메뉴 건너뛰기
BioTechniques
Volumn 24, Issue 2, 1998, Pages 194-196
GST fusion protein expression vector for in-frame cloning and site- directed mutagenesis
Author keywords

[No Author keywords available]
Indexed keywords

HYBRID PROTEIN;
EID: 0031892389     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98242bm04     Document Type: Article
Times cited : (5)
References (9)
  • 1
    • 0023734473 scopus 로고
    • Novel non-templated nucleotide addition reactions catalysed by prokaryotic and eukaryotic DNA polymerases
    • Clark, J.M. 1988. Novel non-templated nucleotide addition reactions catalysed by prokaryotic and eukaryotic DNA polymerases. Nucleic Acids Res. 16:9677-9686.
    • (1988) Nucleic Acids Res. , vol.16 , pp. 9677-9686
    • Clark, J.M.1
  • 2
    • 0028240187 scopus 로고
    • Polishing with T4 or Pfu polymerase increases the efficiency of cloning PCR fragments
    • Costa, G.L. and M.P. Weiner. 1994. Polishing with T4 or Pfu polymerase increases the efficiency of cloning PCR fragments. Nucleic Acids Res. 22:2423.
    • (1994) Nucleic Acids Res. , vol.22 , pp. 2423
    • Costa, G.L.1    Weiner, M.P.2
  • 3
    • 0024357799 scopus 로고
    • A simple method for site-directed mutagenesis using the polymerase chain reaction
    • Hemsley, A., N. Arnheim, M.D. Toney, G. Cortopassi and D.J. Galas. 1989. A simple method for site-directed mutagenesis using the polymerase chain reaction. Nucleic Acids Res. 17:6545-6551.
    • (1989) Nucleic Acids Res. , vol.17 , pp. 6545-6551
    • Hemsley, A.1    Arnheim, N.2    Toney, M.D.3    Cortopassi, G.4    Galas, D.J.5
  • 4
    • 0030888269 scopus 로고    scopus 로고
    • The conserved residue tyrosine 34 is essential for maximal activity of iron-superoxide dismutase from Escherichia coli
    • Hunter, T., K. Ikebukuro, W.H. Bannister, J.V. Bannister and G.J. Hunter. 1997. The conserved residue tyrosine 34 is essential for maximal activity of iron-superoxide dismutase from Escherichia coli. Biochemistry 36:4925-4933.
    • (1997) Biochemistry , vol.36 , pp. 4925-4933
    • Hunter, T.1    Ikebukuro, K.2    Bannister, W.H.3    Bannister, J.V.4    Hunter, G.J.5
  • 5
    • 0026864722 scopus 로고
    • The in-vitro synthesised tomato shikimate kinase precursor is enzymatically active and is imported and processed to the mature enzyme by chloroplasts
    • Schmid, J., A. Schaller, U. Leibinger, W. Boll and N, Amrhein. 1992. The in-vitro synthesised tomato shikimate kinase precursor is enzymatically active and is imported and processed to the mature enzyme by chloroplasts. Plant J. 2:375-383.
    • (1992) Plant J. , vol.2 , pp. 375-383
    • Schmid, J.1    Schaller, A.2    Leibinger, U.3    Boll, W.4    Amrhein, N.5
  • 6
    • 0023806075 scopus 로고
    • One-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
    • Smith, D.B. and K.S. Johnson. 1988. One-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31-40.
    • (1988) Gene , vol.67 , pp. 31-40
    • Smith, D.B.1    Johnson, K.S.2
  • 7
    • 0022429789 scopus 로고
    • The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA
    • Taylor, J.W., J. Ott and F. Eckstein. 1985. The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA. Nucleic Acids Res. 13:8764-8785.
    • (1985) Nucleic Acids Res. , vol.13 , pp. 8764-8785
    • Taylor, J.W.1    Ott, J.2    Eckstein, F.3
  • 8
    • 0029926931 scopus 로고    scopus 로고
    • Protein production in three different expression vectors from a single polymerase chain reaction product
    • Winder, S.J. and J. Kendrick-Jones. 1996. Protein production in three different expression vectors from a single polymerase chain reaction product. Anal. Biochem. 236:190.
    • (1996) Anal. Biochem. , vol.236 , pp. 190
    • Winder, S.J.1    Kendrick-Jones, J.2
  • 9
    • 0020457545 scopus 로고
    • The filamentous phage (Ff) as vectors for recombinant DNA - A review
    • Zinder, N.D. and J.D. Boeke. 1982. The filamentous phage (Ff) as vectors for recombinant DNA - a review. Gene 19:1-10.
    • (1982) Gene , vol.19 , pp. 1-10
    • Zinder, N.D.1    Boeke, J.D.2

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.