-chain antibody toxins differ in their cell-killing activity on tumor cells expressing both receptor proteins. Int. J. Cancer, 60, 137-144 (1995).
20. WELS, W., HARWERTH, I.M., MUELLER, M., GRONER, B. and HYNES, N.E., Selective inhibition of tumor cell growth by a recombinant single-chain antibody-toxin specific for the erbB-2 receptor. Cancer Res., 52, 6310-6317 (1992).
21. WIKSTRAND, C.J. and 13 OTHERS, Monoclonal antibodies against EGFRvIII are tumor specific and react with breast and lung carcinomas and malignant gliomas. Cancer Res., 55, 3140-3148 (1995).
22. WOLTJER, R.L., LUKAS, T.J. and STAROS, J.V., Direct identification of residues of the epidermal growth factor receptor in close proximity to the amino terminus of bound epidermal growth factor. Proc. nat. Acad. Sci. (Wash.), 89, 7801-7805 (1992).
23. WONG, A.J., RUPPERT, J.M., BIGNER, S.H., GRZESCHIK, C.H., HUMPHREY, P.A., BIGNER, D.S. and VOGELSTEIN, B., Structural alterations of the epidermal growth factor receptor gene in human gliomas. Proc. nat. Acad. Sci. (Wash.), 89, 2965-2969 (1992).
24. YAMAZAKI, H., OHBA, Y., TAMAOKI, N. and SHIBUYA, M., A deletion mutation within the ligand binding domain is responsible for activation of epidermal growth factor receptor gene in human brain tumors. Jpn. J. Cancer Res., 81, 773-779 (1990).