Immunodetection of manganese superoxide dismutase in cultured human retroocular fibroblasts using sera directed against the thyrotropin receptor

Journal of Endocrinological Investigation

Volumn 21, Issue 1, 1998, Pages 48-55

  Burch, H B ^a   Barnes, S ^a   Nagy, E V ^a,d   Sellitti, D ^b   Burman, K D ^a,e   Bahn, R S ^c   Lahiri, S ^a  

^a WALTER REED ARMY MEDICAL CENTER   (United States)

^b UNIFORMED SERVICES UNIVERSITY OF THE HEALTH SCIENCES   (United States)

^c MAYO CLINIC   (United States)

^d UNIVERSITY OF DEBRECEN   (Hungary)

^e WASHINGTON HOSPITAL CENTER   (United States)

Author keywords

Autoimmunity; Graves' disease; Ophthalmopathy; Pathogenesis; Protein; Retroocular fibroblast; Thyroid; Thyrotropin receptor

Indexed keywords

MANGANESE SUPEROXIDE DISMUTASE; MITOCHONDRIAL ENZYME; THYROTROPIN RECEPTOR;

AMINO TERMINAL SEQUENCE; ARTICLE; AUTOIMMUNITY; CONTROLLED STUDY; FIBROBLAST CULTURE; GRAVES DISEASE; HASHIMOTO DISEASE; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; MAJOR CLINICAL STUDY; SEQUENCE HOMOLOGY;

EID: 0031891695     PISSN: 03914097     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF03347286     Document Type: Article

References (31)

1. Burch H.B., Wartofsky L. Graves' ophthalmopathy: current concepts regarding pathogenesis and management. Endocr. Rev. 14: 747, 1993.
2. Burch H.B., Bahn R.S., Gorman C., Garrity J. Ophthalmopathy. In: Braveman L.E., Utiger R.D. (Eds), The Thyroid, ed. 7. Lippincott Publishing Company, Philadelphia, 1996, p. 536.
3. Bahn R.S., Heufelder A.E. Mechanisms of disease: Pathogenesis of Graves' ophthalmopathy. N. Engl. J. Med. 329: 1468, 1993.
4. Bahn R.S., Gorman C.A., Johnson C.M., Smith T.J. Presence of antibodies in the sera of patients with Graves' disease recognizing a 23 kilodalton fibroblast protein. J. Clin. Endocrinol. Metab. 69: 622, 1989.
5. Salvi M., Miller A., Wall J.R. Human orbital tissue and thyroid membranes express a 64 kDa protein which is recognized by autoantibodies in the serum of patients with thyroid-associated ophthalmopathy FEBS 232: 135, 1988.
6. Zhang Z-G., Salvi M., Miller N., Bernard N., Arthurs B., Wall J.R. Restricted tissue reactivity of autoantibodies to a 64-kDa eye muscle membrane antigen in thyroid associated ophthalmopathy. Clin. Immunol. Immunopathol. 62: 183, 1992.
7. Burch H.B., Sellitti D., Barnes S.G., Nagy E.V., Bahn R.S., Burman K.D. TSH receptor antisera for the detection of immunoreactive protein species in retroocular fibroblasts obtained from patients with Graves' ophthalmopathy. J. Clin. Endocrinol. Metab. 78: 1384, 1994 .
8. Nagy E.V., Burch H.B., Lukes Y.G., Carr F.E, Kosugi S., Kohn L.D., Burman K.D. lmmunogenicity of a unique region of the human thyrotropin receptor. J. Endocrinol. Invest. 16: 485, 1993.
9. Ban T., Kosugi S., Kohn L.D. Specific antibody to the thyrotropin receptor identifies multiple receptor forms in membranes of cells transfected with wild-type receptor complementary deoxyribonucleic acid: characterization of their relevance to receptor synthesis, processing, structure, and function. Endocrinology 131: 815, 1992.
10. Kosugi S., Ban T., Akamizu T., Kohn L.D. Site-directed mutagenesis of a portion of the extracellular domain of the rat thyrotropin receptor important in autoimmune thyroid disease and nonhomologous with gonadotropin receptors. Relationship of functional and immunogenic domains. J. Biol. Chem. 266: 19413, 1991.
11. Endo T., Ikeda M., Ohmori M., Anzai E., Haraguchi K., Onaya T. Single subunit structure of the human thyrotropin receptor. Biochem. Biophys. Res. Commun. 187: 887, 1992.
12. Yen H.C., Oberly T.D., Vichitbandha S., Ho Y.S., St Clair D.K. The protective role of manganese superoxide dismutase against adriamycin-induced acute cardiac toxicity in transgenic mice. J. Clin. Invest. 98: 1253, 1996.
13. Oberley T.D., Coursin D.B., Cihla H.P., Oberley L.W., El-Sayyad N., Ho Y.S. Immunolocalization of manganese superoxide dismutase in normal and transgenic mice expressing the human enzyme. Histochem. J. 25: 267, 1993.
14. Fernandez J., DeMott M., Atherton D., Mische S.M. Internal protein sequence analysis: enzymatic digestion for less than 10 micrograms of protein bound to polyvinylidene difluoride or nitrocellulose membranes. Anal. Biochem. 201: 255, 1992.
15. Lane W.S., Galat A., Harding M.W., Schreiber S.L. Complete Amino acid sequence of the FK506 and rapamycin binding protein, FKBP, isolated from calf thymus. J. Prot. Chem. 10: 151, 1991.
16. Mengistu M., Lukes Y.G., Nagy E.V., Burch H.B., Carr F.E., Lahiri S., Burman K.D. TSH receptor gene expression in retroocular fibroblasts. J. Endocrinol. Invest. 17: 437, 1994.
17. Heufelder A.E., Dutton C.M., Bahn R.S. Detection of TSH receptor RNA in cultured retroocular fibroblasts from patients with Graves' ophthalmopathy and pretibial myxedema. Thyroid 3: 297, 1993.
18. Feliciello A., Porcellini A., Ciullo I., Bonavolonta G., Awedimento E.V., Fenzi G.F. Expression of thyrotropin-receptor in RNA in healthy and Graves' disease retroorbital tissues. Lancet 342: 337, 1993.
19. Paschke R., Metcalfe A., Alcade L., Vassart G., Weetman A., Ludgate M. Presence of nonfunctional thyrotropin receptor variant transcripts in retroocular and other tissues. J. Clin. Endocrinol. Metab. 79: 1234, 1994.
20. Dutton C.M., Natt N., Bahn R.S. Liquid hybridization analysis of TSH receptor mRNA in cultured fibroblasts from patients with Graves' ophthalmopathy. 69th Annual Meeting of the American Thyroid Association, San Diego, California, November, 1996, Abstract 59.
21. Endo T., Onaya T. Thyrotropin receptor is expressed in nonthyroid tissues. Nippon Rinsho 52: 969, 1994.
22. Iwase K., Nagasaki A., Kato K., Ohtani S., Tsujimura T., Inagaki A., Jimbo S., Nakai A., Masunaga R., Hamada M., Mano T., Kotake M., Miura K. Localization of Cu/Zn and Mn superoxide dismutase in various thyroid disorders. Acta Endocrinol. (Copenh.) 129: 573, 1993.
23. Pereira B., Costa Rosa L.F., Safi D.A., Bechara E.J. Control of superoxide dismutase, catalase, and glutathione peroxidase activities in rat lymphoid organs by thyroid hormones. J. Endocrinol. 140: 73, 1994.
24. 2-generating machinery. Biochemistry 30: 4880, 1991.
25. Suzuki K., Kinoshita N., Matsuda Y., Higashiyama S., Kuzuya T., Minamino T., Tada M., Taniguchi N. Elevation of immunoreactive serum Mn-superoxide dismutase in patients with myocardial infarction. Free Radic. Res. Commun. 15: 325, 1992.
26. Ohno H., Matsuura N., Ishikawa M., Sato Y., Endo Y., Taniguchi N. Serum manganese-superoxide dismutase in patients with diabetes mellitus and thyroid dysfunction as judged by an ELISA. Horm. Metab. Res. 23: 449, 1991.
27. Ritter K., Kühl R-J., Semrau F., Eiffert H., Kratzin H.D., Thomssen R. Manganese superoxide dismutase as a target of autoantibodies in acute Epstein-Barr virus infection. J. Exp. Med. 180: 1995, 1994.
28. Burch H.B., Lahiri S., Bahn R.S., Barnes S.G. Superoxide radical production stimulates retroocular fibroblast proliferation in Graves' ophthalmopathy. Exp. Eye Res. 65: 311, 1997.
29. Nagy E.V., Burch H.B., Mahoney K., Lukes Y.G., Morris J.C., Burman K.D. Graves' IgG recognizes linear epitopes in the human thyrotropin receptor. Biochem. Biophys. Res. Commun. 188: 28, 1992.
30. Vlase H., Graves P.N., Magnusson R.P., Davies T.F. Human antibodies to the thyrotropin receptor: Recognition of linear, folded, and glycosylated recombinant extracellular domain. J. Clin. Endocrinol. Metab. 80: 46, 1995.
31. Kiljanski J., Nebes V., Stachura I., Kennerdell J., Wall J.R. Should Graves' disease be considered a collagen disorder of the thyroid, skeletal muscle, and the connective tissue? Horm. Metab. Res. 27: 528, 1996.